Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   GYO_RS28160 Genome accession   NC_016047
Coordinates   1254214..1257918 (+) Length   1234 a.a.
NCBI ID   WP_014113302.1    Uniprot ID   G4NUU4
Organism   Bacillus spizizenii TU-B-10     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1249214..1262918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYO_RS28145 - 1249660..1249989 (-) 330 WP_041520913.1 four-helix bundle copper-binding protein -
  GYO_RS28150 (GYO_1365) - 1250106..1250543 (-) 438 WP_014113300.1 ferritin family protein -
  GYO_RS28155 (GYO_1366) addB 1250727..1254227 (+) 3501 WP_014113301.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  GYO_RS28160 (GYO_1367) addA 1254214..1257918 (+) 3705 WP_014113302.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  GYO_RS28165 (GYO_1368) sbcD 1257990..1259165 (+) 1176 WP_014113303.1 exonuclease subunit SbcD -
  GYO_RS28170 (GYO_1369) sbcC 1259162..1262554 (+) 3393 WP_014113304.1 exonuclease subunit SbcC -
  GYO_RS28175 (GYO_1370) hlpB 1262568..1262870 (+) 303 WP_014113305.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141355.72 Da        Isoelectric Point: 5.0729

>NTDB_id=42568 GYO_RS28160 WP_014113302.1 1254214..1257918(+) (addA) [Bacillus spizizenii TU-B-10]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLGQLDELIQHQDDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAEDETGERQPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDKTETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDQNDELYQKLETFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENDAAMHADISGHPARFAVRMIHSYD
LLDDDLEERTEEKSERLEAIRKGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTIEEAEQTVHKLYEKELLTEEQKNAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=42568 GYO_RS28160 WP_014113302.1 1254214..1257918(+) (addA) [Bacillus spizizenii TU-B-10]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTCTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAAAAATATTACTATTTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAAAAGGCG
TTTTTTGAACTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCACGTTCCCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGCGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCAAAGGAGAAGCTTCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGGGCAGCTTGATGAACT
GATTCAGCATCAGGATGATTTCACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GAGACGAGTTTGATCCTGCACTCCTTGAAGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACAAGAAGTCCTGAACAGCATTTGAAAAGCTTGGCCGACATGAAGCCTGTTATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGGATGAAACAGGTGAGCGCCAGCCGAGCGAGGCAGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAGGAAACAGGCAACCTGTTTATGGTAGGTGATGTCAAACAGTCCATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACAGGACGGAAAATTGACTTGAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATAAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGATGCAGGCGAAGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAGCTGATTTCATCACCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTCCGCTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTAAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCGCGCTTTCTGTGCTGAAGGTAATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCAATTGTCGGAGCAGATGAAAACGAGCTCTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGCGACCAGAACGACGAGCTTTACCAAAAGCTGGAAACGT
TCTACGGACATCTTCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTATGACCGTGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGGTTCGGCACAAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCTCAAACTGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATCGGACCGGCTCTTGCCAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGATGCGGCTATGCATGCTGATATATCAGGACACCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGAACGGAAGAAAAAAGCGAACGCTTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACATACCCGCATCACGAGGTCACACAAATTCGTA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCGGGCCCCCGTTAAACCTGCT
GACGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTTACACATATCCCAACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAAACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAACCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NUU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.596

100

0.966

Multiple sequence alignment