Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   G5B91_RS05225 Genome accession   NZ_CP049140
Coordinates   1085563..1087152 (-) Length   529 a.a.
NCBI ID   WP_024763096.1    Uniprot ID   -
Organism   Pseudomonas nitroreducens strain HBP1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 1080563..1092152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5B91_RS05200 (G5B91_05195) pilV 1081172..1081741 (-) 570 WP_024763091.1 type IV pilus modification protein PilV -
  G5B91_RS05205 (G5B91_05200) - 1081750..1082226 (-) 477 WP_024763092.1 GspH/FimT family pseudopilin -
  G5B91_RS05210 (G5B91_05205) - 1082378..1082890 (-) 513 WP_024763093.1 GspH/FimT family pseudopilin -
  G5B91_RS05215 (G5B91_05210) thiO 1083085..1084194 (+) 1110 WP_024763094.1 glycine oxidase ThiO -
  G5B91_RS05220 (G5B91_05215) pilR 1084210..1085547 (-) 1338 WP_024763095.1 sigma-54-dependent transcriptional regulator Regulator
  G5B91_RS05225 (G5B91_05220) pilS 1085563..1087152 (-) 1590 WP_024763096.1 sensor histidine kinase Regulator
  G5B91_RS05230 (G5B91_05225) - 1087142..1087384 (-) 243 WP_024763097.1 PP0621 family protein -
  G5B91_RS05235 (G5B91_05230) - 1087540..1088565 (-) 1026 WP_024763098.1 outer membrane protein assembly factor BamD -
  G5B91_RS05240 (G5B91_05235) rluD 1088713..1089672 (+) 960 WP_024763099.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  G5B91_RS05245 (G5B91_05240) pgeF 1089669..1090394 (+) 726 WP_024763100.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58654.91 Da        Isoelectric Point: 7.9417

>NTDB_id=424909 G5B91_RS05225 WP_024763096.1 1085563..1087152(-) (pilS) [Pseudomonas nitroreducens strain HBP1]
MVAEGISLTGQGLRVLRLYHLYRLTIGLVLVLLISSSLDDELLKLAHPELFHVGCWVYLILNILIAVLMPTPRQLLPLFI
LAALDILLLSALFYTAGGIPSGIGNLMVVAVAIANILLRGRIGLLIAAIAAINLIYLTFYLSLSQVTALNHYVQAGGLGA
LSFASALLIQALARRQQVVESIAEQRATTVANLEELNALILQRMRTGILVLGDRHRVLLANQSATTLLGGHDLPGHSLAE
YSPQLVSSLQQWQQNPALRPPSLQPAESGPTVQPSFIPLRRGNEQHILMFLEDISQIAQQAQQLKLAALGRLTAGIAHEI
RNPLGAISHAAQLLLESEELDKPDRRLTQIIQDQSRRMNLVIENVLQLSRRRQSEPQLLDLKYWLHRYAGEMREGLREGQ
QIHVETGQGTVQTRMDPHQLTQVLTNLVQNGLRYSTQKNGTGQVWLRLYRDSETDLPVLEVLDDGPGISAEQRGKLFEPF
FTTESKGTGLGLYISRELCESNQARLDYKTRDEGGSCFRITFAHPRKQS

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=424909 G5B91_RS05225 WP_024763096.1 1085563..1087152(-) (pilS) [Pseudomonas nitroreducens strain HBP1]
GTGGTCGCTGAAGGGATCTCCCTCACGGGCCAGGGCCTGCGGGTCCTGCGCCTGTACCACCTGTATCGCCTGACCATCGG
CCTGGTGCTGGTACTGCTGATCTCCAGCAGCCTGGATGACGAATTGCTCAAGCTGGCCCACCCCGAGCTTTTCCATGTCG
GCTGCTGGGTCTACCTGATCCTCAACATCCTGATCGCCGTGCTGATGCCGACACCGCGCCAGCTGCTGCCGCTGTTCATC
CTGGCAGCGCTGGACATACTCCTGCTCAGCGCGCTGTTCTATACCGCCGGCGGCATCCCCAGTGGCATCGGCAACTTGAT
GGTGGTCGCCGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGCTGATCGCCGCCATCGCCGCCATCAACC
TGATCTACCTGACCTTCTACCTGAGCCTCAGCCAGGTCACCGCCCTCAACCACTACGTGCAGGCCGGCGGCCTGGGCGCC
CTGAGCTTCGCCTCGGCCCTGCTGATCCAGGCCCTGGCTCGGCGCCAGCAGGTCGTCGAGAGCATTGCCGAGCAACGTGC
CACCACGGTCGCCAACCTCGAAGAGCTCAACGCACTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTATTGGGCGATC
GCCACCGCGTGCTGCTGGCCAACCAGAGCGCCACTACGCTGCTGGGTGGCCACGACCTGCCCGGCCACTCGCTGGCCGAG
TACAGCCCGCAGCTCGTCTCCAGCCTGCAGCAATGGCAGCAGAATCCCGCCCTGCGCCCGCCAAGCCTGCAACCTGCGGA
GTCCGGCCCGACCGTACAGCCCAGCTTCATCCCCCTGCGCCGGGGCAACGAACAGCATATCCTGATGTTCCTCGAGGACA
TCTCGCAGATCGCCCAGCAGGCCCAGCAGCTCAAGCTTGCGGCGCTTGGCCGGCTGACCGCCGGCATTGCCCATGAGATC
CGCAACCCACTGGGCGCGATCAGCCATGCGGCGCAGTTGCTGCTGGAGTCCGAAGAGCTGGACAAGCCCGACCGACGCCT
GACGCAGATCATCCAGGACCAGTCGCGGCGCATGAACCTGGTCATCGAGAACGTCCTGCAGCTGTCGCGCCGACGCCAGT
CCGAACCGCAACTGCTCGACCTGAAATACTGGCTGCACCGCTATGCCGGCGAAATGCGCGAGGGCCTGCGCGAGGGACAG
CAGATCCACGTGGAGACCGGCCAGGGCACCGTGCAGACGCGCATGGACCCGCATCAGTTGACGCAGGTACTGACCAACCT
CGTGCAGAATGGGTTACGGTACAGCACCCAGAAGAACGGCACCGGCCAGGTCTGGCTGCGCCTGTACCGCGACAGCGAGA
CCGACTTGCCCGTGCTGGAAGTGCTCGACGACGGCCCCGGCATCAGCGCCGAACAGCGGGGAAAACTGTTCGAGCCGTTC
TTCACCACCGAAAGCAAGGGCACAGGCCTTGGCCTGTACATCTCCCGCGAGCTGTGCGAAAGCAATCAGGCTCGACTGGA
CTACAAGACGCGTGACGAAGGCGGCAGTTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACAAAGCTAA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(416-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(308-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

71.321

100

0.715