Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G5S34_RS21625 Genome accession   NZ_CP049139
Coordinates   4765838..4767328 (+) Length   496 a.a.
NCBI ID   WP_102663446.1    Uniprot ID   -
Organism   Herbaspirillum frisingense strain IAC152     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4760838..4772328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S34_RS21600 (G5S34_21535) - 4762165..4763193 (+) 1029 WP_006461690.1 KpsF/GutQ family sugar-phosphate isomerase -
  G5S34_RS21605 (G5S34_21540) - 4763193..4763747 (+) 555 WP_006461689.1 KdsC family phosphatase -
  G5S34_RS21610 (G5S34_21545) lptC 4763744..4764352 (+) 609 WP_102663444.1 LPS export ABC transporter periplasmic protein LptC -
  G5S34_RS21615 (G5S34_21550) lptA 4764404..4765027 (+) 624 WP_182993022.1 lipopolysaccharide transport periplasmic protein LptA -
  G5S34_RS21620 (G5S34_21555) lptB 4765030..4765770 (+) 741 WP_006461686.1 LPS export ABC transporter ATP-binding protein -
  G5S34_RS21625 (G5S34_21560) rpoN/rpoN1 4765838..4767328 (+) 1491 WP_102663446.1 RNA polymerase factor sigma-54 Machinery gene
  G5S34_RS21630 (G5S34_21565) hpf 4767487..4767849 (+) 363 WP_006461684.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  G5S34_RS21635 (G5S34_21570) grxD 4768222..4768533 (+) 312 WP_006461683.1 Grx4 family monothiol glutaredoxin -
  G5S34_RS21640 (G5S34_21575) - 4768569..4769171 (+) 603 WP_182993023.1 UbiX family flavin prenyltransferase -
  G5S34_RS21645 (G5S34_21580) - 4769247..4769528 (+) 282 WP_182993024.1 type II toxin-antitoxin system HigB family toxin -
  G5S34_RS21650 (G5S34_21585) - 4769516..4769941 (+) 426 WP_182993025.1 helix-turn-helix domain-containing protein -
  G5S34_RS21655 (G5S34_21590) - 4770028..4770786 (-) 759 WP_006461681.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55088.57 Da        Isoelectric Point: 5.1177

>NTDB_id=424899 G5S34_RS21625 WP_102663446.1 4765838..4767328(+) (rpoN/rpoN1) [Herbaspirillum frisingense strain IAC152]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQILSDNPLLERLDDALDNSVRLLADGAVSSAPSTGVAEPAGD
SGNEAAPAATDGEASFDYQDNSPESGVSGESDWSFDDVARSGKSPDDDDARPQLEAHELTLREHLLEQIRVTARTHRDRA
LLELLTDALDESGYLEEPLEDILARLPEELGVDLEELSISLKLLQSLDPAGVGARSAGECLALQIRRLPKVPFVTRKLAL
RIVEDYLPLFAQRDFNKIKKALDCDDEDLREAQTVIRQCRPHPGAEFARDASDYVVPDVIVKQTKNGWQVMLNHEVMPKL
RVNAMYASALKQAKGEGSLSSQLQEAKWLIKNMRQRFDTILRVAQAIVERQRNFFSHGAVAMRPLVLREIADTLGLHEST
ISRVTTQKYMLTPHGMFELKYFFGSHVATETGGEASSTAIRALIKQLIGAENPQTPLSDSKIADMLAEQGMVVARRTVAK
YREVLKIPPVNLRKSL

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=424899 G5S34_RS21625 WP_102663446.1 4765838..4767328(+) (rpoN/rpoN1) [Herbaspirillum frisingense strain IAC152]
ATGAAACAGTCGCTCCAGTTACGTACCTCGCAGCACCTCGCATTGACGCCGCAACTGCAGCAGTCGATCAGGCTGCTGCA
ACTGTCGACGCTGGAACTGCACCAGGAACTGGAACAGATCCTGTCCGACAATCCCCTGCTCGAACGCCTGGACGACGCGC
TCGACAATTCGGTGCGCCTATTGGCCGATGGCGCGGTCTCTTCGGCGCCCTCCACCGGAGTGGCCGAGCCCGCTGGCGAC
AGCGGCAACGAAGCAGCTCCCGCCGCTACCGATGGCGAAGCCAGTTTCGACTACCAGGACAATAGCCCCGAGAGCGGCGT
CAGCGGCGAATCCGACTGGAGCTTCGACGACGTGGCACGCAGTGGCAAGTCGCCCGACGACGACGATGCCCGCCCGCAAC
TGGAAGCGCATGAGCTGACCCTGCGCGAACACCTGCTGGAACAGATCCGGGTCACCGCCCGCACGCACCGCGACCGTGCG
CTGCTGGAACTGCTCACCGATGCGCTGGACGAAAGTGGTTACCTGGAAGAACCGCTGGAAGACATCCTGGCGCGCCTGCC
GGAAGAGCTGGGCGTTGACCTGGAAGAACTGTCGATCTCGCTGAAGCTGCTGCAGAGCCTGGACCCGGCCGGAGTGGGTG
CGCGCAGTGCCGGTGAATGTCTGGCACTGCAGATCCGCCGCCTGCCCAAGGTGCCCTTCGTCACCCGCAAGCTGGCCTTG
CGGATCGTCGAGGATTACCTGCCGCTGTTCGCGCAACGCGACTTCAACAAGATCAAGAAGGCCCTCGATTGCGATGACGA
GGACCTGCGCGAAGCACAGACGGTGATCCGTCAGTGCCGTCCGCATCCGGGCGCCGAATTCGCGCGCGATGCTTCGGACT
ACGTGGTGCCGGACGTGATTGTCAAACAGACAAAGAACGGCTGGCAGGTCATGCTCAACCATGAGGTCATGCCCAAGCTG
CGCGTCAATGCGATGTATGCGAGTGCGCTCAAGCAGGCCAAGGGCGAAGGATCGCTGTCTTCCCAGTTACAGGAAGCCAA
GTGGCTCATCAAGAACATGCGGCAACGTTTCGATACAATTTTGCGAGTCGCGCAGGCTATTGTCGAACGTCAAAGAAACT
TCTTTTCACATGGCGCAGTCGCCATGCGGCCCCTTGTGCTGCGTGAAATTGCTGATACACTGGGTCTACACGAGAGCACA
ATCTCTCGCGTGACAACTCAAAAATACATGCTCACGCCGCACGGCATGTTCGAGTTGAAGTACTTCTTCGGTAGCCACGT
CGCGACTGAAACCGGAGGCGAAGCCTCCTCCACCGCGATACGGGCGCTGATCAAACAGTTGATAGGAGCCGAAAACCCGC
AGACCCCATTATCCGATAGCAAGATTGCGGATATGCTGGCAGAACAAGGCATGGTGGTCGCGCGACGCACAGTCGCCAAA
TACCGCGAAGTGTTGAAGATTCCGCCAGTCAATCTCCGCAAGTCCCTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-494)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.449

100

0.609