Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G5S35_RS01535 Genome accession   NZ_CP049134
Coordinates   323134..324651 (-) Length   505 a.a.
NCBI ID   WP_065062208.1    Uniprot ID   -
Organism   Paraburkholderia tropica strain IAC135     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318134..329651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S35_RS01510 (G5S35_01510) - 318860..319495 (-) 636 WP_110325383.1 LON peptidase substrate-binding domain-containing protein -
  G5S35_RS01515 (G5S35_01515) rapZ 319536..320429 (-) 894 WP_176079414.1 RNase adapter RapZ -
  G5S35_RS01520 (G5S35_01520) hprK 320639..321607 (-) 969 WP_027795920.1 HPr(Ser) kinase/phosphatase -
  G5S35_RS01525 (G5S35_01525) ptsN 321750..322205 (-) 456 WP_027815677.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G5S35_RS01530 (G5S35_01530) hpf 322603..322959 (-) 357 WP_065062206.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  G5S35_RS01535 (G5S35_01535) rpoN/rpoN1 323134..324651 (-) 1518 WP_065062208.1 RNA polymerase factor sigma-54 Machinery gene
  G5S35_RS01540 (G5S35_01540) lptB 325055..325822 (-) 768 WP_110325612.1 LPS export ABC transporter ATP-binding protein -
  G5S35_RS01545 (G5S35_01545) lptA 325846..326538 (-) 693 WP_074981486.1 lipopolysaccharide transport periplasmic protein LptA -
  G5S35_RS01550 (G5S35_01550) lptC 326570..327169 (-) 600 WP_065062214.1 LPS export ABC transporter periplasmic protein LptC -
  G5S35_RS01555 (G5S35_01555) - 327166..327732 (-) 567 WP_074981487.1 KdsC family phosphatase -
  G5S35_RS01560 (G5S35_01560) kdsD 327732..328718 (-) 987 WP_065062218.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55207.01 Da        Isoelectric Point: 4.8851

>NTDB_id=424872 G5S35_RS01535 WP_065062208.1 323134..324651(-) (rpoN/rpoN1) [Paraburkholderia tropica strain IAC135]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAISQNPLLENDDDWIASPLRVAADGSLIAQPPASSNAEPMQ
GASNGSSSSSSDSDSSDGEPRGVDEYEGLGGDANGDSSQWNLDDYGRSGSASDDDDLPPLQIHESTTTLRDHLMAQLRET
NASPRDRALITFLIESLDEDGYLSATGEEILADIPEELEVDADELNAALALLHSFDPAGVGARSASECLKLQLLRVTSSP
VRTLALEIVSNHLELLAARDFTRLRKQLKTGDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWHAELNP
DVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFVHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENTKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=424872 G5S35_RS01535 WP_065062208.1 323134..324651(-) (rpoN/rpoN1) [Paraburkholderia tropica strain IAC135]
ATGAAAGCCAGCCTCCAACTCCGCCTCTCGCAGCATCTTGCGCTGACTCCGCAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCAACGCTCGAACTGCAGCAGGAAGTCGCCACCGCGATCTCGCAGAATCCGCTGCTCGAGAACGACGACGACTGGA
TCGCGAGCCCCTTGCGCGTGGCCGCCGACGGGTCGCTCATCGCCCAGCCGCCGGCTTCTTCCAACGCGGAACCGATGCAG
GGCGCGAGCAACGGCTCGTCCTCCTCGTCGAGCGACAGCGATAGCAGCGACGGCGAACCGCGCGGCGTCGACGAATACGA
AGGACTCGGCGGCGACGCCAACGGCGACTCAAGCCAATGGAACCTCGACGACTACGGCCGCTCGGGCAGCGCCTCCGACG
ACGACGACCTGCCGCCGCTGCAAATCCACGAATCCACCACCACGCTGCGCGACCATCTGATGGCGCAGCTGCGTGAAACG
AACGCGAGCCCGCGCGACCGCGCGCTCATCACGTTCCTGATCGAATCGCTCGACGAAGACGGCTACCTGTCCGCCACGGG
CGAGGAAATTCTTGCCGATATTCCCGAAGAACTCGAAGTCGACGCCGACGAACTCAACGCGGCGCTGGCACTGCTGCACA
GCTTCGATCCCGCCGGCGTGGGCGCACGTTCCGCTTCGGAGTGTCTGAAGCTGCAATTGCTGCGCGTCACATCGTCGCCG
GTGCGCACGCTTGCACTCGAGATCGTGTCGAATCATCTGGAACTGCTGGCCGCGCGCGACTTCACGCGCTTGCGCAAGCA
ACTGAAGACCGGCGACGACGAGCTGCGCGATGCCCATGCGCTGATCCGTTCGCTAGAGCCGTTCCCGGGTGCGGCGTACG
GCAAGGCCGAAGCCGACTATGTGGTGCCGGACATCATCGTGAAGAAGGCGGGCCAGAACTGGCACGCCGAACTCAATCCG
GACGTGGTGCCGAAGCTGCGCATCAACCACCTGTACGCGAATATTCTGCGCAATAACCGCGGCGATCCGGGCAGCGGCTC
GCTGCGTCAACAGCTGCAGGAAGCGCGCTGGCTCATCAAGAATATCCAGCAGCGCTTCGAGACCATCCTGCGCGTGGCGC
AGGCGATCGTCGAACGCCAGAAGAACTTTTTCGTGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGAGGGAGATTGCT
GATACGCTGGGCCTACACGAATCAACTGTCTCGCGTGTGACAACCGGTAAATACATGCTGACTCCGTTCGGGACACTTGA
ATTTAAGTACTTCTTCGGATCGCATGTATCGACGGATACGGGGGGCGCCGCATCGTCCACCGCCATCCGCGCGCTCATCA
AGCAACTCATAGGAGCCGAGAACACCAAATCCCCTCTTTCGGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTC
GTCGTCGCGCGACGCACCGTCGCGAAATATCGCGAAGCACTGAAGATTCCGGCGGTCAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

65.613

100

0.657