Detailed information NTG0424866

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: IHN50_RS13960
• Genome accession: NZ_CP062245
• Coordinates: 2859917..2860909 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081588.1
• Uniprot ID: Q540P0
• Organism: Escherichia coli strain AML003_ev01
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2854917..2865909

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IHN50_RS13940	otnK	2855693..2856859 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
IHN50_RS13945	ygbL	2856951..2857589 (+)	639	WP_001278994.1	aldolase	-
IHN50_RS13950	ygbM	2857594..2858370 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
IHN50_RS13955	ygbN	2858459..2859823 (+)	1365	WP_000104441.1	GntP family transporter	-
IHN50_RS13960	rpoS	2859917..2860909 (-)	993	WP_000081588.1	RNA polymerase sigma factor RpoS	Regulator
IHN50_RS13965	nlpD	2860972..2862066 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
IHN50_RS13970	pcm	2862251..2862877 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
IHN50_RS13975	surE	2862871..2863632 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
IHN50_RS13980	truD	2863613..2864662 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
IHN50_RS13985	ispF	2864659..2865138 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
IHN50_RS13990	ispD	2865138..2865848 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37971.86 Da
• Isoelectric Point: 4.5886

>NTDB_id=424866 IHN50_RS13960 WP_000081588.1 2859917..2860909(-) (rpoS) [Escherichia coli strain AML003_ev01]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=424866 IHN50_RS13960 WP_000081588.1 2859917..2860909(-) (rpoS) [Escherichia coli strain AML003_ev01]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

3D structure

• AlphaFold DB: Q540P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715