Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   IHN50_RS10280 Genome accession   NZ_CP062245
Coordinates   2076236..2077663 (+) Length   475 a.a.
NCBI ID   WP_000980589.1    Uniprot ID   P04995
Organism   Escherichia coli strain AML003_ev01     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2071236..2082663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHN50_RS10240 yeeW 2071420..2071614 (+) 195 WP_000988600.1 DUF5983 family protein -
  IHN50_RS10245 - 2071660..2071740 (+) 81 Protein_2030 hypothetical protein -
  IHN50_RS10250 - 2072029..2072157 (-) 129 Protein_2031 transposase domain-containing protein -
  IHN50_RS10255 - 2072229..2072411 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  IHN50_RS10260 tmaR 2072512..2072841 (-) 330 WP_000450409.1 DUF496 family protein -
  IHN50_RS10265 yeeA 2073013..2074071 (-) 1059 WP_001200891.1 FUSC family protein -
  IHN50_RS10270 sbmC 2074269..2074742 (-) 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
  IHN50_RS10275 dacD 2074861..2076027 (-) 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  IHN50_RS10280 sbcB 2076236..2077663 (+) 1428 WP_000980589.1 exodeoxyribonuclease I Machinery gene
  IHN50_RS10285 tsuB 2077706..2077933 (-) 228 WP_000234896.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  IHN50_RS10290 tsuA 2077947..2079005 (-) 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
  IHN50_RS10295 plaP 2079184..2080542 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  IHN50_RS10300 yoeI 2080532..2080594 (-) 63 WP_010723108.1 membrane protein YoeI -
  IHN50_RS10305 yeeY 2080809..2081738 (-) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  IHN50_RS10310 yeeZ 2081784..2082608 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54500.81 Da        Isoelectric Point: 5.4700

>NTDB_id=424852 IHN50_RS10280 WP_000980589.1 2076236..2077663(+) (sbcB) [Escherichia coli strain AML003_ev01]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=424852 IHN50_RS10280 WP_000980589.1 2076236..2077663(+) (sbcB) [Escherichia coli strain AML003_ev01]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1FXX
  PDB 2QXF
  PDB 3C94
  PDB 3C95
  PDB 3HL8
  PDB 3HP9
  PDB 4HCB
  PDB 4HCC
  PDB 4JRP
  PDB 4JRQ
  PDB 4JS4
  PDB 4JS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

98.947

0.585