Detailed information NTG0424285

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: IFE20_RS14735
• Genome accession: NZ_CP062228
• Coordinates: 3028743..3029735 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain UTI89
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 3023743..3034735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IFE20_RS14715 (IFE20_14715)	ygbM	3024374..3025150 (+)	777	WP_001136908.1	HPr family phosphocarrier protein	-
IFE20_RS14720 (IFE20_14720)	ygbN	3025239..3026603 (+)	1365	WP_000104443.1	GntP family transporter	-
IFE20_RS14725 (IFE20_14725)	-	3026746..3027789 (-)	1044	WP_001161578.1	MBL fold metallo-hydrolase	-
IFE20_RS14730 (IFE20_14730)	-	3028014..3028565 (+)	552	WP_001179442.1	flavodoxin family protein	-
IFE20_RS14735 (IFE20_14735)	rpoS	3028743..3029735 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
IFE20_RS14740 (IFE20_14740)	nlpD	3029798..3030892 (-)	1095	WP_306305124.1	murein hydrolase activator NlpD	-
IFE20_RS14745 (IFE20_14745)	pcm	3031077..3031703 (-)	627	WP_000254701.1	protein-L-isoaspartate O-methyltransferase	-
IFE20_RS14750 (IFE20_14750)	surE	3031697..3032458 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
IFE20_RS14755 (IFE20_14755)	truD	3032439..3033488 (-)	1050	WP_000568928.1	tRNA pseudouridine(13) synthase TruD	-
IFE20_RS14760 (IFE20_14760)	ispF	3033485..3033964 (-)	480	WP_001219247.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
IFE20_RS14765 (IFE20_14765)	ispD	3033964..3034674 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=424285 IFE20_RS14735 WP_000081550.1 3028743..3029735(-) (rpoS) [Escherichia coli strain UTI89]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=424285 IFE20_RS14735 WP_000081550.1 3028743..3029735(-) (rpoS) [Escherichia coli strain UTI89]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715