Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   G4P54_RS13510 Genome accession   NZ_CP048852
Coordinates   2523230..2523640 (-) Length   136 a.a.
NCBI ID   WP_167872918.1    Uniprot ID   A0A6H0WMI9
Organism   Bacillus tequilensis strain EA-CB0015     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2518230..2528640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4P54_RS13475 (G4P54_13430) - 2518691..2519209 (-) 519 WP_003226126.1 YqeG family HAD IIIA-type phosphatase -
  G4P54_RS13480 (G4P54_13435) - 2519571..2519711 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  G4P54_RS13485 (G4P54_13440) - 2520061..2520792 (-) 732 WP_167872915.1 SGNH/GDSL hydrolase family protein -
  G4P54_RS13490 (G4P54_13445) - 2521050..2521268 (-) 219 Protein_2630 peptidoglycan-binding domain-containing protein -
  G4P54_RS13495 (G4P54_13450) - 2521424..2522050 (+) 627 WP_167872916.1 TVP38/TMEM64 family protein -
  G4P54_RS13500 (G4P54_13455) - 2522067..2522364 (-) 298 Protein_2632 6-phosphogluconate dehydrogenase (decarboxylating) -
  G4P54_RS13505 (G4P54_13460) - 2522476..2523198 (+) 723 WP_167872917.1 DUF308 domain-containing protein -
  G4P54_RS13510 (G4P54_13465) nucA/comI 2523230..2523640 (-) 411 WP_167872918.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  G4P54_RS13515 (G4P54_13470) - 2523835..2524185 (+) 351 Protein_2635 sigma-70 family RNA polymerase sigma factor -
  G4P54_RS13520 (G4P54_13475) - 2524325..2524795 (+) 471 WP_167872919.1 MarR family transcriptional regulator -
  G4P54_RS13525 (G4P54_13480) - 2524947..2526362 (+) 1416 WP_167872920.1 MDR family MFS transporter -
  G4P54_RS13530 (G4P54_13485) spoIVCA 2526547..2528007 (-) 1461 WP_240939786.1 site-specific DNA recombinase SpoIVCA -
  G4P54_RS21375 - 2527938..2528143 (-) 206 Protein_2639 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14919.00 Da        Isoelectric Point: 5.7354

>NTDB_id=423578 G4P54_RS13510 WP_167872918.1 2523230..2523640(-) (nucA/comI) [Bacillus tequilensis strain EA-CB0015]
MKKWLAGLFLAAAVLLCLVTPQPIKAASLYDKVLYFPLARYPETGDHIRYAIADGHSDICTIDRDGADKRREESLKGIPT
KPGFDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=423578 G4P54_RS13510 WP_167872918.1 2523230..2523640(-) (nucA/comI) [Bacillus tequilensis strain EA-CB0015]
ATGAAAAAATGGCTGGCAGGCCTGTTTCTTGCTGCGGCAGTGTTGCTTTGTTTAGTGACACCGCAGCCGATCAAAGCGGC
TTCTTTGTATGACAAAGTGCTGTATTTTCCGCTGGCTCGTTATCCGGAAACGGGAGATCATATTAGATATGCGATTGCAG
ATGGGCATTCTGATATTTGCACCATTGACAGGGATGGGGCGGACAAAAGGCGGGAGGAATCATTAAAAGGAATTCCGACG
AAGCCGGGTTTTGATCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGGGCAGGGGCTGACGTCCGATATGTGAC
GCCTTCAGACAATCGCGGGGCCGGATCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGATGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H0WMI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.87

84.559

0.515