Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   G4P54_RS05645 Genome accession   NZ_CP048852
Coordinates   1069002..1072502 (+) Length   1166 a.a.
NCBI ID   WP_167872046.1    Uniprot ID   -
Organism   Bacillus tequilensis strain EA-CB0015     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1064002..1077502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4P54_RS05625 (G4P54_05610) - 1064851..1066053 (-) 1203 WP_167872042.1 MDR family MFS transporter -
  G4P54_RS05630 (G4P54_05615) - 1066167..1067894 (+) 1728 WP_167872043.1 SgrR family transcriptional regulator -
  G4P54_RS05635 (G4P54_05620) - 1067931..1068260 (-) 330 WP_167872044.1 four-helix bundle copper-binding protein -
  G4P54_RS05640 (G4P54_05625) - 1068379..1068816 (-) 438 WP_167872045.1 ferritin family protein -
  G4P54_RS05645 (G4P54_05630) addB 1069002..1072502 (+) 3501 WP_167872046.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  G4P54_RS05650 (G4P54_05635) addA 1072489..1076193 (+) 3705 WP_167872047.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  G4P54_RS05655 (G4P54_05640) sbcD 1076266..1077441 (+) 1176 WP_167872048.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134555.39 Da        Isoelectric Point: 5.5223

>NTDB_id=423544 G4P54_RS05645 WP_167872046.1 1069002..1072502(+) (addB) [Bacillus tequilensis strain EA-CB0015]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRILQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLAGQYLHSEDYLTLLAEHIPLSEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SDEREPHELELFRMTGKTYYRLHEKAKELNLDITYKEPSGSERHTRTPELAHLEAQYETRPAIPYAEKQEAVTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKNKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFTQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVVQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSVIPPALDQVFVGNMDLSRMYDTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPHHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYEISDVWWSTYNVLMSDPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKGKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIVNAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRTLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=423544 G4P54_RS05645 WP_167872046.1 1069002..1072502(+) (addB) [Bacillus tequilensis strain EA-CB0015]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCATGGCGCATTCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACAGGTGTACAGATGCTCCTGCGAAAACTCATTGAGGAGCATAAACAGGAGTT
CAAAGTTTATCAAAAAGCGAGTGACAAAAGCGGATTCACCGCCCAGGTTGAACGGATGCTGACAGAATTTAAGCGGTATT
GTCTGGAACCGGAAGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGGGAACGTGTTTTATCTGAA
AAGCTTCATGACTTATCGATTCTGTATCAGCAGATGGAAAAAAGCCTCGCAGGGCAATATCTTCACTCAGAGGATTATTT
AACATTGCTGGCGGAGCATATTCCTCTTTCGGAGGATGTAAAGGGCGCACATATCTATGTGGACGGCTTTTATCAATTTA
CGCCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCACGCAGAGCACATCACGTTTTCGCTGACAGCGGACAAGCCG
TCTGACGAACGGGAGCCGCATGAACTGGAATTATTCAGAATGACGGGAAAAACCTATTACCGCCTGCACGAAAAGGCGAA
GGAATTAAATCTGGACATTACGTATAAAGAGCCTAGCGGGAGTGAAAGGCATACACGCACACCGGAATTGGCGCATCTAG
AGGCGCAGTATGAAACGCGTCCGGCCATCCCATATGCAGAAAAACAAGAGGCTGTTACTGTGATGCAGGCCGCAAACAGA
CGAGCAGAGCTTGAAGGCATTGCCCGGGAAATTCACGCCCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCACGCCAGCCGGAAGACTACAAAGATATGGTGAAGGAAGTGTTCGCTGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATTGAATTTATCCGCTCGAGCCTTGATGTTTTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGTTATTCCCGCTTAACGAGCCGAAGAATAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTACGCAGACCGACCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCCGCTTTTTCAGCTCCAGAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATTTAGAAGAGACGGATGTGCCGCTTAAGCTGGATCAGGAAAGACAGCGTGCTGAAGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCAGTCGTTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTAGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTGTCATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGACACCTCCTGCACTTTTGTGCTGGGGGCAAACGATGGTGTTC
TCCCGGCGCGCCCTGATGAAAACGGAGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGGTTGGGGTTGAGCTTTCC
TCAGGCGGGCGAGAGCGTCTGCTTGACGAGCATTTTCTCATCTATATGGCTTTTTCCAGTCCGTCTGACAGGCTTTACGT
CTCGTATCCGATTGCCGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTTATTAAGCGGCTGGAAGAGCTGTTTCCGC
ACCATAAGGAGCGGCTGCTGACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACTGCGAGCCAGCTTAGATTATGGACACGGGAATACGAGATCAGCGACGTTTGGTGGAGCACGTA
CAATGTGCTGATGAGCGACCCGGACAGGCTGCAATCGAAAAAACTGTTTTCAAGCCTGTTTTTCCGTAATGAAGTAAAGC
AGCTTGAACGCAGCGTGTCAAGGCAGCTTTATGGTGAACGCATTCAAGGCAGTGTGTCGAGGATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCGTCACACGGGCTGCACCTGAAGGAACGGCAATTTTTCAAGCTTGAAGCACCGGACATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGGGAGCAAAAGCTGGATTGGCGCGATTTAACGAAAG
AGCAGTGTGAACTGTTTTCCTATGATGCGGTTGAACGGCTGGCGCCGAAACTGCAAAAAGAAATTCTGCTCAGCTCAAAC
CGCCATTATTATGTGAAGGGAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATTGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTCCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAGCTCGTCGGGCGAATTGACCGCGTTGATAAAGCGGAAAGCTCAAAAGGCCTGCTTCTAAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTCTATTACGGCCTGGCGCTGCAAATGCTGACATACCTTGA
TTTATCGATTACACACTCAGCCGACTGGCTCGGAATGAGAGCGACGCCTGCGGGGGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAACCTCCCGCTCGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACGACCCTTCAAGAGGGACGGTCAAATATCGTCAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCGGACTCGGCGGCAGTCGGCGAAAAGGAATTTGAGCTGCTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCTGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCTTATAAAATGAAGAACAAGACGCCA
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGCACATTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(305-558)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.17

100

0.972