Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LSA_RS02235 Genome accession   NC_015978
Coordinates   443515..444897 (+) Length   460 a.a.
NCBI ID   WP_014081755.1    Uniprot ID   A0A5C4TJ55
Organism   Fructilactobacillus sanfranciscensis TMW 1.1304     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 438515..449897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSA_RS02210 (LSA_04440) - 438813..439484 (+) 672 WP_041817631.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
  LSA_RS02215 (LSA_04450) - 439582..440634 (+) 1053 WP_041817632.1 DUF3324 domain-containing protein -
  LSA_RS02220 (LSA_04460) - 440743..442077 (-) 1335 WP_014081752.1 C1 family peptidase -
  LSA_RS02225 (LSA_04470) rpiA 442132..442818 (-) 687 WP_014081753.1 ribose-5-phosphate isomerase RpiA -
  LSA_RS02230 (LSA_04480) - 442966..443502 (+) 537 WP_014081754.1 dUTP diphosphatase -
  LSA_RS02235 (LSA_04490) radA 443515..444897 (+) 1383 WP_014081755.1 DNA repair protein RadA Machinery gene
  LSA_RS02240 (LSA_04500) gltX 444971..446461 (+) 1491 WP_014081756.1 glutamate--tRNA ligase -
  LSA_RS02245 (LSA_04510) cysS 446568..447986 (+) 1419 WP_014081757.1 cysteine--tRNA ligase -
  LSA_RS02250 (LSA_04520) - 447986..448411 (+) 426 WP_014081758.1 Mini-ribonuclease 3 -
  LSA_RS02255 (LSA_04530) rlmB 448401..449153 (+) 753 WP_014081759.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LSA_RS02260 (LSA_04540) - 449156..449695 (+) 540 WP_041817633.1 NYN domain-containing protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 50031.36 Da        Isoelectric Point: 6.5927

>NTDB_id=42337 LSA_RS02235 WP_014081755.1 443515..444897(+) (radA) [Fructilactobacillus sanfranciscensis TMW 1.1304]
MAKASTRFVCSNCGYVSPTYLGRCPNCGEWNTFEEETVTKNDTKTANVTRVSFEGKKSRPTLIDDVDAHDAPRYKIESNE
LNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLSHYGKRVLYVTGEESADQVKMRSDRLGVKHLDNLYVFPETDMTA
IREAIANVKPDVVIIDSVQTMQEGDVASAVGSVSQVRGVTADLMNIAKTNNITIFVVGHVTKGGAIAGPKTLEHMVDTVL
YFEGDKHHAYRLLRAVKNRFGSTNELGIFEMVDTGLKEVANPSEIFLEERLHNATGSAIVVAMEGTRPILVEIQALITPS
VFGNAQRTATGVDRNRVSLIMAVLEKRAGLMLQNQDAYLKAAGGVKLVEPAIDLAMAISIASSYEDISTDPKECYIGELG
LTGEIRRVDRIEQRIREATKLGFSRVIVPKHNLDGLKMDFDIDVVGVTTIREALKVALKK

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=42337 LSA_RS02235 WP_014081755.1 443515..444897(+) (radA) [Fructilactobacillus sanfranciscensis TMW 1.1304]
ATGGCAAAGGCATCGACACGATTTGTTTGTTCTAATTGTGGTTATGTTTCACCCACTTATTTGGGACGTTGTCCTAACTG
TGGTGAGTGGAATACGTTTGAAGAAGAAACGGTTACCAAAAACGATACGAAAACAGCCAATGTAACCAGAGTTTCATTTG
AAGGGAAGAAATCAAGACCAACTTTAATTGATGATGTTGACGCTCACGATGCACCCCGTTATAAAATCGAATCGAACGAA
TTAAATCGTGTATTGGGTGGTGGAGTAGTTCCAGGGTCTTTAGTTTTAATTGGTGGTGACCCAGGAATTGGAAAGTCAAC
GTTGTTATTACAAGTTTCTGGACAATTAAGTCATTATGGTAAACGAGTTTTGTACGTTACTGGAGAAGAAAGTGCGGATC
AAGTTAAGATGCGTTCGGATCGACTTGGTGTTAAGCATCTTGATAATTTATATGTTTTCCCAGAAACTGATATGACGGCT
ATTCGGGAAGCAATTGCAAATGTCAAACCAGATGTTGTCATTATTGATTCAGTGCAAACAATGCAAGAAGGCGACGTGGC
ATCAGCAGTTGGTTCTGTTTCACAAGTCCGTGGCGTGACTGCTGATTTAATGAACATTGCGAAGACTAACAATATTACCA
TTTTTGTCGTGGGACATGTGACTAAAGGTGGAGCGATTGCTGGACCAAAGACTTTAGAGCATATGGTTGATACCGTTTTG
TATTTTGAAGGTGACAAGCATCATGCTTATCGTTTGTTGCGAGCCGTTAAAAATCGATTTGGTTCAACTAATGAATTAGG
AATTTTTGAAATGGTTGATACAGGACTAAAAGAAGTTGCTAATCCATCTGAAATCTTTTTAGAAGAACGGTTGCATAACG
CTACCGGTTCCGCGATTGTAGTTGCGATGGAAGGGACTCGTCCCATTTTAGTTGAAATCCAAGCTTTAATCACGCCCTCA
GTCTTTGGGAATGCCCAACGAACTGCGACAGGAGTTGACCGAAATCGGGTTTCTTTGATTATGGCTGTTTTGGAAAAACG
AGCAGGCTTGATGTTACAAAACCAGGATGCATATTTAAAAGCTGCTGGAGGCGTTAAGCTAGTTGAACCTGCAATTGATT
TAGCAATGGCAATCAGTATTGCTTCTAGTTATGAAGACATTAGTACTGATCCAAAAGAATGTTATATTGGTGAATTAGGT
TTGACTGGTGAAATTCGTCGGGTTGATCGAATTGAGCAACGAATTCGTGAAGCTACTAAATTAGGTTTTTCCCGTGTGAT
TGTGCCCAAACATAATTTAGATGGATTAAAAATGGATTTTGATATTGATGTGGTTGGAGTTACTACCATCCGCGAAGCTT
TGAAAGTTGCTTTAAAGAAATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-224)

Subunit ChlI of Mg-chelatase (ChlI)

(370-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C4TJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

65.132

99.13

0.646
  radA Streptococcus pneumoniae D39

65.132

99.13

0.646
  radA Streptococcus pneumoniae R6

65.132

99.13

0.646
  radA Streptococcus pneumoniae TIGR4

65.132

99.13

0.646
  radA Streptococcus mitis SK321

65.132

99.13

0.646
  radA Streptococcus mitis NCTC 12261

65.132

99.13

0.646
  radA Bacillus subtilis subsp. subtilis str. 168

61.135

99.565

0.609

Multiple sequence alignment