Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   G3T51_RS00415 Genome accession   NZ_CP048732
Coordinates   78742..80112 (-) Length   456 a.a.
NCBI ID   WP_025904845.1    Uniprot ID   A0A2T4RU06
Organism   Mammaliicoccus sciuri strain NWAF26     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 73742..85112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3T51_RS00390 cysE 74135..74776 (-) 642 WP_025904850.1 serine O-acetyltransferase -
  G3T51_RS00395 gltX 75031..76485 (-) 1455 WP_048541341.1 glutamate--tRNA ligase -
  G3T51_RS00400 ispF 76501..76980 (-) 480 WP_048541339.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  G3T51_RS00405 ispD 76973..77659 (-) 687 WP_048541338.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  G3T51_RS00410 - 77661..78719 (-) 1059 WP_048541336.1 PIN/TRAM domain-containing protein -
  G3T51_RS00415 radA 78742..80112 (-) 1371 WP_025904845.1 DNA repair protein RadA Machinery gene
  G3T51_RS00420 - 80268..82730 (-) 2463 WP_025904844.1 ATP-dependent Clp protease ATP-binding subunit -
  G3T51_RS00425 - 82747..83754 (-) 1008 WP_152291888.1 protein arginine kinase -
  G3T51_RS00430 - 83755..84291 (-) 537 WP_048541333.1 UvrB/UvrC motif-containing protein -
  G3T51_RS00435 - 84306..84767 (-) 462 WP_075577610.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50477.24 Da        Isoelectric Point: 7.7103

>NTDB_id=422556 G3T51_RS00415 WP_025904845.1 78742..80112(-) (radA) [Mammaliicoccus sciuri strain NWAF26]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEESIEHKETKGPKNTFKSDVPAQKIEKLKDVKEEDTPRIKTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNVLYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTVFHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVSGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIVIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIIPKNNIKGLEFPEGTEVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=422556 G3T51_RS00415 WP_025904845.1 78742..80112(-) (radA) [Mammaliicoccus sciuri strain NWAF26]
ATGGCCAAAAAGAAAGTAATATTTGAGTGTTTAGCCTGCGGATATCAATCTCCAAAATGGATGGGGAAATGCCCTAATTG
TGGTGCATGGAACCAAATGGAAGAATCAATAGAACATAAAGAAACTAAAGGTCCTAAAAATACATTCAAGTCAGATGTAC
CCGCACAAAAAATAGAAAAATTAAAAGACGTTAAAGAAGAAGATACACCTAGAATTAAGACTAAAATCAATGAATTTAAT
CGTGTATTAGGTGGCGGTGTTGTAGAAGGTTCGCTTGTACTTATTGGTGGTGACCCTGGTATTGGTAAATCCACATTATT
ATTACAAATTTGTGCGGCTTTATCTCAACAAAAAAATGTCTTGTATGTTTCAGGTGAAGAATCTGTAAGACAAACGAAAT
TAAGAGCTGAAAGATTAGTTGAAGATTCAGGAGAATTAAATGTTTATGCTGAAACGAATTTAGAGATTATACATAGTATG
GTTCAAAAAGAAAAACCAGATGTATTAGTTATAGATTCCATTCAAACTGTCTTCCACCCTGAAGTTACTTCTGCTCCAGG
TTCTGTTTCTCAAGTTAGAGAATGTACACAAAATTTAATGCATATCGCAAAACAAATGAACATTGCTACATTTATTGTAG
GACATGTAACGAAAGATGGACAAATTGCTGGGCCACGTTTGTTAGAACATATGGTTGATACAGTATTATATTTTGAAGGC
GATACACATCATGCATACAGAATATTAAGAGCAGTTAAAAACCGTTTTGGTTCGACAAATGAAATGGGTATTTTTGAAAT
GAAGCAAACGGGTCTTAATGAAGTTAAAAATCCGTCTGAAATGTTCTTAGAAGAAAGAACGAAACATGTATCGGGTTCGA
CTATTGTGGCAACTATGGAAGGTACGAGACCTATTTTAGTTGAAGTACAAGCTTTAGTAACACCAACGACTTTTCATAAT
CCACGAAGAATGGCAACAGGTATTGATCACAATAGATTAAGCTTATTGATGGCTGTATTAGAGAAGAAAGAGAATTTCTT
ATTACAACAACAAGACGCATATATCAAAATTGCCGGTGGTGTAAAATTAAGTGAACCCGCTGTAGATTTAAGCATTGTTA
TCGCGATAGCTTCAAGTTTCCAAGATAAACCTACTAAAGGTGATGATTGTTTCGTGGGTGAGGTTGGCTTAACAGGTGAA
GTAAGAAGGGTTGCTAGAATTGATCAACGTGTACAAGAAGCGGCTAAATTAGGATTTAAACGTATTATCATACCTAAAAA
TAATATTAAAGGTTTAGAATTCCCGGAAGGTACAGAAGTTATTGGCGTATCAACAACAAGAGAAGCATTGAAAATTGCTT
TTAATGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4RU06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.721

100

0.66
  radA Streptococcus pneumoniae Rx1

61.894

99.561

0.616
  radA Streptococcus pneumoniae D39

61.894

99.561

0.616
  radA Streptococcus pneumoniae R6

61.894

99.561

0.616
  radA Streptococcus pneumoniae TIGR4

61.894

99.561

0.616
  radA Streptococcus mitis NCTC 12261

61.894

99.561

0.616
  radA Streptococcus mitis SK321

61.894

99.561

0.616

Multiple sequence alignment