Detailed information NTG0422524

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: G3G87_RS00275
• Genome accession: NZ_CP048714
• Coordinates: 57201..58550 (-)
• Length: 449 a.a.
• NCBI ID: WP_228865417.1
• Uniprot ID: A0A4Y5MXM0
• Organism: Staphylococcus lugdunensis strain CGMH-SL36
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	39371..63721	57201..58550	within	0

Gene organization within MGE regions

Location: 39371..63721

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G3G87_RS00180	mecA	39371..41380 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
G3G87_RS00185	mecR1	41477..43234 (+)	1758	WP_000952923.1	beta-lactam sensor/signal transducer MecR1	-
G3G87_RS00190	mecI	43234..43605 (+)	372	WP_000369214.1	mecA-type methicillin resistance repressor MecI	-
G3G87_RS00195	psm-mec	43690..43758 (-)	69	WP_014532405.1	phenol-soluble modulin PSM-mec	-
G3G87_RS00200	-	44089..45261 (-)	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
G3G87_RS00205	-	45424..46554 (+)	1131	WP_010922808.1	ROK family transcriptional regulator	-
G3G87_RS00210	cstB	46668..48002 (-)	1335	Protein_39	persulfide dioxygenase-sulfurtransferase CstB	-
G3G87_RS00215	-	48034..49098 (-)	1065	WP_000438836.1	DsrE/DsrF/DrsH-like family protein	-
G3G87_RS00220	cstR	49234..49494 (+)	261	WP_000220507.1	persulfide-sensing transcriptional repressor CstR	-
G3G87_RS00225	-	49494..50132 (+)	639	Protein_42	sulfite exporter TauE/SafE family protein	-
G3G87_RS00230	-	50186..50245 (-)	60	Protein_43	DNA repair protein RadC	-
G3G87_RS00235	-	50477..51139 (-)	663	WP_001092058.1	class I SAM-dependent methyltransferase	-
G3G87_RS00240	-	51531..51590 (+)	60	WP_010959190.1	erythromycin resistance leader peptide	-
G3G87_RS00245	erm(A)	51673..52404 (+)	732	WP_001072201.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A)	-
G3G87_RS00250	-	52530..53312 (-)	783	WP_000067268.1	aminoglycoside nucleotidyltransferase ANT(9)-Ia	-
G3G87_RS00255	-	53463..53840 (-)	378	WP_000361059.1	DUF6262 family protein	-
G3G87_RS00260	-	53847..55739 (-)	1893	WP_001557544.1	site-specific integrase	-
G3G87_RS00265	-	55736..56821 (-)	1086	WP_000868132.1	tyrosine-type recombinase/integrase	-
G3G87_RS00270	-	56961..57179 (-)	219	Protein_51	recombinase family protein	-
G3G87_RS00275	ccrA	57201..58550 (-)	1350	WP_228865417.1	cassette chromosome recombinase CcrA	Machinery gene
G3G87_RS00280	cch1	58784..60577 (-)	1794	WP_228865422.1	cassette chromosome replicative helicase	-
G3G87_RS00285	ssb	60577..60873 (-)	297	WP_000273823.1	cassette chromosome ssDNA-binding protein	-
G3G87_RS00290	-	61066..62112 (-)	1047	WP_001042023.1	hypothetical protein	-
G3G87_RS00295	-	62567..63721 (+)	1155	WP_000912116.1	ATP-binding protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 52619.21 Da
• Isoelectric Point: 9.9639

>NTDB_id=422524 G3G87_RS00275 WP_228865417.1 57201..58550(-) (ccrA) [Staphylococcus lugdunensis strain CGMH-SL36]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide

• Length: 1350 bp

>NTDB_id=422524 G3G87_RS00275 WP_228865417.1 57201..58550(-) (ccrA) [Staphylococcus lugdunensis strain CGMH-SL36]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

• Recombinase (Recombinase) (169-250)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

• Resolvase, N terminal domain (Resolvase) (4-147)

3D structure

• AlphaFold DB: A0A4Y5MXM0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus N315	99.332	100	0.993
ccrA	Staphylococcus aureus COL	75.056	100	0.751