Detailed information NTG0422312

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: IEC99_RS05320
• Genome accession: NZ_CP061754
• Coordinates: 1102452..1103444 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli O18ac:H14 strain 873.10
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1097452..1108444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IEC99_RS05290 (IEC99_05290)	ispD	1097512..1098222 (+)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
IEC99_RS05295 (IEC99_05295)	ispF	1098222..1098701 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
IEC99_RS05300 (IEC99_05300)	truD	1098698..1099747 (+)	1050	WP_044863711.1	tRNA pseudouridine(13) synthase TruD	-
IEC99_RS05305 (IEC99_05305)	surE	1099728..1100489 (+)	762	WP_191028228.1	5'/3'-nucleotidase SurE	-
IEC99_RS05310 (IEC99_05310)	pcm	1100483..1101109 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
IEC99_RS05315 (IEC99_05315)	nlpD	1101249..1102389 (+)	1141	Protein_1055	murein hydrolase activator NlpD	-
IEC99_RS05320 (IEC99_05320)	rpoS	1102452..1103444 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
IEC99_RS05325 (IEC99_05325)	ygbN	1103538..1104903 (-)	1366	Protein_1057	GntP family transporter	-
IEC99_RS05330 (IEC99_05330)	ygbM	1104992..1105768 (-)	777	WP_001136918.1	HPr family phosphocarrier protein	-
IEC99_RS05335 (IEC99_05335)	ygbL	1105773..1106411 (-)	639	WP_001278994.1	aldolase	-
IEC99_RS05340 (IEC99_05340)	otnK	1106408..1107670 (-)	1263	WP_000590392.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=422312 IEC99_RS05320 WP_000081550.1 1102452..1103444(+) (rpoS) [Escherichia coli O18ac:H14 strain 873.10]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=422312 IEC99_RS05320 WP_000081550.1 1102452..1103444(+) (rpoS) [Escherichia coli O18ac:H14 strain 873.10]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715