Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G0029_RS02545 Genome accession   NZ_CP048670
Coordinates   542432..543181 (+) Length   249 a.a.
NCBI ID   WP_373691629.1    Uniprot ID   -
Organism   Acinetobacter sp. YH12138 strain YH12138_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537432..548181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0029_RS02515 (G0029_02515) - 537735..537911 (+) 177 WP_180106346.1 ATP-binding protein -
  G0029_RS02520 (G0029_02520) - 538120..539397 (+) 1278 WP_373685003.1 glycosyltransferase family 4 protein -
  G0029_RS02525 (G0029_02525) - 539589..540140 (+) 552 WP_180106348.1 phosphatase PAP2 family protein -
  G0029_RS02530 (G0029_02530) iscX 540262..540462 (+) 201 WP_078189615.1 Fe-S cluster assembly protein IscX -
  G0029_RS02535 (G0029_02535) ndk 540572..541003 (+) 432 WP_078189614.1 nucleoside-diphosphate kinase -
  G0029_RS02540 (G0029_02540) rlmN 541127..542362 (+) 1236 WP_180024403.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  G0029_RS02545 (G0029_02545) pilF 542432..543181 (+) 750 WP_373691629.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G0029_RS02550 (G0029_02550) - 543172..543966 (+) 795 WP_180106349.1 helix-turn-helix domain-containing protein -
  G0029_RS02555 (G0029_02555) ispG 543990..545111 (+) 1122 WP_078192661.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  G0029_RS02560 (G0029_02560) hisS 545115..546410 (+) 1296 WP_180005380.1 histidine--tRNA ligase -
  G0029_RS02565 (G0029_02565) - 546441..547139 (+) 699 WP_180109688.1 YfgM family protein -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28099.74 Da        Isoelectric Point: 8.6162

>NTDB_id=422188 G0029_RS02545 WP_373691629.1 542432..543181(+) (pilF) [Acinetobacter sp. YH12138 strain YH12138_T]
MALTACQTPEIGKKDPEKAVKIRTQLAVEHLKAGDLDSAKRALDQALEVDSRDSQANMMMGVLLQREGSPANLEKADSYF
KRAISADSKNSQARNNYGTYLYQVERYNDAIEQFSIAGSTLGYDQRFKALENLGRIYLKVGDVANAEKSFKQALQANRDS
YVSMLELSEIFYLNQQFPAATQMYEQFVRAVGQKNQGARALWIGIRTARANGDNLGMQVLVNQLRALFPESPEYQRYLQL
QYSTEAVWK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=422188 G0029_RS02545 WP_373691629.1 542432..543181(+) (pilF) [Acinetobacter sp. YH12138 strain YH12138_T]
TTGGCATTAACAGCATGTCAAACGCCTGAGATTGGGAAAAAAGACCCAGAGAAAGCCGTAAAAATCAGAACGCAATTGGC
TGTTGAACACCTGAAAGCAGGGGATTTGGACTCTGCTAAACGCGCATTAGATCAGGCTTTAGAGGTGGATTCTCGCGATT
CTCAGGCCAATATGATGATGGGTGTCTTACTGCAACGAGAAGGCAGTCCAGCCAACTTAGAAAAAGCAGATAGTTATTTT
AAGCGTGCAATTTCAGCAGACTCAAAAAATTCTCAAGCACGTAACAACTACGGTACGTATTTATACCAAGTTGAGCGCTA
TAATGATGCGATTGAACAGTTCAGCATCGCAGGTTCAACTTTAGGTTATGATCAACGTTTCAAAGCATTGGAAAATTTAG
GTCGAATTTACCTGAAAGTGGGTGATGTGGCGAATGCTGAAAAATCATTCAAACAAGCTTTGCAGGCTAATCGTGATTCT
TATGTTTCAATGTTAGAGTTGTCTGAAATCTTTTATTTGAATCAGCAGTTCCCTGCTGCTACACAAATGTATGAACAATT
TGTACGTGCAGTAGGACAAAAAAATCAGGGTGCGCGTGCACTCTGGATTGGCATTCGAACAGCGCGTGCCAATGGCGACA
ATTTGGGTATGCAAGTACTGGTCAACCAGTTACGTGCTTTGTTCCCAGAAAGTCCTGAATACCAACGCTATTTGCAATTA
CAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(128-159)

Tetratricopeptide repeat (TPR_16)

(25-64)

Tetratricopeptide repeat (TPR_16)

(72-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

80.488

98.795

0.795
  pilF Acinetobacter baylyi ADP1

73.279

99.197

0.727

Multiple sequence alignment