Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G0028_RS03255 Genome accession   NZ_CP048659
Coordinates   665362..666162 (+) Length   266 a.a.
NCBI ID   WP_180045225.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain YH12207_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 660362..671162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0028_RS03225 (G0028_03230) - 660698..661075 (+) 378 WP_180045222.1 hypothetical protein -
  G0028_RS03230 (G0028_03235) - 661167..662456 (+) 1290 WP_180045223.1 glycosyltransferase family 4 protein -
  G0028_RS03235 (G0028_03240) - 662486..663037 (+) 552 WP_180045224.1 phosphatase PAP2 family protein -
  G0028_RS03240 (G0028_03245) iscX 663161..663361 (+) 201 WP_130074099.1 Fe-S cluster assembly protein IscX -
  G0028_RS03245 (G0028_03250) ndk 663481..663912 (+) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  G0028_RS03250 (G0028_03255) rlmN 664082..665317 (+) 1236 WP_130074100.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  G0028_RS03255 (G0028_03260) pilF 665362..666162 (+) 801 WP_180045225.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G0028_RS03260 (G0028_03265) - 666153..666944 (+) 792 WP_180045226.1 helix-turn-helix domain-containing protein -
  G0028_RS03265 (G0028_03270) ispG 666968..668089 (+) 1122 WP_130074103.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  G0028_RS03270 (G0028_03275) hisS 668093..669388 (+) 1296 WP_130074104.1 histidine--tRNA ligase -
  G0028_RS03275 (G0028_03280) - 669417..670118 (+) 702 WP_174493321.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30242.46 Da        Isoelectric Point: 8.7419

>NTDB_id=422159 G0028_RS03255 WP_180045225.1 665362..666162(+) (pilF) [Acinetobacter piscicola strain YH12207_T]
MYKKLITVAAISCAVFLTACETTQTGSVLKKDPEKAVKVRTQLAAEYIRTHDLDAAKRSLDQALEIDSGDSTANMMMGVL
LQQEGSKVSMDKADSYFKRAISRDPDNAQARNNYGTYLYLMKRYNDAIEQLQFAGAILGYDQRYRALENLGKVYMIVGDK
ENAEKTFKQTLQVNRDSVISLIELSELLYLKQQTAQASQLYEQFVRLVGQKNQDSRALWIGIRIARANGDQMGSQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=422159 G0028_RS03255 WP_180045225.1 665362..666162(+) (pilF) [Acinetobacter piscicola strain YH12207_T]
ATGTACAAAAAATTGATCACAGTCGCTGCAATCAGTTGTGCTGTGTTCTTGACGGCTTGTGAAACGACACAAACAGGGTC
AGTTTTAAAAAAAGATCCTGAAAAAGCAGTCAAAGTACGTACTCAATTGGCTGCTGAATATATTCGAACGCATGATTTAG
ATGCCGCAAAACGTTCACTTGATCAAGCCTTAGAAATTGATTCAGGTGATTCGACTGCCAATATGATGATGGGGGTTTTA
CTGCAACAAGAAGGCAGTAAAGTCAGCATGGATAAAGCGGATAGTTATTTTAAACGTGCTATTTCCCGTGATCCAGACAA
TGCACAAGCCCGTAATAATTATGGGACTTACTTATATTTGATGAAGCGTTATAATGACGCCATTGAACAACTGCAGTTTG
CAGGTGCGATTTTAGGCTATGACCAACGCTATAGAGCTTTGGAAAATCTCGGTAAAGTCTATATGATTGTAGGCGATAAA
GAGAATGCTGAAAAAACGTTCAAGCAAACGCTACAAGTCAATCGAGATTCTGTTATATCTTTAATCGAGTTGTCAGAACT
ATTATATTTAAAACAGCAAACTGCTCAAGCCAGTCAACTGTATGAGCAGTTTGTGCGTTTAGTGGGTCAGAAAAATCAAG
ACTCAAGAGCGCTTTGGATCGGAATTCGTATCGCGAGAGCGAATGGTGATCAGATGGGGTCGCAAGTATTGGTCAATCAA
TTACGCGCACTTTTTCCTGAAAGCCAAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_17)

(97-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

71.103

98.872

0.703
  pilF Acinetobacter baylyi ADP1

65.019

98.872

0.643

Multiple sequence alignment