Detailed information    

insolico Bioinformatically predicted

Overview


Name   HI0659   Type   Machinery gene
Locus tag   GXM21_RS04665 Genome accession   NZ_CP048627
Coordinates   961122..961364 (+) Length   80 a.a.
NCBI ID   WP_008538270.1    Uniprot ID   -
Organism   Megamonas funiformis strain JCM 14723     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 958648..1011250 961122..961364 within 0


Gene organization within MGE regions


Location: 958648..1011250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GXM21_RS04650 (GXM21_04650) - 959257..960363 (-) 1107 WP_008538273.1 site-specific integrase -
  GXM21_RS04655 (GXM21_04655) - 960469..960729 (+) 261 WP_008538272.1 DUF4160 domain-containing protein -
  GXM21_RS04660 (GXM21_04660) - 960764..961117 (+) 354 WP_008538271.1 DUF2442 domain-containing protein -
  GXM21_RS04665 (GXM21_04665) HI0659 961122..961364 (+) 243 WP_008538270.1 helix-turn-helix transcriptional regulator Machinery gene
  GXM21_RS04670 (GXM21_04670) - 961409..962506 (-) 1098 WP_008538269.1 SAP domain-containing protein -
  GXM21_RS04675 (GXM21_04675) - 962561..963010 (-) 450 WP_163604675.1 helix-turn-helix domain-containing protein -
  GXM21_RS04680 (GXM21_04680) - 963136..963333 (+) 198 WP_008538267.1 helix-turn-helix domain-containing protein -
  GXM21_RS04685 (GXM21_04685) - 963423..963575 (+) 153 WP_008538266.1 hypothetical protein -
  GXM21_RS04690 (GXM21_04690) - 963596..964624 (+) 1029 WP_008538265.1 hypothetical protein -
  GXM21_RS04695 (GXM21_04695) - 964627..964923 (+) 297 WP_008538264.1 hypothetical protein -
  GXM21_RS04700 (GXM21_04700) - 964962..965453 (+) 492 WP_008538263.1 hypothetical protein -
  GXM21_RS04705 (GXM21_04705) - 965458..965874 (+) 417 WP_154645558.1 hypothetical protein -
  GXM21_RS04710 (GXM21_04710) - 966012..967985 (+) 1974 WP_008538260.1 ATP-binding protein -
  GXM21_RS04715 (GXM21_04715) - 968001..968804 (+) 804 WP_008538259.1 hypothetical protein -
  GXM21_RS04720 (GXM21_04720) - 968824..969633 (+) 810 WP_008538258.1 MBL fold metallo-hydrolase -
  GXM21_RS04725 (GXM21_04725) - 969638..970099 (+) 462 WP_008538257.1 hypothetical protein -
  GXM21_RS04730 (GXM21_04730) - 970101..970709 (+) 609 WP_008538256.1 hypothetical protein -
  GXM21_RS04735 (GXM21_04735) - 970725..971567 (+) 843 WP_163604676.1 DnaD domain protein -
  GXM21_RS04740 (GXM21_04740) - 971554..972159 (+) 606 WP_008538254.1 ATP-binding protein -
  GXM21_RS04745 (GXM21_04745) - 972164..972472 (+) 309 WP_008538253.1 hypothetical protein -
  GXM21_RS04750 (GXM21_04750) - 972474..972758 (+) 285 WP_008538252.1 hypothetical protein -
  GXM21_RS04755 (GXM21_04755) - 972803..973180 (+) 378 WP_008538251.1 hypothetical protein -
  GXM21_RS04760 (GXM21_04760) - 973190..973648 (+) 459 WP_008538250.1 DUF3850 domain-containing protein -
  GXM21_RS04765 (GXM21_04765) - 973626..974129 (+) 504 WP_008538249.1 hypothetical protein -
  GXM21_RS04770 (GXM21_04770) - 974122..974619 (+) 498 WP_008538247.1 hypothetical protein -
  GXM21_RS04775 (GXM21_04775) - 974626..974808 (+) 183 WP_008538245.1 hypothetical protein -
  GXM21_RS04780 (GXM21_04780) - 974798..975226 (+) 429 WP_008538244.1 hypothetical protein -
  GXM21_RS04785 (GXM21_04785) - 975244..975717 (+) 474 WP_008538243.1 YopX family protein -
  GXM21_RS04790 (GXM21_04790) - 975970..976173 (+) 204 WP_008538241.1 hypothetical protein -
  GXM21_RS04795 (GXM21_04795) - 976385..976879 (+) 495 WP_008538239.1 sigma-70 family RNA polymerase sigma factor -
  GXM21_RS04800 (GXM21_04800) - 977139..977894 (+) 756 WP_163604677.1 ParB N-terminal domain-containing protein -
  GXM21_RS04805 (GXM21_04805) - 977887..978753 (+) 867 WP_008538237.1 hypothetical protein -
  GXM21_RS04810 (GXM21_04810) - 978837..979778 (+) 942 WP_008538236.1 radical SAM protein -
  GXM21_RS04815 (GXM21_04815) - 979824..980255 (+) 432 WP_008538234.1 hypothetical protein -
  GXM21_RS04820 (GXM21_04820) - 980242..981654 (+) 1413 WP_008538233.1 hypothetical protein -
  GXM21_RS04825 (GXM21_04825) - 981728..983143 (+) 1416 WP_211373393.1 phage portal protein -
  GXM21_RS04830 (GXM21_04830) - 983140..984108 (+) 969 WP_008538231.1 hypothetical protein -
  GXM21_RS04835 (GXM21_04835) - 984126..985421 (+) 1296 WP_050900407.1 hypothetical protein -
  GXM21_RS04840 (GXM21_04840) - 985440..986138 (+) 699 WP_008538229.1 DUF2190 family protein -
  GXM21_RS04845 (GXM21_04845) - 986152..987186 (+) 1035 WP_008538228.1 hypothetical protein -
  GXM21_RS04850 (GXM21_04850) - 987199..987561 (+) 363 WP_008538227.1 hypothetical protein -
  GXM21_RS04855 (GXM21_04855) - 987558..987947 (+) 390 WP_008538226.1 hypothetical protein -
  GXM21_RS04860 (GXM21_04860) - 987958..988371 (+) 414 WP_008538225.1 hypothetical protein -
  GXM21_RS04865 (GXM21_04865) - 988375..989241 (+) 867 WP_008538223.1 DUF3168 domain-containing protein -
  GXM21_RS04870 (GXM21_04870) - 989241..989465 (+) 225 WP_008538222.1 hypothetical protein -
  GXM21_RS04875 (GXM21_04875) - 989482..990948 (+) 1467 WP_008538220.1 phage tail sheath subtilisin-like domain-containing protein -
  GXM21_RS04880 (GXM21_04880) - 990963..991397 (+) 435 WP_008538219.1 phage tail tube protein -
  GXM21_RS04885 (GXM21_04885) - 991421..991915 (+) 495 WP_008538218.1 hypothetical protein -
  GXM21_RS04890 (GXM21_04890) - 992087..995092 (+) 3006 WP_008538216.1 phage tail tape measure protein -
  GXM21_RS04895 (GXM21_04895) - 995097..995873 (+) 777 WP_008538215.1 LysM peptidoglycan-binding domain-containing protein -
  GXM21_RS04900 (GXM21_04900) - 995870..996895 (+) 1026 WP_008538214.1 hypothetical protein -
  GXM21_RS04905 (GXM21_04905) - 996879..997262 (+) 384 WP_008538213.1 hypothetical protein -
  GXM21_RS04910 (GXM21_04910) - 997265..997717 (+) 453 WP_008538211.1 DUF2634 domain-containing protein -
  GXM21_RS13005 - 997879..998841 (+) 963 Protein_942 baseplate J/gp47 family protein -
  GXM21_RS04925 (GXM21_04925) - 998831..999844 (+) 1014 WP_163604678.1 putative phage tail protein -
  GXM21_RS04930 (GXM21_04930) - 999841..1000314 (+) 474 WP_008538206.1 hypothetical protein -
  GXM21_RS12900 - 1000324..1001976 (+) 1653 WP_008538205.1 pyocin knob domain-containing protein -
  GXM21_RS04940 (GXM21_04940) - 1002373..1002600 (+) 228 WP_039881330.1 hypothetical protein -
  GXM21_RS04945 (GXM21_04945) - 1002602..1002739 (-) 138 WP_154645555.1 hypothetical protein -
  GXM21_RS04950 (GXM21_04950) - 1004404..1004679 (+) 276 WP_008538203.1 hypothetical protein -
  GXM21_RS04955 (GXM21_04955) - 1004684..1005094 (+) 411 WP_008538202.1 hypothetical protein -
  GXM21_RS12835 - 1005087..1005251 (+) 165 WP_008538201.1 hypothetical protein -
  GXM21_RS04960 (GXM21_04960) - 1005371..1006420 (-) 1050 WP_163604679.1 tyrosine-type recombinase/integrase -
  GXM21_RS04965 (GXM21_04965) - 1006465..1006803 (+) 339 WP_008538197.1 hypothetical protein -
  GXM21_RS04970 (GXM21_04970) - 1006805..1006987 (+) 183 WP_008538196.1 hypothetical protein -
  GXM21_RS04975 (GXM21_04975) - 1007285..1007833 (+) 549 WP_008538195.1 N-acetylmuramoyl-L-alanine amidase -
  GXM21_RS04980 (GXM21_04980) - 1007844..1008050 (+) 207 WP_008538194.1 hypothetical protein -
  GXM21_RS04990 (GXM21_04990) - 1008580..1009845 (+) 1266 WP_008538193.1 SLC13 family permease -
  GXM21_RS04995 (GXM21_04995) - 1009910..1011250 (+) 1341 WP_008538192.1 serine hydroxymethyltransferase -

Sequence


Protein


Download         Length: 80 a.a.        Molecular weight: 8954.69 Da        Isoelectric Point: 10.7106

>NTDB_id=421946 GXM21_RS04665 WP_008538270.1 961122..961364(+) (HI0659) [Megamonas funiformis strain JCM 14723]
MNDIKINNLRIAIINELIRAREEQGISQKKLEELSGVKQPVIARIEKGKSIPNTDTLVKLLTPLGKKLVIVPLETVKNTK

Nucleotide


Download         Length: 243 bp        

>NTDB_id=421946 GXM21_RS04665 WP_008538270.1 961122..961364(+) (HI0659) [Megamonas funiformis strain JCM 14723]
ATGAATGATATTAAAATAAATAATTTACGTATAGCAATAATTAATGAATTAATAAGAGCTAGAGAGGAACAAGGAATAAG
TCAAAAAAAGCTGGAAGAGCTTAGCGGAGTAAAACAGCCTGTTATTGCTCGTATTGAAAAAGGTAAATCTATTCCTAATA
CAGATACACTCGTAAAATTACTTACACCATTAGGTAAAAAACTGGTTATTGTTCCGCTAGAAACAGTAAAAAATACTAAA
TAA

Domains


Predicted by InterproScan.

(19-68)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  HI0659 Haemophilus influenzae Rd KW20

63.636

96.25

0.612


Multiple sequence alignment