Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   G3M69_RS00540 Genome accession   NZ_CP048599
Coordinates   115983..118346 (-) Length   787 a.a.
NCBI ID   WP_163562721.1    Uniprot ID   -
Organism   Helicobacter pylori strain GCT 97     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 110983..123346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M69_RS00515 (G3M69_00515) lpxE 111515..112048 (+) 534 WP_240329120.1 lipid A 1-phosphatase LpxE -
  G3M69_RS00520 (G3M69_00520) nspC 112070..113287 (-) 1218 WP_163562719.1 carboxynorspermidine decarboxylase -
  G3M69_RS00525 (G3M69_00525) cheV1 113284..114249 (-) 966 WP_000785489.1 chemotaxis protein CheV1 -
  G3M69_RS00530 (G3M69_00530) - 114349..115689 (-) 1341 WP_163562720.1 COG3014 family protein -
  G3M69_RS00540 (G3M69_00540) comB4 115983..118346 (-) 2364 WP_163562721.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  G3M69_RS00545 (G3M69_00545) comB3 118348..118611 (-) 264 WP_078216260.1 competence protein ComB Machinery gene
  G3M69_RS00550 (G3M69_00550) comB2 118611..118892 (-) 282 WP_001272686.1 TrbC/VirB2 family protein Machinery gene
  G3M69_RS00555 (G3M69_00555) - 119039..119866 (-) 828 WP_163562722.1 DUF5718 family protein -
  G3M69_RS00560 (G3M69_00560) - 119960..121012 (-) 1053 WP_163562723.1 MnmA/TRMU family protein -
  G3M69_RS00565 (G3M69_00565) dnaG 121009..122688 (-) 1680 WP_163562724.1 DNA primase -
  G3M69_RS00570 (G3M69_00570) groES 122977..123333 (+) 357 WP_000671934.1 co-chaperone GroES -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90246.73 Da        Isoelectric Point: 9.0023

>NTDB_id=421660 G3M69_RS00540 WP_163562721.1 115983..118346(-) (comB4) [Helicobacter pylori strain GCT 97]
MLEKLLNAVKQKVSNYFLGVLPKSYSMSEENNILGLYDEHFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVARLVVKRRKIDYRQSIQSDSKHLQAILNQFENKEVYENRYFLVLESTHSLHGVLEHKKKSFMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLNSKEVLNFYAEYINGFELPLKPLVGGYLSDSYIASSITFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAVGALLYQETPLDIIFSIEPMSTHKTLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNILIKAPSLEDLDAQTSLILGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLVIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGQSFSINPFCLEPNLQNLEFLQSFFLSMFDLAPSRDKEALEDMNAVSGAIKSLYETLYPKAFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDAFNSNAFLNVINLDAITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNTKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHNFNVPLSETEIS
FLENTSLYARQVLVKNLGNGSSNMIDVSLEGLGRYLKIFNSDSSHVNKVKALQKDYPTEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=421660 G3M69_RS00540 WP_163562721.1 115983..118346(-) (comB4) [Helicobacter pylori strain GCT 97]
ATGTTAGAAAAGCTTTTGAACGCTGTCAAACAAAAAGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACATTTTAGGCTTGTATGATGAGCATTTTTTACTCACTAAAAACGAAAACTTAGTGGGCATCCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACAGAGCAATTGCAAGATCTTTTCACAGAGCGCCAAATGGCGTTGGAT
TCTTTAGAAAAAGTCGTGGCGCGCCTGGTGGTTAAAAGGCGTAAAATTGATTATAGACAAAGCATTCAATCTGATTCTAA
ACACTTGCAAGCGATCTTGAACCAGTTTGAAAACAAAGAAGTGTATGAAAATCGGTATTTTTTAGTTTTAGAAAGCACTC
ACTCTTTGCATGGGGTTTTAGAGCATAAGAAAAAATCTTTCATGCACGCTAATAGAGAAAATTTTAAGGATATTCTCTCT
TATAAAGCGCATTTTTTGCAAGAAACTTTAAAAAGCCTAGAAATCCAGCTCAAAAACTATGCCCCCAAACTCTTAAACTC
TAAAGAGGTTTTGAATTTTTATGCAGAATACATTAATGGGTTTGAACTCCCTTTAAAGCCTCTAGTAGGAGGGTATTTGA
GCGATAGCTATATCGCTAGTTCTATCACTTTTGAAAAAGATTACTTCATTCAAGAAAGTTTTAATCAAAAAACCTACAAC
CGCTTGATTGGTATTAAAGCTTATGAGAGCGAACGTATCACTTCTATAGCGGTGGGAGCGCTTTTATACCAAGAGACGCC
TTTGGATATTATCTTTTCTATAGAGCCTATGAGCACGCATAAAACGCTGAGTTTTTTAAAGGAGAGAGCCAAGTTTAGCA
TGTCTAATCTTGTTAAAAACGAGCTGCTAGAATACCAAGAATTAGTCAAAACCAAACGCTTATCCATGCAAAAATTCGCC
TTAAACATTCTTATCAAAGCCCCCAGTTTAGAGGATTTAGACGCTCAAACGAGCTTAATTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAAGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCACTTAAACC
ACCGCTTGCGTTTTTTAACCTCTAAAGCCCTAGCGTGTTTGATGGTGTTTGAAAGGCAAAATTTAGGCTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAATAACGCTAAAGAAATCGCCAGAGTGAATGGGCATACTTTAGTTATAGGGGCAACCGGAAGCGGTAAAAGCA
CGCTGATTAGCTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCCTTTGACAGGATGCAAGGGCTG
TATTCTTTCACCAAATTTTTTAAAGGGCATTACCATGACGGCCAATCTTTTAGCATCAACCCCTTTTGTTTAGAGCCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTTTTTTTGAGCATGTTTGATCTCGCCCCTTCAAGGGATAAAGAAGCCTTAG
AAGACATGAATGCGGTTTCTGGTGCGATTAAGAGCCTTTATGAGACCTTATACCCTAAAGCCTTTAGTTTGCTAGACTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAACTGGGCTTGAGTTTAGAGCCGTATTTGAATAACCCCCTTTTTAACGC
TTTGAATGACGCGTTCAACTCCAACGCTTTTTTAAACGTGATAAACCTAGATGCGATCACCCAAAACCCTAAAGATTTAG
GGCTTTTAGCCTATTATTTGTTTTATAAGATCTTAGAAGAGTCCAGGAAAAACGACAGCGGCTTTTTGGTTTTTTTAGAC
GAGTTTAAATCCTATGTGGAAAACGATTTATTGAACACTAAAATCAACGCTTTAATCACGCAAGCCAGGAAAGCTAATGG
CGTGGTGGTGTTGGCCTTGCAAGATATTTACCAACTCAGCGGGGTTAAAAACGCCCATAGTTTTTTAAGCAACATGGGGA
CTCTCATTTTGTATCCGCAAAAAAACGCTAGGGAGTTGAAACACAACTTCAATGTGCCTTTGAGCGAAACTGAAATTTCA
TTTTTAGAAAACACCTCTCTGTATGCCAGGCAGGTTTTAGTCAAAAATCTGGGTAACGGGAGCTCTAACATGATTGATGT
GAGTTTGGAGGGCTTGGGGCGTTATTTGAAGATCTTTAATTCAGATTCTAGCCATGTCAATAAAGTGAAAGCGTTACAAA
AAGACTACCCTACAGAGTGGCGTGAGAAACTTTTGAAGAGCTAG

Domains

Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

96.95

100

0.97

Multiple sequence alignment