Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G0D89_RS13485 Genome accession   NZ_CP048451
Coordinates   2880458..2881963 (+) Length   501 a.a.
NCBI ID   WP_006398255.1    Uniprot ID   -
Organism   Burkholderia multivorans strain P1Bm2012b     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2875458..2886963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G0D89_RS13460 (G0D89_13500) kdsD 2876709..2877692 (+) 984 WP_006406620.1 arabinose 5-phosphate isomerase KdsD -
  G0D89_RS13465 (G0D89_13505) - 2877692..2878228 (+) 537 WP_006398259.1 KdsC family phosphatase -
  G0D89_RS13470 (G0D89_13510) lptC 2878232..2878846 (+) 615 WP_006398258.1 LPS export ABC transporter periplasmic protein LptC -
  G0D89_RS13475 (G0D89_13515) lptA 2878867..2879532 (+) 666 WP_006398257.1 lipopolysaccharide transport periplasmic protein LptA -
  G0D89_RS13480 (G0D89_13520) lptB 2879529..2880305 (+) 777 WP_006398256.1 LPS export ABC transporter ATP-binding protein -
  G0D89_RS13485 (G0D89_13525) rpoN/rpoN1 2880458..2881963 (+) 1506 WP_006398255.1 RNA polymerase factor sigma-54 Machinery gene
  G0D89_RS13490 (G0D89_13530) hpf 2882122..2882481 (+) 360 WP_006398254.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  G0D89_RS13495 (G0D89_13535) - 2882677..2883188 (+) 512 Protein_2642 PTS sugar transporter subunit IIA -
  G0D89_RS13500 (G0D89_13540) hprK 2883317..2884285 (+) 969 WP_006398252.1 HPr(Ser) kinase/phosphatase -
  G0D89_RS13505 (G0D89_13545) rapZ 2884364..2885272 (+) 909 WP_006409809.1 RNase adapter RapZ -
  G0D89_RS13510 (G0D89_13550) - 2885300..2885935 (+) 636 WP_006415469.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54746.94 Da        Isoelectric Point: 4.8740

>NTDB_id=421438 G0D89_RS13485 WP_006398255.1 2880458..2881963(+) (rpoN/rpoN1) [Burkholderia multivorans strain P1Bm2012b]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPVSNGEPAI
ANGNGQSSDRAERDEPAGADEYDGYAAGDASDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=421438 G0D89_RS13485 WP_006398255.1 2880458..2881963(+) (rpoN/rpoN1) [Burkholderia multivorans strain P1Bm2012b]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCGGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGTGTCGAACGGCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGAGCGATCGTGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTATGCGGC
CGGCGACGCGAGCGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCCTCCGATGACGACGATCTGC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGATCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCGAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAGGTGCT
CGCCGATCTGCCGCCCGAACTCGAAGTCGACTGCGATGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGTTCGGCATCGGAGTGTCTGAAGCTGCAGTTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAACATCTGGAACTGCTCGCCGCGCGCGATTTCACGCGGTTGCGCAAGCATCTGAAGGCCAC
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCAGCCTACGGCAAGGCCGAAG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAAGTCGGCCAGGGCTGGCAGGCCGAGCTCAATCCCGAGGTCGTGCCG
AAGCTGCGCATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGCTCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTCATCAAGAACATCCAGCAGCGGTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACGGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCCAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695

Multiple sequence alignment