Detailed information NTG0421409

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: G0D91_RS13610
• Genome accession: NZ_CP048445
• Coordinates: 2899489..2900631 (+)
• Length: 380 a.a.
• NCBI ID: WP_006416170.1
• Uniprot ID: A0A0H3KRG3
• Organism: Burkholderia multivorans strain P1Bm2015
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2894489..2905631

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G0D91_RS13580 (G0D91_13620)	-	2894884..2895489 (+)	606	WP_012212698.1	50S ribosomal protein L25/general stress protein Ctc	-
G0D91_RS13585 (G0D91_13625)	pth	2895598..2896197 (+)	600	WP_006398241.1	aminoacyl-tRNA hydrolase	-
G0D91_RS13590 (G0D91_13630)	hisC	2896333..2897400 (+)	1068	WP_206380727.1	histidinol-phosphate transaminase	-
G0D91_RS13595 (G0D91_13635)	-	2897467..2897730 (-)	264	WP_006406632.1	YfhL family 4Fe-4S dicluster ferredoxin	-
G0D91_RS13600 (G0D91_13640)	coaD	2898052..2898549 (-)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
G0D91_RS13605 (G0D91_13645)	rsmD	2898661..2899275 (-)	615	WP_039220118.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
G0D91_RS13610 (G0D91_13650)	pilA	2899489..2900631 (+)	1143	WP_006416170.1	signal recognition particle-docking protein FtsY	Machinery gene
G0D91_RS13615 (G0D91_13655)	maiA	2900702..2901346 (-)	645	WP_039220123.1	maleylacetoacetate isomerase	-
G0D91_RS13620 (G0D91_13660)	ybiB	2901520..2902491 (+)	972	WP_206380728.1	DNA-binding protein YbiB	-
G0D91_RS13625 (G0D91_13665)	leuA	2902884..2904530 (+)	1647	WP_206380729.1	2-isopropylmalate synthase	-
G0D91_RS13630 (G0D91_13670)	-	2904666..2905049 (-)	384	WP_006398232.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 380 a.a.
• Molecular weight: 40336.19 Da
• Isoelectric Point: 4.9462

>NTDB_id=421409 G0D91_RS13610 WP_006416170.1 2899489..2900631(+) (pilA) [Burkholderia multivorans strain P1Bm2015]
MFSFFKRFKKTQEPGSAESQPADAPQPDAPSDAHDADVPPAPAPAEPAAPAVVMTVTPSDNGRDEVVETVEIVPPPVQDA
SAKKSWLARLKTGLAKTGSSITGVFVNTKIDDALYEELETALLMSDAGVDATEYLLGALREKVRVGRLTDPQQVKSALRD
LLVELLTPLEKSLVLGRAQPLVMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLTVWGERNNVTVVQ
QESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVIAKAHDGAPHEVLLVIDANTGQNALTQVKAF
DDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAEEFADALLG

Nucleotide

• Length: 1143 bp

>NTDB_id=421409 G0D91_RS13610 WP_006416170.1 2899489..2900631(+) (pilA) [Burkholderia multivorans strain P1Bm2015]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGGCTCCGCGGAATCGCAGCCGGCCGACGCGCCGCAACC
CGACGCCCCGTCCGACGCGCACGACGCCGACGTTCCGCCTGCACCCGCGCCTGCCGAACCGGCCGCGCCCGCCGTCGTGA
TGACGGTCACGCCGAGCGACAACGGCCGCGACGAAGTCGTCGAGACCGTCGAAATCGTGCCGCCGCCCGTGCAGGATGCA
TCGGCGAAGAAATCGTGGCTCGCGCGCCTGAAGACGGGCCTCGCGAAAACCGGCTCGAGCATCACCGGCGTATTCGTGAA
CACGAAGATCGACGACGCGCTGTACGAGGAGCTCGAAACCGCGCTGCTGATGTCCGATGCCGGCGTCGACGCGACGGAGT
ACCTGCTCGGCGCGCTGCGCGAAAAGGTGCGGGTGGGCCGCCTGACCGACCCGCAGCAGGTGAAGAGCGCGCTGCGCGAC
CTGCTCGTCGAGCTGCTGACGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGTGCGCAGCCGCTCGTGATGATGATCGCGGG
CGTCAACGGCGCCGGCAAGACGACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGACCAGTCGGTGCTGCTCG
CCGCGGGCGATACGTTCCGCGCCGCGGCGCGCGAACAGCTGACGGTCTGGGGCGAACGCAACAACGTGACCGTCGTGCAG
CAGGAAAGCGGCGACCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAAGATCGACGTGATGATGGC
CGACACGGCCGGCCGCCTGCCGACGCAGCTTCATCTGATGGAAGAGCTGAAGAAGGTGAAGCGCGTGATCGCGAAGGCAC
ACGACGGCGCGCCGCACGAAGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCACGCAGGTGAAGGCATTC
GACGATGCGCTCGGCCTGACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGGATTCTCGCCGCGATCGC
GCGGCAGCGGCCGGTGCCCGTGTACTTCATCGGCGTCGGCGAAAAGGTCGAGGATCTGCAGCCGTTCAGCGCCGAAGAAT
TCGCGGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (98-160)

• SRP54-type protein, GTPase domain (SRP54) (181-380)

3D structure

• AlphaFold DB: A0A0H3KRG3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.846	100	0.589