Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GXM20_RS11210 Genome accession   NZ_CP048436
Coordinates   2294292..2295665 (-) Length   457 a.a.
NCBI ID   WP_009256858.1    Uniprot ID   A0A174AVX6
Organism   Flavonifractor plautii strain JCM 32125     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2285061..2299107 2294292..2295665 within 0


Gene organization within MGE regions


Location: 2285061..2299107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GXM20_RS11165 (GXM20_11165) ligA 2285061..2287061 (+) 2001 WP_007489512.1 NAD-dependent DNA ligase LigA -
  GXM20_RS11170 (GXM20_11170) - 2287142..2287675 (+) 534 WP_007489511.1 amidase domain-containing protein -
  GXM20_RS11175 (GXM20_11175) - 2287803..2289326 (+) 1524 WP_007489510.1 hypothetical protein -
  GXM20_RS11180 (GXM20_11180) - 2289366..2290394 (-) 1029 WP_024724088.1 tyrosine recombinase XerC -
  GXM20_RS11185 (GXM20_11185) - 2290494..2291018 (-) 525 WP_007489507.1 tyrosine-type recombinase/integrase -
  GXM20_RS11190 (GXM20_11190) - 2291195..2291506 (-) 312 WP_007489506.1 hypothetical protein -
  GXM20_RS11195 (GXM20_11195) - 2291599..2292582 (-) 984 WP_007489505.1 hypothetical protein -
  GXM20_RS11200 (GXM20_11200) - 2293329..2293553 (-) 225 WP_007488969.1 hypothetical protein -
  GXM20_RS11205 (GXM20_11205) - 2293714..2293959 (+) 246 WP_007488970.1 helix-turn-helix transcriptional regulator -
  GXM20_RS11210 (GXM20_11210) radA 2294292..2295665 (-) 1374 WP_009256858.1 DNA repair protein RadA Machinery gene
  GXM20_RS11215 (GXM20_11215) - 2295662..2296414 (-) 753 WP_007488973.1 N-acetylmuramoyl-L-alanine amidase -
  GXM20_RS11220 (GXM20_11220) - 2296542..2297000 (+) 459 WP_024724093.1 YhcH/YjgK/YiaL family protein -
  GXM20_RS11225 (GXM20_11225) - 2297187..2297645 (+) 459 WP_024724094.1 spore coat protein CotJB -
  GXM20_RS11230 (GXM20_11230) - 2297646..2298242 (+) 597 WP_007488978.1 manganese catalase family protein -
  GXM20_RS11235 (GXM20_11235) - 2298406..2299107 (+) 702 WP_035300162.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49061.50 Da        Isoelectric Point: 5.8607

>NTDB_id=421331 GXM20_RS11210 WP_009256858.1 2294292..2295665(-) (radA) [Flavonifractor plautii strain JCM 32125]
MKAKTLFYCTECGNETPKWSGQCPACRAWNTLVERPAEPKKRSAAAASVAGGGLRGTGNRPRPMREVETTHELRFETGMN
ELDRVLGGGAVKGSLVLVGGAPGIGKSTLMLQICDNLCRFASVLYVSGEESERQIKLRAERLHVRGEGMYLLAETNLEDV
LESVNELKPDVLIVDSIQTLYNGDLTTAPGSVGQVKDCTMALMQLAKGQGITVFVIGHVNKEGSIAGPKVLEHMVDCVLY
FEGEQQNSYRILRAAKNRFGATNEIGVFEMEDSGLSEVPNPSEMLLSGRPQDTPGTCVTCVMEGVRPVLAEVQALLAPTS
FNVPRRTANGFDFNRANLLLAVLEKRGGLMVSSCDAYINVIGGLFLDEPAADLAMIVALASSFRDKPVSGDLAAIGEVGL
TGELRSVNALGQRLSEVRRLGFTKCLIPARTGGKLDTPDGLQLIPVRNIREALAALL

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=421331 GXM20_RS11210 WP_009256858.1 2294292..2295665(-) (radA) [Flavonifractor plautii strain JCM 32125]
ATGAAAGCAAAAACACTGTTTTATTGTACCGAGTGCGGAAATGAAACCCCAAAGTGGTCCGGCCAATGTCCCGCCTGCCG
GGCATGGAATACCCTTGTGGAGCGGCCGGCAGAGCCGAAAAAAAGGAGTGCGGCCGCCGCCTCTGTCGCTGGGGGCGGCC
TGCGGGGAACCGGCAACCGGCCCAGGCCCATGCGCGAGGTGGAAACCACCCACGAGCTCCGCTTTGAAACCGGGATGAAT
GAGCTGGACCGGGTGCTGGGCGGGGGAGCGGTGAAGGGCTCTCTGGTGCTGGTGGGGGGTGCCCCCGGCATCGGCAAGTC
CACCCTGATGCTCCAGATCTGCGACAACCTGTGCCGTTTTGCCTCTGTGCTCTATGTCTCCGGGGAGGAGTCGGAGCGGC
AGATCAAGCTGCGGGCGGAGCGGCTCCATGTCCGTGGAGAGGGAATGTACCTTCTGGCTGAGACCAACCTGGAGGATGTG
CTGGAGTCGGTCAACGAGCTGAAGCCAGACGTGCTAATTGTGGACTCCATCCAGACCCTCTACAACGGGGATCTGACCAC
CGCTCCCGGCAGCGTGGGACAGGTAAAGGACTGTACTATGGCGCTGATGCAGTTGGCGAAGGGGCAGGGGATCACCGTCT
TTGTCATCGGACACGTCAATAAGGAGGGGTCTATCGCAGGCCCCAAGGTGCTGGAGCACATGGTGGACTGCGTGCTCTAT
TTCGAAGGTGAGCAGCAGAACTCCTACCGCATTCTGCGCGCGGCAAAGAACCGTTTCGGCGCCACCAATGAGATCGGCGT
CTTTGAGATGGAGGACTCCGGCCTGAGCGAAGTGCCCAATCCATCAGAGATGCTGCTCTCGGGACGGCCTCAGGACACCC
CTGGCACCTGCGTCACCTGCGTCATGGAGGGAGTGCGGCCTGTTTTGGCCGAGGTACAGGCCCTGCTGGCGCCCACCAGC
TTCAATGTACCGCGCAGGACCGCCAACGGCTTCGACTTCAACCGGGCGAATCTGTTGCTGGCTGTTCTGGAAAAGCGGGG
TGGACTGATGGTCAGTTCCTGTGACGCCTATATTAACGTCATCGGCGGCTTGTTTTTGGACGAGCCCGCCGCCGACCTAG
CCATGATCGTCGCACTGGCTTCCAGCTTCCGAGACAAGCCCGTGTCTGGAGATCTGGCCGCCATCGGCGAGGTTGGCCTG
ACCGGAGAGCTGCGCTCAGTCAATGCGTTGGGCCAGCGGCTGAGCGAGGTGCGCCGTCTGGGGTTCACAAAGTGCCTGAT
CCCAGCCCGTACCGGCGGAAAGCTGGACACCCCGGATGGCCTGCAGCTCATTCCGGTTCGCAACATCCGCGAGGCGCTGG
CCGCGCTTTTGTAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(366-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)

AAA domain (AAA_25)

(72-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A174AVX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.851

99.781

0.527
  radA Streptococcus pneumoniae Rx1

50

99.781

0.499
  radA Streptococcus pneumoniae D39

50

99.781

0.499
  radA Streptococcus pneumoniae R6

50

99.781

0.499
  radA Streptococcus pneumoniae TIGR4

50

99.781

0.499
  radA Streptococcus mitis NCTC 12261

50

99.781

0.499
  radA Streptococcus mitis SK321

50

99.781

0.499

Multiple sequence alignment