Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   D8K17_RS03120 Genome accession   NZ_CP061322
Coordinates   573993..574634 (-) Length   213 a.a.
NCBI ID   WP_003131746.1    Uniprot ID   Q9CI22
Organism   Lactococcus lactis subsp. lactis bv. diacetylactis strain S50     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 568993..579634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8K17_RS03100 (D8K17_003110) cysK 569024..569956 (-) 933 WP_010905432.1 cysteine synthase A -
  D8K17_RS13870 - 570174..570317 (+) 144 WP_223203842.1 hypothetical protein -
  D8K17_RS03105 (D8K17_003115) - 570321..571259 (+) 939 WP_223203843.1 LysM peptidoglycan-binding domain-containing protein -
  D8K17_RS03110 (D8K17_003120) - 571298..571930 (-) 633 WP_003131748.1 MBL fold metallo-hydrolase -
  D8K17_RS03115 (D8K17_003125) - 571931..573925 (-) 1995 WP_003131747.1 transglycosylase domain-containing protein -
  D8K17_RS03120 (D8K17_003130) recU 573993..574634 (-) 642 WP_003131746.1 Holliday junction resolvase RecU Machinery gene
  D8K17_RS03125 (D8K17_003135) - 574715..575239 (+) 525 WP_010905434.1 DUF1273 domain-containing protein -
  D8K17_RS03130 (D8K17_003140) spx 575399..575785 (+) 387 WP_010905435.1 transcriptional regulator Spx -
  D8K17_RS03135 (D8K17_003145) - 575877..576158 (+) 282 WP_003131743.1 UPF0223 family protein -
  D8K17_RS03140 (D8K17_003150) - 576124..576903 (+) 780 WP_017864167.1 inositol monophosphatase family protein -
  D8K17_RS03145 (D8K17_003155) murA 576997..578277 (+) 1281 WP_031296728.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  D8K17_RS03150 (D8K17_003160) - 578277..578471 (+) 195 WP_010905437.1 DNA-directed RNA polymerase subunit beta -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 24369.13 Da        Isoelectric Point: 10.1344

>NTDB_id=421040 D8K17_RS03120 WP_003131746.1 573993..574634(-) (recU) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
MVNYPNGRSRAYSTVPKKKKSLTELSVTKKSTSKTKGMVAFGKRGMNFEAEINATNEYYLSRGLAVIHKKPTPIQIVKVD
YPQRSRAKITEAYFRQASTTDYSGVYKGHYIDFEAKETQQKTVFPLKNFHEHQIVHMSNVLAQGGIAFVLLHFARLNETY
LLPSSCLITFYYEKGGLKSIPLSHIRENGYKIETNHIPRIPYLEIVNKLCEVQ

Nucleotide


Download         Length: 642 bp        

>NTDB_id=421040 D8K17_RS03120 WP_003131746.1 573993..574634(-) (recU) [Lactococcus lactis subsp. lactis bv. diacetylactis strain S50]
ATGGTTAATTATCCTAATGGAAGGTCGCGAGCTTACAGCACTGTTCCTAAGAAAAAAAAATCACTGACAGAGCTGTCAGT
AACTAAAAAAAGCACCTCAAAAACCAAAGGGATGGTTGCCTTCGGGAAGCGGGGAATGAATTTTGAAGCTGAAATTAATG
CAACAAATGAGTATTATCTTTCTCGTGGACTTGCAGTTATTCACAAAAAGCCAACACCAATTCAAATCGTTAAAGTAGAT
TATCCACAAAGGAGTCGTGCAAAAATTACAGAAGCTTACTTCAGGCAAGCATCTACTACTGACTACTCTGGAGTCTATAA
AGGTCACTACATCGACTTTGAAGCAAAGGAAACACAGCAAAAAACTGTTTTCCCTCTAAAAAATTTCCATGAGCATCAAA
TTGTTCACATGAGTAATGTTCTCGCTCAAGGGGGAATTGCTTTTGTCCTCTTGCACTTTGCAAGGTTAAATGAAACATAT
CTTCTTCCATCTTCGTGTTTGATTACTTTTTATTACGAAAAAGGCGGTTTAAAGTCAATTCCGCTTTCTCATATTCGCGA
AAATGGCTATAAAATTGAAACGAACCACATTCCTAGAATTCCTTATCTAGAAATTGTGAATAAACTTTGTGAGGTACAGT
AA

Domains

Predicted by InterProScan.

Recombination protein U (RecU)

(44-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CI22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

47.393

99.061

0.469