Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   I14_RS21370 Genome accession   NC_017652
Coordinates   4332203..4334032 (+) Length   609 a.a.
NCBI ID   WP_000035581.1    Uniprot ID   Q3YVE4
Organism   Escherichia coli str. 'clone D i14'     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4327203..4339032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I14_RS21345 (i14_4332) - 4327983..4328480 (-) 498 WP_000823790.1 DUF943 family protein -
  I14_RS21350 (i14_4333) - 4328477..4329325 (-) 849 WP_001059676.1 YPO3983 family protein -
  I14_RS21355 (i14_4334) rarD 4329636..4330526 (-) 891 WP_000339101.1 EamA family transporter RarD -
  I14_RS21360 (i14_4335) yigI 4330578..4331045 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  I14_RS21365 (i14_4336) pldA 4331209..4332078 (+) 870 WP_001259692.1 phospholipase A -
  I14_RS21370 (i14_4338) recQ 4332203..4334032 (+) 1830 WP_000035581.1 ATP-dependent DNA helicase RecQ Machinery gene
  I14_RS21375 (i14_4339) rhtC 4334096..4334716 (+) 621 WP_000928839.1 threonine export protein RhtC -
  I14_RS21380 (i14_4340) rhtB 4334777..4335397 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  I14_RS21385 (i14_4341) pldB 4335508..4336530 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  I14_RS21390 (i14_4342) yigL 4336538..4337338 (+) 801 WP_000285366.1 sugar/pyridoxal phosphate phosphatase YigL -
  I14_RS21395 (i14_4343) bioP 4337414..4338313 (+) 900 WP_001196237.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68363.51 Da        Isoelectric Point: 7.2544

>NTDB_id=42089 I14_RS21370 WP_000035581.1 4332203..4334032(+) (recQ) [Escherichia coli str. 'clone D i14']
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=42089 I14_RS21370 WP_000035581.1 4332203..4334032(+) (recQ) [Escherichia coli str. 'clone D i14']
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACCGTACTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTCTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTAGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAGCTTGAAGT
AATGACAGGCTGCCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGTTTTCCGACGCTCCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGCCAGGATATTGTGCGCCTGCTGGGGCTGAACGATCCGCTAATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTACAGGAACAACGCGGTAAGTCGGGAATT
ATCTACTGCAACAGCCGCGCAAAAGTAGAAGACACCGCTGCACGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAAAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAACAAGCCTAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACTGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGTCGTCGTTTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGCGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCGGAGCAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGTATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGTGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391

Multiple sequence alignment