Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GZH82_RS12655 Genome accession   NZ_CP048279
Coordinates   2658547..2659917 (-) Length   456 a.a.
NCBI ID   WP_162682786.1    Uniprot ID   A0A6P1X3B1
Organism   Staphylococcus sp. MI 10-1553     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2653547..2664917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH82_RS12630 (GZH82_12630) cysE 2653712..2654377 (-) 666 WP_155262047.1 serine O-acetyltransferase -
  GZH82_RS12635 (GZH82_12635) gltX 2654765..2656219 (-) 1455 WP_037543943.1 glutamate--tRNA ligase -
  GZH82_RS12640 (GZH82_12640) ispF 2656296..2656775 (-) 480 WP_162682783.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GZH82_RS12645 (GZH82_12645) ispD 2656768..2657466 (-) 699 WP_162682784.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GZH82_RS12650 (GZH82_12650) - 2657467..2658528 (-) 1062 WP_162682785.1 PIN/TRAM domain-containing protein -
  GZH82_RS12655 (GZH82_12655) radA 2658547..2659917 (-) 1371 WP_162682786.1 DNA repair protein RadA Machinery gene
  GZH82_RS12660 (GZH82_12660) - 2660467..2662926 (-) 2460 WP_162682787.1 ATP-dependent Clp protease ATP-binding subunit -
  GZH82_RS12665 (GZH82_12665) - 2662942..2663946 (-) 1005 WP_162682788.1 protein arginine kinase -
  GZH82_RS12670 (GZH82_12670) - 2663936..2664466 (-) 531 WP_014614778.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50044.56 Da        Isoelectric Point: 7.1750

>NTDB_id=420565 GZH82_RS12655 WP_162682786.1 2658547..2659917(-) (radA) [Staphylococcus sp. MI 10-1553]
MAKTKTIFECTACGYQSPKWMGKCPNCGAWNSMEESFEQKTASPKHGVRAQKTSSAKIQKLNEIKQELAPRIQTNSGELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSKEKRVLYITGEESLNQTKIRADRLDEDASQLNVFAETDLMVIHEA
VKKVEPDLIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNVATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVLNPSEMFLEERTTNVAGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVRLSEPAVDLGIVIATASSFKDQAVDGLDCYIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKNNMGGWQFPEGIEVIGVTSVNEALKYALKN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=420565 GZH82_RS12655 WP_162682786.1 2658547..2659917(-) (radA) [Staphylococcus sp. MI 10-1553]
GTGGCAAAAACGAAAACAATTTTTGAATGTACGGCCTGTGGTTACCAATCACCTAAATGGATGGGAAAATGTCCGAATTG
CGGTGCTTGGAATTCAATGGAGGAATCTTTTGAACAAAAAACTGCGAGTCCTAAACACGGTGTAAGGGCGCAAAAGACAA
GTTCGGCCAAAATACAAAAGCTCAATGAAATTAAACAAGAACTTGCGCCTCGTATCCAAACGAATAGTGGTGAATTAAAC
CGTGTGCTCGGTGGCGGTATTGTAGAAGGGTCACTCGTGCTGATTGGTGGCGATCCAGGAATAGGGAAGTCTACACTGTT
ACTTCAAATGTGTGCGGCATTATCTAAAGAAAAAAGAGTGCTCTATATCACTGGAGAAGAATCACTTAATCAAACTAAAA
TTAGAGCGGATCGTTTAGATGAAGATGCGAGTCAATTAAACGTGTTTGCGGAAACGGACTTAATGGTTATTCATGAAGCG
GTGAAAAAAGTCGAGCCTGATTTGATTGTAGTGGACTCTATTCAAACGATTTACCATCCCGAAATTAGTTCGGCGCCTGG
CTCTGTGTCTCAAGTTCGTGAAAGTACGCAAAGTTTGATGCATATCGCAAAACAAATGAATGTTGCAACGTTTATTGTAG
GCCATGTGACGAAAGAAGGCCAAATTGCGGGGCCACGTTTGCTTGAACATATGGTAGATACGGTGCTCTATTTTGAAGGC
GATGAACATCATGCGTATCGTATTTTAAGAGCGGTGAAAAACCGTTTTGGCTCTACGAATGAAATGGGCATTTTTGAAAT
GAAGCAGTCCGGTTTAAAAAGTGTGCTCAATCCGTCAGAAATGTTTTTAGAAGAACGGACGACCAATGTTGCGGGTTCGA
CGATTGTCGCAACGATGGAAGGGACACGACCATTGTTAATTGAAGTGCAAGCGCTTGTCACACCGACGACGTTTAATAAT
CCGAGACGTATGGCGACAGGTATTGACCATAATCGCCTCAGTTTGTTGATGGCGGTGTTAGAGAAAAAAGAAGGTTACTT
ATTACAGCAACAAGACGCTTACATTAAGGTTGCAGGGGGTGTACGACTTTCTGAGCCCGCTGTAGACTTAGGTATCGTCA
TTGCGACAGCTTCTAGTTTTAAAGATCAAGCAGTAGACGGCCTCGATTGTTATATTGGCGAGGTTGGATTGACTGGTGAG
GTACGTCGTGTTTCGCGTATTGAGCAACGTGTGCAAGAAGCAGCAAAACTGGGCTTTAAACGCGTGATTATCCCTAAAAA
CAATATGGGTGGATGGCAGTTTCCAGAAGGTATTGAAGTGATTGGCGTTACTTCTGTAAATGAAGCATTGAAATACGCAT
TAAAAAATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

AAA domain (AAA_25)

(69-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1X3B1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.57

100

0.656
  radA Streptococcus pneumoniae Rx1

58.407

99.123

0.579
  radA Streptococcus pneumoniae D39

58.407

99.123

0.579
  radA Streptococcus pneumoniae R6

58.407

99.123

0.579
  radA Streptococcus pneumoniae TIGR4

58.407

99.123

0.579
  radA Streptococcus mitis NCTC 12261

58.407

99.123

0.579
  radA Streptococcus mitis SK321

58.407

99.123

0.579

Multiple sequence alignment