Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M655_RS27405 Genome accession   NZ_CP048274
Coordinates   5553913..5555280 (-) Length   455 a.a.
NCBI ID   WP_005828909.1    Uniprot ID   A0A9X7TE40
Organism   Brevibacillus sp. NSP2.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5548913..5560280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M655_RS27375 ispF 5548952..5549425 (-) 474 WP_005828904.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  M655_RS27380 ispD 5549429..5550115 (-) 687 WP_005828905.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M655_RS27385 (M655_027010) - 5550150..5551238 (-) 1089 WP_005828906.1 PIN/TRAM domain-containing protein -
  M655_RS27390 (M655_027015) - 5551527..5551922 (+) 396 WP_005828907.1 hypothetical protein -
  M655_RS27395 (M655_027020) pssA 5552016..5552744 (-) 729 WP_005828908.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  M655_RS27400 (M655_027025) disA 5552862..5553903 (-) 1042 Protein_5375 DNA integrity scanning diadenylate cyclase DisA -
  M655_RS27405 (M655_027030) radA 5553913..5555280 (-) 1368 WP_005828909.1 DNA repair protein RadA Machinery gene
  M655_RS27410 (M655_027035) - 5555391..5557841 (-) 2451 WP_005828910.1 ATP-dependent Clp protease ATP-binding subunit -
  M655_RS27415 (M655_027040) - 5557912..5558982 (-) 1071 WP_005828911.1 protein arginine kinase -
  M655_RS27420 (M655_027045) - 5559003..5559521 (-) 519 WP_005828912.1 UvrB/UvrC motif-containing protein -
  M655_RS27425 (M655_027050) - 5559551..5560015 (-) 465 WP_025845270.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49484.97 Da        Isoelectric Point: 6.8639

>NTDB_id=420518 M655_RS27405 WP_005828909.1 5553913..5555280(-) (radA) [Brevibacillus sp. NSP2.1]
MAKYKTKYACQECGYESPKWMGKCPGCNSWNTLVEEVTVKAAHRHEGLSGGTRQSAIPLTQVASEEEPRMDTTIGELNRV
LGGGLVPGSLILVGGDPGIGKSTLLLQTSFALAHQGAKVLYVSGEESAKQIKLRADRLHMQTPPMFVLAENDLDLIEQHI
NQVDPHVLIIDSIQTVFHPAVQSAAGSVAQVREATAQLMRIAKGKGICTFIVGHVTKEGSIAGPRMLEHMVDAVLYFEGE
RHNTFRILRAVKNRFGSTNEIGIFEMKDRGLEEVANPSEIFLAERPFGVAGSTVVASMEGTRPVLVELQALVAPTSFVTP
RRMATGVDHQRVAMIMAVLEKRMGMMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEIGLTGEV
RGVSRIEQRVREAHKLGFKRVIIPEKNIRGLDAPDDIQVIGVSNIADALNEVIRR

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=420518 M655_RS27405 WP_005828909.1 5553913..5555280(-) (radA) [Brevibacillus sp. NSP2.1]
ATGGCAAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCAAAATGGATGGGAAAATGCCCGGGCTG
CAATAGCTGGAATACATTGGTGGAAGAAGTGACAGTCAAGGCGGCGCACCGCCACGAAGGGCTGAGCGGCGGCACGCGCC
AGTCGGCTATCCCTTTGACGCAGGTAGCCAGCGAAGAAGAGCCGCGCATGGATACGACCATTGGCGAGTTGAACCGCGTG
CTCGGGGGAGGGCTCGTCCCAGGCTCGCTCATCCTGGTCGGCGGCGATCCGGGTATCGGCAAGTCCACACTGCTGTTGCA
GACCTCTTTTGCTCTGGCTCATCAGGGGGCCAAAGTGCTGTACGTATCCGGGGAGGAGTCCGCGAAGCAGATCAAGCTGC
GCGCAGACCGGCTGCATATGCAGACGCCCCCGATGTTTGTATTGGCAGAAAATGATTTGGATTTGATCGAACAGCATATT
AATCAAGTAGACCCTCATGTGTTGATTATCGACTCGATCCAGACCGTGTTCCATCCCGCTGTGCAGTCTGCGGCCGGAAG
CGTCGCGCAGGTGCGCGAGGCGACGGCCCAGTTGATGCGGATTGCCAAGGGCAAAGGAATCTGTACTTTTATTGTCGGCC
ACGTGACCAAAGAAGGCTCGATCGCAGGTCCTCGTATGCTTGAACATATGGTGGACGCAGTTCTCTATTTTGAAGGCGAG
CGCCACAATACGTTCCGGATTTTGCGCGCTGTCAAAAACCGTTTTGGTTCGACAAACGAGATCGGAATTTTCGAGATGAA
GGATCGCGGTCTGGAAGAGGTCGCAAACCCTTCCGAGATTTTTCTGGCTGAGCGTCCCTTCGGCGTGGCTGGCTCCACCG
TCGTAGCCAGCATGGAAGGCACCCGTCCAGTCCTGGTGGAGCTTCAGGCACTCGTGGCACCGACCAGCTTCGTTACGCCC
CGGAGAATGGCTACAGGCGTCGATCACCAGCGTGTGGCGATGATTATGGCTGTTTTGGAAAAGCGCATGGGCATGATGCT
GCAAAATCAGGACGCGTATGTCAACGTGGCCGGGGGCGTCCGCCTGGATGAGCCTGCTGTCGATTTGGCGATCGCCGTCA
GCATCGCCAGCAGCTTTCGCGACCATGCCACCAATCCGCACGACGTCGTGATCGGGGAGATTGGCCTGACAGGAGAGGTG
CGGGGCGTATCGCGCATTGAACAGCGCGTGCGGGAAGCGCACAAGCTCGGCTTCAAACGCGTGATTATACCGGAGAAAAA
CATTCGCGGCCTGGATGCGCCAGACGACATTCAGGTGATCGGCGTCAGCAATATTGCAGATGCTTTGAACGAGGTGATAA
GGAGGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(337-427)

AAA domain (AAA_25)

(71-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.814

99.341

0.664
  radA Streptococcus mitis NCTC 12261

57.837

99.56

0.576
  radA Streptococcus pneumoniae Rx1

57.837

99.56

0.576
  radA Streptococcus pneumoniae D39

57.837

99.56

0.576
  radA Streptococcus pneumoniae R6

57.837

99.56

0.576
  radA Streptococcus pneumoniae TIGR4

57.837

99.56

0.576
  radA Streptococcus mitis SK321

57.837

99.56

0.576

Multiple sequence alignment