Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   M654_RS00520 Genome accession   NZ_CP048273
Coordinates   89768..90880 (-) Length   370 a.a.
NCBI ID   WP_026589494.1    Uniprot ID   A0A6I7F816
Organism   Bacillus sp. NSP9.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 84768..95880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M654_RS00505 (M654_000505) gyrA 84855..87332 (-) 2478 WP_026589497.1 DNA gyrase subunit A -
  M654_RS00510 (M654_000510) gyrB 87535..89448 (-) 1914 WP_035428614.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  M654_RS00515 (M654_000515) - 89507..89749 (-) 243 WP_026589495.1 extracellular matrix/biofilm biosynthesis regulator RemA family protein -
  M654_RS00520 (M654_000520) recF 89768..90880 (-) 1113 WP_026589494.1 DNA replication/repair protein RecF Machinery gene
  M654_RS00525 (M654_000525) yaaA 90896..91111 (-) 216 WP_026589493.1 S4 domain-containing protein YaaA -
  M654_RS00530 (M654_000530) dnaN 91280..92416 (-) 1137 WP_026589492.1 DNA polymerase III subunit beta -
  M654_RS00535 (M654_000535) dnaA 92591..93931 (-) 1341 WP_026589491.1 chromosomal replication initiator protein DnaA -
  M654_RS00540 (M654_000540) rpmH 94570..94704 (+) 135 WP_003178075.1 50S ribosomal protein L34 -
  M654_RS00545 (M654_000545) rnpA 94790..95149 (+) 360 WP_026589490.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42390.32 Da        Isoelectric Point: 6.8971

>NTDB_id=420414 M654_RS00520 WP_026589494.1 89768..90880(-) (recF) [Bacillus sp. NSP9.1]
MYIQNLTLSSYRNYERLELQFENKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDKELIRWDEDYAKIEGRVMKKNGS
VPIQLVISKKGKKGKVNHIEQQKLSQYVGAVNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDQTMLDVLTEQLAEFAAKVVIKRLQFVDQLEKWAQPIHSGISRGLEELTLKYHTSLHVSDSPDLSKM
INSYQEAFSKLRDKEIDRGVSLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIQEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHKTLNEAAIFRVENGTLLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=420414 M654_RS00520 WP_026589494.1 89768..90880(-) (recF) [Bacillus sp. NSP9.1]
TTGTATATCCAAAATCTTACATTATCATCTTACCGTAACTATGAGCGCCTTGAGCTCCAATTTGAAAACAAAGTGAACGT
GATTATTGGAGAAAACGCCCAGGGCAAAACAAATTTGATGGAAGCGATCTATGTGCTTGCGATGGCAAAATCCCATCGAA
CTTCAAATGACAAAGAACTCATACGATGGGATGAAGACTATGCTAAAATAGAAGGCAGGGTCATGAAAAAAAACGGTTCT
GTTCCCATCCAGCTCGTGATTTCCAAAAAAGGAAAAAAAGGCAAGGTCAATCATATTGAGCAGCAAAAGCTGAGCCAGTA
CGTCGGGGCTGTTAACACGATTATGTTTGCACCGGAGGATTTGAACCTTGTAAAAGGAAGCCCTCAGGTCAGAAGAAGGT
TTCTCGATATGGAAATCGGCCAAGTATCACCAGTCTATCTTCATGACCTTTCCCTTTACCAAAAAATCCTTTCACAGCGA
AATCATTTCTTGAAGCAGCTTCAAACGCGAAAACAAACCGATCAAACGATGCTTGACGTGCTGACGGAACAGCTTGCGGA
ATTTGCAGCAAAGGTTGTGATCAAACGCCTTCAGTTTGTCGATCAGCTCGAAAAGTGGGCGCAGCCCATCCACTCCGGAA
TCTCAAGAGGGCTGGAGGAACTGACCTTGAAGTATCATACATCTCTTCACGTATCAGATTCGCCTGATTTGTCGAAAATG
ATCAATAGTTATCAAGAAGCGTTTTCTAAATTGCGAGATAAAGAAATAGACCGGGGAGTATCCCTTTCAGGACCTCACAG
GGATGATGTTCTTTTTTATGTCAATGGACGGGATGTGCAGACTTACGGTTCACAGGGTCAGCAGCGGACGACCGCATTGT
CGCTCAAATTGGCGGAAATCGATTTGATTCAGGAAGAGATTGGGGAATACCCGATTCTGCTTTTGGATGATGTATTATCA
GAGCTGGATGACTACCGGCAGTCCCACCTGCTCCACACCATTCAAGGCCGCGTTCAAACATTCGTAACAACGACAAGCGT
TGATGGAATTGACCATAAAACCTTAAATGAAGCAGCTATCTTTCGTGTAGAAAATGGCACGCTATTGGACTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I7F816

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

89.431

99.73

0.892

Multiple sequence alignment