Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AXA69_RS06345 Genome accession   NZ_CP048223
Coordinates   1312838..1314277 (-) Length   479 a.a.
NCBI ID   WP_005314410.1    Uniprot ID   A4SHX7
Organism   Aeromonas salmonicida subsp. salmonicida strain J223     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1307838..1319277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXA69_RS06315 (AXA69_006300) dagF 1308101..1308841 (+) 741 WP_005314402.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  AXA69_RS06320 (AXA69_006305) - 1308945..1310859 (+) 1915 Protein_1224 BglG family transcription antiterminator -
  AXA69_RS06325 (AXA69_006310) npr 1310878..1311150 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  AXA69_RS06330 (AXA69_006315) rapZ 1311171..1312037 (-) 867 WP_005314405.1 RNase adapter RapZ -
  AXA69_RS06335 (AXA69_006320) ptsN 1312080..1312526 (-) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AXA69_RS06340 (AXA69_006325) hpf 1312529..1312816 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  AXA69_RS06345 (AXA69_006330) rpoN/rpoN1 1312838..1314277 (-) 1440 WP_005314410.1 RNA polymerase factor sigma-54 Machinery gene
  AXA69_RS06350 (AXA69_006335) lptB 1314343..1315068 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  AXA69_RS06355 (AXA69_006340) lptA 1315072..1315599 (-) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  AXA69_RS06360 (AXA69_006345) lptC 1315586..1316146 (-) 561 WP_005314417.1 LPS export ABC transporter periplasmic protein LptC -
  AXA69_RS06365 (AXA69_006350) kdsC 1316143..1316697 (-) 555 WP_005314419.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AXA69_RS06370 (AXA69_006355) - 1316697..1317692 (-) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  AXA69_RS06375 (AXA69_006360) mlaF 1317900..1318700 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53962.92 Da        Isoelectric Point: 4.3718

>NTDB_id=420076 AXA69_RS06345 WP_005314410.1 1312838..1314277(-) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J223]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=420076 AXA69_RS06345 WP_005314410.1 1312838..1314277(-) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J223]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4SHX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484

Multiple sequence alignment