Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GWA24_RS05255 Genome accession   NZ_CP048019
Coordinates   1149640..1151010 (+) Length   456 a.a.
NCBI ID   WP_002832361.1    Uniprot ID   A0AAN5Y9S9
Organism   Pediococcus acidilactici strain CACC 537     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1144640..1156010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWA24_RS05225 (GWA24_05235) - 1145401..1146072 (+) 672 WP_063504432.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  GWA24_RS05235 (GWA24_05245) - 1146466..1147812 (-) 1347 WP_002832366.1 aminopeptidase C -
  GWA24_RS05240 (GWA24_05250) rpiA 1147861..1148547 (-) 687 WP_002832365.1 ribose-5-phosphate isomerase RpiA -
  GWA24_RS05245 (GWA24_05255) - 1148558..1148881 (-) 324 WP_002832363.1 GNAT family N-acetyltransferase -
  GWA24_RS05250 (GWA24_05260) - 1149010..1149546 (+) 537 WP_002832362.1 dUTP diphosphatase -
  GWA24_RS05255 (GWA24_05265) radA 1149640..1151010 (+) 1371 WP_002832361.1 DNA repair protein RadA Machinery gene
  GWA24_RS05260 (GWA24_05270) - 1151126..1152274 (+) 1149 WP_063504431.1 PIN/TRAM domain-containing protein -
  GWA24_RS05265 (GWA24_05275) gltX 1152399..1153885 (+) 1487 Protein_1017 glutamate--tRNA ligase -
  GWA24_RS05270 (GWA24_05280) cysS 1154042..1155460 (+) 1419 WP_024862956.1 cysteine--tRNA ligase -
  GWA24_RS05275 (GWA24_05285) - 1155453..1155866 (+) 414 WP_004165556.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50252.79 Da        Isoelectric Point: 7.4153

>NTDB_id=419468 GWA24_RS05255 WP_002832361.1 1149640..1151010(+) (radA) [Pediococcus acidilactici strain CACC 537]
MAKVKTQFVCNNCGYASPKFLGRCPNCGKWNTLVEERMSDPKAERKSRVSFDGKHTQPQLISDVAMHEEPRVKTGMEELN
RVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAETQRKVLYVSGEESASQIKMRAERLKVNSERFYLYPETDMSSVRA
VIEELHPEYVIIDSVQTMQEPDIESAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLKDASGSAIVVSMEGTRPILVEIQALITPTIFG
NAQRTASGLDRNRVSLIMAVLEKRAKLTLQNQDAYLKAAGGVKLDEPAIDLALAISIVSSFKNQGTRPTDSFVGEIGLTG
EIRRVNRIEQRVAEAQKLGFKRILIPKNNLKGWQPPEGIEVVGVATIADAIRRAFN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=419468 GWA24_RS05255 WP_002832361.1 1149640..1151010(+) (radA) [Pediococcus acidilactici strain CACC 537]
ATGGCAAAAGTTAAGACCCAGTTTGTATGCAATAATTGCGGATACGCCTCCCCTAAATTTTTAGGACGCTGTCCCAATTG
TGGTAAGTGGAACACTTTAGTTGAAGAACGGATGAGCGACCCAAAGGCCGAACGAAAAAGTCGGGTGAGCTTTGATGGCA
AACATACGCAACCACAGTTGATTTCGGATGTTGCGATGCACGAGGAGCCCCGGGTAAAGACCGGGATGGAAGAACTTAAC
CGCGTGCTTGGTGGTGGGGTGGTTGACGGCTCCCTAGTCTTAATTGGTGGGGATCCTGGAATCGGAAAATCAACCCTGTT
GTTACAGCTATCAGGACAGTTGGCAGAGACGCAACGCAAAGTACTTTACGTTTCGGGTGAAGAAAGTGCTTCCCAGATTA
AGATGCGTGCTGAACGCTTGAAAGTTAATAGTGAGCGCTTCTATTTGTATCCAGAGACGGATATGTCTAGCGTGCGGGCG
GTGATTGAGGAGTTACACCCCGAATACGTGATCATTGATTCGGTTCAGACCATGCAGGAACCGGATATTGAATCGGCGGT
GGGGAGCGTTTCTCAAATTCGAGAAATTACCGCAGAATTGATGCAAATCGCGAAAACCAATAACATTACCATCTTCATCG
TTGGTCACGTGACCAAGGGCGGCGCAATTGCGGGCCCTAAGATTTTGGAACACATGGTGGATACGGTGCTTTACTTTGAA
GGTGATCTGCACCACACTTACCGGATCCTGCGGGCGGTGAAGAACCGGTTTGGTTCCACGAACGAATTAGGAATCTTTGA
GATGCGCGAAGAAGGGTTACGCGAGGTGGCTAACCCGTCAGAAATTTTCTTAGAAGAGCGTTTAAAAGATGCTTCGGGAT
CGGCAATTGTAGTTTCAATGGAAGGAACTCGGCCGATTTTAGTTGAAATACAAGCCTTGATCACGCCCACCATCTTCGGC
AATGCGCAGCGCACGGCGAGCGGGTTAGACCGCAACCGAGTTTCGCTGATCATGGCGGTGCTCGAAAAACGGGCAAAGCT
TACTTTGCAAAACCAAGACGCCTATTTAAAGGCGGCGGGCGGCGTCAAGCTTGACGAACCCGCAATTGACTTAGCGTTGG
CGATTAGTATTGTTTCAAGTTTTAAAAATCAGGGAACCCGGCCGACCGATAGTTTTGTTGGTGAAATCGGCTTAACTGGT
GAAATTCGGCGGGTTAACCGAATTGAACAACGGGTCGCTGAAGCACAAAAGCTTGGTTTTAAGCGGATTTTGATTCCAAA
GAATAATTTAAAGGGGTGGCAGCCCCCCGAAGGAATTGAAGTTGTAGGGGTAGCTACGATTGCCGACGCGATTCGGCGGG
CATTTAATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.033

99.781

0.669
  radA Streptococcus mitis NCTC 12261

66.813

99.781

0.667
  radA Streptococcus pneumoniae Rx1

66.813

99.781

0.667
  radA Streptococcus pneumoniae TIGR4

66.813

99.781

0.667
  radA Streptococcus pneumoniae D39

66.813

99.781

0.667
  radA Streptococcus pneumoniae R6

66.813

99.781

0.667
  radA Bacillus subtilis subsp. subtilis str. 168

65.351

100

0.654

Multiple sequence alignment