Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GVU25_RS09985 Genome accession   NZ_CP048014
Coordinates   2080911..2081699 (-) Length   262 a.a.
NCBI ID   WP_371971077.1    Uniprot ID   -
Organism   Acinetobacter towneri strain 205     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2075911..2086699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVU25_RS09965 (GVU25_09920) - 2076936..2077637 (-) 702 WP_004975103.1 YfgM family protein -
  GVU25_RS09970 (GVU25_09925) hisS 2077668..2078963 (-) 1296 WP_004975101.1 histidine--tRNA ligase -
  GVU25_RS09975 (GVU25_09930) ispG 2078967..2080088 (-) 1122 WP_004975100.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GVU25_RS09980 (GVU25_09935) - 2080111..2080920 (-) 810 WP_004975098.1 helix-turn-helix domain-containing protein -
  GVU25_RS09985 (GVU25_09940) pilF 2080911..2081699 (-) 789 WP_371971077.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GVU25_RS09990 (GVU25_09945) rlmN 2081775..2083007 (-) 1233 WP_167846248.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GVU25_RS09995 (GVU25_09950) ndk 2083145..2083576 (-) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  GVU25_RS10000 (GVU25_09955) iscX 2083738..2083938 (-) 201 WP_004975089.1 Fe-S cluster assembly protein IscX -
  GVU25_RS10005 (GVU25_09960) - 2084040..2084591 (-) 552 WP_004975087.1 phosphatase PAP2 family protein -
  GVU25_RS10010 (GVU25_09965) - 2084617..2085900 (-) 1284 WP_167846249.1 glycosyltransferase family 4 protein -
  GVU25_RS10015 (GVU25_09970) - 2086119..2086544 (-) 426 WP_104470588.1 hypothetical protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29451.33 Da        Isoelectric Point: 8.7703

>NTDB_id=419445 GVU25_RS09985 WP_371971077.1 2080911..2081699(-) (pilF) [Acinetobacter towneri strain 205]
MAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSTANMMMGVLLQQE
GSKLNLEKADAYFKRAIAADPKNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDIENAE
KSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVLVNQLRAL
FPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=419445 GVU25_RS09985 WP_371971077.1 2080911..2081699(-) (pilF) [Acinetobacter towneri strain 205]
ATCGCACAAATTACAACTGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCACAGCTCCAACCTTAGGGCAAAA
AGACCCTGAAAAAGCGGTGCAAGTACGTACGCAGTTGGCAGCAGAATACTTAAAAAATGGCGATTTAGACTCAGCCAAAC
GCGCACTTGATCAAGCTTTGGAAAGCGATTCACGTGATTCCACCGCTAATATGATGATGGGCGTGTTGCTGCAACAAGAA
GGCAGCAAGCTGAATTTAGAAAAAGCCGATGCGTATTTTAAGCGTGCCATTGCCGCTGACCCAAAAAATGCACAAGCGCG
TAACAACTATGGTACGTATTTATATCAAGTAGAACGCTATAATGATGCGATTGAGCAGCTCAGTATTGCAGGTGCAACAC
TGGGTTATGATCAACGCTTTAGAGCATTGGAAAATGTAGGTCGTATTTACTTGAAATTAGGCGATATAGAGAATGCCGAA
AAGTCATTCAAACAAGCCTTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGTCGGAAATTTTATATTTAAG
ACAACAATTTGCACCCGCGCGACAATTGTATGAACAATTTGTACGTGCTGTGGGGCAAAAAAATCAGGGTGCGCGTGCCT
TATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTAGTGAACCAGCTGCGTGCATTA
TTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(93-125)

Tetratricopeptide repeat (TPR_16)

(39-76)

Tetratricopeptide repeat (TPR_16)

(147-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.778

96.183

0.748
  pilF Acinetobacter baylyi ADP1

72.047

96.947

0.698

Multiple sequence alignment