Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   GWC79_RS02045 Genome accession   NZ_CP048008
Coordinates   399704..401053 (-) Length   449 a.a.
NCBI ID   WP_228865417.1    Uniprot ID   A0A4Y5MXM0
Organism   Staphylococcus lugdunensis strain CGMH-SL118     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 378357..406224 399704..401053 within 0


Gene organization within MGE regions


Location: 378357..406224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWC79_RS01935 mecA 378357..380366 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  GWC79_RS01940 mecR1 380463..382220 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  GWC79_RS01945 mecI 382220..382591 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  GWC79_RS01950 psm-mec 382676..382744 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  GWC79_RS01955 - 383075..384247 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  GWC79_RS01960 - 384410..385540 (+) 1131 WP_010922808.1 ROK family transcriptional regulator -
  GWC79_RS01965 cstB 385654..386988 (-) 1335 Protein_371 persulfide dioxygenase-sulfurtransferase CstB -
  GWC79_RS01970 - 387020..388084 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  GWC79_RS01975 cstR 388220..388480 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  GWC79_RS01980 - 388480..389118 (+) 639 Protein_374 sulfite exporter TauE/SafE family protein -
  GWC79_RS13750 - 389172..389231 (-) 60 Protein_375 DNA repair protein RadC -
  GWC79_RS01990 - 389463..390125 (-) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  GWC79_RS13755 - 390517..390576 (+) 60 WP_010959190.1 erythromycin resistance leader peptide -
  GWC79_RS01995 erm(A) 390659..391390 (+) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  GWC79_RS02000 - 391516..392298 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  GWC79_RS02005 - 392449..392826 (-) 378 WP_000361059.1 DUF6262 family protein -
  GWC79_RS02010 - 392833..394725 (-) 1893 WP_001557544.1 site-specific integrase -
  GWC79_RS02015 - 394722..395807 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  GWC79_RS02020 - 395926..396243 (-) 318 WP_061641403.1 JAB domain-containing protein -
  GWC79_RS02025 - 396264..396770 (-) 507 WP_001092169.1 DUF1643 domain-containing protein -
  GWC79_RS02030 - 396788..397099 (-) 312 WP_000700853.1 DUF960 family protein -
  GWC79_RS02035 - 397186..397536 (-) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  GWC79_RS02040 ccrB 398054..399682 (-) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  GWC79_RS02045 ccrA 399704..401053 (-) 1350 WP_228865417.1 cassette chromosome recombinase CcrA Machinery gene
  GWC79_RS02050 cch1 401287..403080 (-) 1794 WP_228865422.1 cassette chromosome replicative helicase -
  GWC79_RS02055 ssb 403080..403376 (-) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  GWC79_RS02060 - 403569..404615 (-) 1047 WP_001042023.1 hypothetical protein -
  GWC79_RS02065 - 405070..406224 (+) 1155 WP_000912116.1 ATP-binding protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52619.21 Da        Isoelectric Point: 9.9639

>NTDB_id=419366 GWC79_RS02045 WP_228865417.1 399704..401053(-) (ccrA) [Staphylococcus lugdunensis strain CGMH-SL118]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=419366 GWC79_RS02045 WP_228865417.1 399704..401053(-) (ccrA) [Staphylococcus lugdunensis strain CGMH-SL118]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y5MXM0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

99.332

100

0.993
  ccrA Staphylococcus aureus COL

75.056

100

0.751

Multiple sequence alignment