Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GWK37_RS18050 Genome accession   NZ_CP048002
Coordinates   3703367..3703801 (+) Length   144 a.a.
NCBI ID   WP_021493650.1    Uniprot ID   -
Organism   Bacillus velezensis strain CACC 316     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3698367..3708801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK37_RS18045 (GWK37_18055) - 3701867..3703078 (-) 1212 WP_115940971.1 cytochrome P450 family protein -
  GWK37_RS18050 (GWK37_18060) nucA/comI 3703367..3703801 (+) 435 WP_021493650.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GWK37_RS18055 (GWK37_18065) - 3703861..3704616 (+) 756 WP_003154084.1 YoaK family protein -
  GWK37_RS18060 (GWK37_18070) - 3704650..3705012 (-) 363 WP_007410383.1 hypothetical protein -
  GWK37_RS18065 (GWK37_18075) - 3705205..3706533 (-) 1329 WP_022553314.1 S8 family peptidase -
  GWK37_RS18070 (GWK37_18080) - 3706712..3706945 (+) 234 WP_038461471.1 hypothetical protein -
  GWK37_RS18075 (GWK37_18085) - 3707201..3707908 (+) 708 WP_014417828.1 poly-gamma-glutamate hydrolase family protein -
  GWK37_RS18080 (GWK37_18090) - 3707968..3708420 (+) 453 WP_014305039.1 OsmC family protein -
  GWK37_RS18085 (GWK37_18095) - 3708434..3708787 (-) 354 WP_029973435.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15480.42 Da        Isoelectric Point: 7.2418

>NTDB_id=419269 GWK37_RS18050 WP_021493650.1 3703367..3703801(+) (nucA/comI) [Bacillus velezensis strain CACC 316]
MNAFMKWAASLLLVISLQVGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=419269 GWK37_RS18050 WP_021493650.1 3703367..3703801(+) (nucA/comI) [Bacillus velezensis strain CACC 316]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGGTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment