Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GWK37_RS10945 Genome accession   NZ_CP048002
Coordinates   2195797..2196234 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain CACC 316     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2190797..2201234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK37_RS10920 (GWK37_10925) - 2191817..2192953 (+) 1137 WP_115940419.1 zinc-dependent alcohol dehydrogenase -
  GWK37_RS10925 (GWK37_10930) - 2192968..2193402 (+) 435 WP_115940420.1 RDD family protein -
  GWK37_RS10930 (GWK37_10935) - 2193519..2193797 (+) 279 WP_263612330.1 YckD family protein -
  GWK37_RS10935 (GWK37_10940) - 2193901..2195337 (+) 1437 WP_061861835.1 glycoside hydrolase family 1 protein -
  GWK37_RS10940 (GWK37_10945) nin/comJ 2195378..2195776 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  GWK37_RS10945 (GWK37_10950) nucA/comI 2195797..2196234 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  GWK37_RS10950 (GWK37_10955) hxlB 2196565..2197122 (-) 558 WP_115940421.1 6-phospho-3-hexuloisomerase -
  GWK37_RS10955 (GWK37_10960) hxlA 2197119..2197754 (-) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  GWK37_RS10960 (GWK37_10965) - 2197986..2198348 (+) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=419241 GWK37_RS10945 WP_003156588.1 2195797..2196234(-) (nucA/comI) [Bacillus velezensis strain CACC 316]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=419241 GWK37_RS10945 WP_003156588.1 2195797..2196234(-) (nucA/comI) [Bacillus velezensis strain CACC 316]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment