Detailed information NTG0419155

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GSS20_RS13800
• Genome accession: NZ_CP047995
• Coordinates: 2957635..2958651 (-)
• Length: 338 a.a.
• NCBI ID: WP_031778293.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain 20150710009
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2952635..2963651

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GSS20_RS13775 (GSS20_14465)	aroK	2953495..2954013 (-)	519	WP_005381319.1	shikimate kinase AroK	-
GSS20_RS13780 (GSS20_14470)	pilQ	2954202..2955950 (-)	1749	WP_005488227.1	type IV pilus secretin PilQ	Machinery gene
GSS20_RS13785 (GSS20_14475)	pilP	2955982..2956497 (-)	516	WP_005480477.1	pilus assembly protein PilP	Machinery gene
GSS20_RS13790 (GSS20_14480)	pilO	2956487..2957080 (-)	594	WP_005480482.1	type 4a pilus biogenesis protein PilO	Machinery gene
GSS20_RS13795 (GSS20_14485)	pilN	2957073..2957651 (-)	579	WP_140391938.1	PilN domain-containing protein	Machinery gene
GSS20_RS13800 (GSS20_14490)	pilM	2957635..2958651 (-)	1017	WP_031778293.1	type IV pilus biogenesis protein PilM	Machinery gene
GSS20_RS13805 (GSS20_14495)	-	2958794..2961364 (+)	2571	WP_140222517.1	penicillin-binding protein 1A	-
GSS20_RS13810 (GSS20_14500)	oxyR	2961420..2962328 (-)	909	WP_005465160.1	DNA-binding transcriptional regulator OxyR	-
GSS20_RS13815 (GSS20_14505)	-	2962499..2963227 (+)	729	WP_015297397.1	redoxin family protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37820.32 Da
• Isoelectric Point: 6.6637

>NTDB_id=419155 GSS20_RS13800 WP_031778293.1 2957635..2958651(-) (pilM) [Vibrio parahaemolyticus strain 20150710009]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDDQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide

• Length: 1017 bp

>NTDB_id=419155 GSS20_RS13800 WP_031778293.1 2957635..2958651(-) (pilM) [Vibrio parahaemolyticus strain 20150710009]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGATCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (237-326)

• Type IV pilus assembly protein PilM; (PilM_2) (8-215)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	84.615	100	0.846
pilM	Vibrio cholerae strain A1552	48.368	99.704	0.482