Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GWK28_RS15875 Genome accession   NZ_CP047973
Coordinates   3324316..3325116 (-) Length   266 a.a.
NCBI ID   WP_187142553.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain DETAB-P2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3319316..3330116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK28_RS15855 (GWK28_15875) - 3320381..3321079 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  GWK28_RS15860 (GWK28_15880) hisS 3321108..3322400 (-) 1293 WP_000095253.1 histidine--tRNA ligase -
  GWK28_RS15865 (GWK28_15885) ispG 3322397..3323512 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GWK28_RS15870 (GWK28_15890) - 3323534..3324325 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  GWK28_RS15875 (GWK28_15895) pilF 3324316..3325116 (-) 801 WP_187142553.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GWK28_RS15880 (GWK28_15900) rlmN 3325132..3326364 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GWK28_RS15885 (GWK28_15905) ndk 3326486..3326917 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  GWK28_RS15890 (GWK28_15910) iscX 3327031..3327231 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  GWK28_RS15895 (GWK28_15915) - 3327331..3327882 (-) 552 WP_032014118.1 phosphatase PAP2 family protein -
  GWK28_RS15900 (GWK28_15920) - 3327907..3329205 (-) 1299 WP_085940557.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30080.37 Da        Isoelectric Point: 9.0242

>NTDB_id=418917 GWK28_RS15875 WP_187142553.1 3324316..3325116(-) (pilF) [Acinetobacter baumannii strain DETAB-P2]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSNPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=418917 GWK28_RS15875 WP_187142553.1 3324316..3325116(-) (pilF) [Acinetobacter baumannii strain DETAB-P2]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAATCCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment