Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GWI30_RS19215 Genome accession   NZ_CP047962
Coordinates   4166273..4167712 (-) Length   479 a.a.
NCBI ID   WP_041204880.1    Uniprot ID   -
Organism   Aeromonas media strain MC64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4161273..4172712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWI30_RS19185 (GWI30_19325) degS 4162024..4163145 (+) 1122 WP_041204883.1 outer membrane-stress sensor serine endopeptidase DegS -
  GWI30_RS23870 (GWI30_19330) - 4163248..4164297 (+) 1050 Protein_3736 PTS sugar transporter subunit IIA -
  GWI30_RS19195 (GWI30_19335) npr 4164316..4164588 (-) 273 WP_161507585.1 PTS phosphocarrier protein NPr -
  GWI30_RS19200 (GWI30_19340) rapZ 4164609..4165475 (-) 867 WP_161507586.1 RNase adapter RapZ -
  GWI30_RS19205 (GWI30_19345) ptsN 4165516..4165962 (-) 447 WP_005331355.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GWI30_RS19210 (GWI30_19350) hpf 4165965..4166252 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  GWI30_RS19215 (GWI30_19355) rpoN/rpoN1 4166273..4167712 (-) 1440 WP_041204880.1 RNA polymerase factor sigma-54 Machinery gene
  GWI30_RS19220 (GWI30_19360) lptB 4167778..4168503 (-) 726 WP_161507587.1 LPS export ABC transporter ATP-binding protein -
  GWI30_RS19225 (GWI30_19365) lptA 4168516..4169034 (-) 519 WP_041204879.1 lipopolysaccharide transport periplasmic protein LptA -
  GWI30_RS19230 (GWI30_19370) lptC 4169021..4169581 (-) 561 WP_005331338.1 LPS export ABC transporter periplasmic protein LptC -
  GWI30_RS19235 (GWI30_19375) kdsC 4169578..4170132 (-) 555 WP_005331333.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GWI30_RS19240 (GWI30_19380) - 4170132..4171127 (-) 996 WP_161507588.1 KpsF/GutQ family sugar-phosphate isomerase -
  GWI30_RS19245 (GWI30_19385) mlaF 4171334..4172134 (+) 801 WP_005331323.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53771.74 Da        Isoelectric Point: 4.4457

>NTDB_id=418803 GWI30_RS19215 WP_041204880.1 4166273..4167712(-) (rpoN/rpoN1) [Aeromonas media strain MC64]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAHETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=418803 GWI30_RS19215 WP_041204880.1 4166273..4167712(-) (rpoN/rpoN1) [Aeromonas media strain MC64]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCACGAAACCAGTCCGATAGAACCCCAGGACGCCAGTCAACTCGACTCCAGTGACGCAATGGCACAGGACAAGATGCCG
GATGAACTGCCGGTGGACACTACCTGGGATGAGGTCTATTCCGCCGGCACGGCTCAGAGCGCTGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAATCTGCAGGATTATCTGTTGTGGCAGATGCGACTGACGCCGTTCA
GTGATCTGGATGCCGCGATTGCCCTCGCCATCATCGATGCCATCGATGAGTCTGGCTACCTGACCGTTTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATCGCCGCTCGCTCGGTACAGGAGTGTCTGTTGATCCAGCTTGGCCAGTATTCGCCGGATCTGCCTT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACTTGCTCGGCAACCGTGACTACCGCACCCTGGCGCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTACTGGATCTCATCCAGCAGCTGGAGCCCAGACCCGGTAATGGCATCAT
CCAGAGCGAATCGCAATACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGGGCGAAGTGGGTGGTTGAACTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAACGAGGCCTATGCGGCAATGGCACGCAATGCCCGCAGCAGCACGGATACTCAGTTC
ATTCGTTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGAAACGACACCCTGCTCAAGGTGGCAAG
CTGCATCGTCGAGCAGCAACAGGGTTTCTTCGAGTACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.667

100

0.497

Multiple sequence alignment