Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   SynA1560_RS00100 Genome accession   NZ_CP047933
Coordinates   23241..25022 (-) Length   593 a.a.
NCBI ID   WP_186500823.1    Uniprot ID   -
Organism   Synechococcus sp. A15-60     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18241..30022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SynA1560_RS00080 (SynA1560_00016) msrB 19122..19631 (+) 510 WP_186469760.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SynA1560_RS00085 (SynA1560_00017) - 19666..20856 (+) 1191 WP_370523167.1 NAD(P)/FAD-dependent oxidoreductase -
  SynA1560_RS00090 (SynA1560_00018) - 20867..22141 (-) 1275 WP_186500822.1 type II secretion system F family protein -
  SynA1560_RS00095 (SynA1560_00020) - 22158..23230 (-) 1073 Protein_18 type IV pilus twitching motility protein PilT -
  SynA1560_RS00100 (SynA1560_00021) pilB/pilB1 23241..25022 (-) 1782 WP_186500823.1 GspE/PulE family protein Machinery gene
  SynA1560_RS00105 (SynA1560_00022) grpE 25117..25842 (+) 726 WP_186501514.1 nucleotide exchange factor GrpE -
  SynA1560_RS00110 (SynA1560_00023) dnaJ 25885..27015 (+) 1131 WP_186469764.1 molecular chaperone DnaJ -
  SynA1560_RS00115 (SynA1560_00024) - 27018..27260 (+) 243 WP_186500824.1 sulfurtransferase TusA family protein -
  SynA1560_RS00120 (SynA1560_00025) rsgA 27280..28161 (+) 882 WP_186470905.1 ribosome small subunit-dependent GTPase A -
  SynA1560_RS00125 (SynA1560_00026) - 28136..28477 (-) 342 WP_186469766.1 YbaB/EbfC family nucleoid-associated protein -
  SynA1560_RS00130 (SynA1560_00027) murB 28502..29434 (-) 933 WP_186500825.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 593 a.a.        Molecular weight: 64524.03 Da        Isoelectric Point: 4.7342

>NTDB_id=418529 SynA1560_RS00100 WP_186500823.1 23241..25022(-) (pilB/pilB1) [Synechococcus sp. A15-60]
MIDDRRRLELELLLQQSIPGNLGRADAPSLLQRLKTMGFNPARHAALSEQLTALLDLQSSPLPDHSLGQATPAPTLAINA
SEPIEPSPDPDAEETTPAVNNMEANTGATNTGANNTVAETATSYLQEFSVDGVLEQDPEEAAAESAGPEDLESSLNDADR
SPVINLVDRILMQALQLGASDIHVEPQQAGLQLRFRQDGVLQSHIEPLPSRLIPAVTSRFKIMADLDIAERRIAQDGRIR
RRFQNRTVDFRVNSLPSRYGEKIVLRLLDSSATQLGLDKLISNPEALELVRALGSKPFGMILVTGPTGSGKSTTLYSLLA
ERNEPGINISTVEDPIEYTLPGITQCQVNRDKGFDFSQALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLH
CNDAPSAIARLDEMGVEPFMVSASLIGIVSQRLLRRVCPHCRIAYRPDPEELARFGLMASHEADVSFFKANHQDIEANNC
PHCQGSGYKGRVGVYEVLRMNEELAAAVAKGATTDMVRQLALESGMKTLLGYSLDLVREGHTTLEEVGRMVLTDAGLESE
RRARALSSLTCSGCGGGLQEGWLECPYCLTPRH

Nucleotide


Download         Length: 1782 bp        

>NTDB_id=418529 SynA1560_RS00100 WP_186500823.1 23241..25022(-) (pilB/pilB1) [Synechococcus sp. A15-60]
GTGATCGACGATCGCCGGCGTCTCGAACTTGAATTGCTGTTGCAGCAGTCGATCCCGGGAAATCTCGGCCGTGCCGATGC
GCCATCCCTGCTGCAGAGGCTGAAAACCATGGGCTTCAATCCAGCGCGGCATGCCGCGCTGAGCGAACAGCTGACGGCAC
TGTTAGATCTGCAATCCTCCCCTCTTCCAGACCACTCGTTGGGCCAGGCCACCCCTGCACCAACGCTGGCAATCAATGCA
AGTGAACCGATCGAGCCTTCACCTGATCCAGACGCGGAAGAGACCACCCCTGCGGTGAACAACATGGAGGCCAACACCGG
CGCGACGAACACCGGTGCAAACAACACCGTGGCGGAGACCGCCACCTCCTATCTACAGGAGTTCTCCGTGGACGGCGTGC
TGGAGCAGGACCCGGAGGAGGCAGCAGCGGAATCAGCCGGTCCGGAGGATCTGGAGTCAAGCCTCAACGATGCGGACCGT
TCGCCGGTGATCAACCTGGTGGACCGGATCCTGATGCAGGCCCTTCAACTGGGAGCCAGTGACATTCACGTAGAGCCCCA
GCAGGCAGGTCTGCAACTGCGCTTCCGTCAGGACGGTGTGCTGCAAAGCCACATTGAACCCCTGCCCAGCCGGCTGATCC
CAGCCGTGACCTCCCGCTTCAAAATCATGGCGGACCTGGACATCGCCGAGCGCCGCATCGCCCAGGACGGTCGGATCCGC
CGTCGTTTCCAAAACCGCACAGTGGACTTCCGGGTGAACAGCCTGCCCAGCCGGTACGGCGAAAAGATTGTGCTGCGACT
ACTGGACAGCTCCGCCACCCAGCTGGGTCTCGACAAGCTGATCAGCAACCCGGAGGCACTGGAATTGGTGCGAGCCCTCG
GGTCCAAACCGTTCGGAATGATCCTGGTGACCGGTCCGACGGGATCCGGTAAATCCACCACTCTCTATTCGTTGCTGGCG
GAACGCAACGAGCCAGGAATCAATATCTCCACGGTTGAAGATCCGATCGAATACACCTTGCCCGGGATCACCCAATGCCA
GGTGAACCGAGATAAAGGGTTCGATTTCAGTCAGGCCCTTCGGGCCTTCATGCGTCAGGACCCGGACGTGCTGCTGGTGG
GCGAAACCCGCGATCTGGAAACAGCGAAAACAGCCATTGAAGCGGCCCTCACCGGTCATTTGGTCTTGACCACCCTGCAT
TGCAACGACGCCCCCAGCGCGATTGCTCGTCTGGATGAGATGGGAGTCGAACCATTCATGGTTAGCGCCTCCCTGATCGG
GATCGTTTCCCAGCGACTGCTGAGGAGGGTCTGCCCTCATTGCCGCATCGCCTATCGACCGGATCCTGAAGAACTAGCGC
GGTTCGGATTGATGGCCAGCCATGAAGCTGACGTGAGCTTTTTCAAGGCCAATCACCAAGACATAGAGGCCAACAACTGC
CCTCACTGTCAGGGCAGCGGTTACAAGGGCCGGGTTGGGGTCTACGAGGTGTTGCGCATGAACGAAGAGCTTGCGGCAGC
CGTAGCCAAAGGCGCCACCACCGACATGGTGCGGCAACTGGCTCTGGAAAGCGGCATGAAAACCCTGCTGGGGTACAGCC
TCGATCTGGTACGGGAGGGCCACACCACCCTGGAGGAGGTGGGGCGCATGGTGCTTACCGACGCGGGCCTGGAATCGGAA
CGGCGCGCCCGGGCGCTGAGCTCACTCACCTGCAGTGGATGTGGTGGCGGGCTCCAGGAAGGTTGGCTGGAATGCCCTTA
CTGCCTCACACCACGTCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(170-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.934

76.728

0.506

Multiple sequence alignment