Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   GWG03_RS09770 Genome accession   NZ_CP047924
Coordinates   1982129..1983757 (+) Length   542 a.a.
NCBI ID   WP_103147903.1    Uniprot ID   -
Organism   Staphylococcus aureus strain SR130     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 1975587..2005566 1982129..1983757 within 0
IScluster/Tn 1984713..1995066 1982129..1983757 flank 956


Gene organization within MGE regions


Location: 1975587..2005566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWG03_RS09745 (GWG03_09770) - 1975587..1976741 (-) 1155 WP_000912116.1 ATP-binding protein -
  GWG03_RS09750 (GWG03_09775) - 1977196..1978242 (+) 1047 WP_001042023.1 hypothetical protein -
  GWG03_RS09755 (GWG03_09780) - 1978435..1978731 (+) 297 WP_000273823.1 single-stranded DNA-binding protein -
  GWG03_RS09760 (GWG03_09785) - 1978731..1980524 (+) 1794 WP_000079473.1 DUF927 domain-containing protein -
  GWG03_RS09765 (GWG03_09790) ccrA 1980758..1982107 (+) 1350 WP_000815638.1 recombinase family protein Machinery gene
  GWG03_RS09770 (GWG03_09795) ccrB 1982129..1983757 (+) 1629 WP_103147903.1 recombinase family protein Machinery gene
  GWG03_RS09775 (GWG03_09800) - 1984123..1984554 (-) 432 Protein_1900 hypothetical protein -
  GWG03_RS09780 (GWG03_09805) - 1984713..1986317 (+) 1605 WP_103147904.1 IS1182-like element IS1182 family transposase -
  GWG03_RS09785 (GWG03_09815) - 1987038..1987697 (-) 660 WP_000802831.1 RibD family protein -
  GWG03_RS09790 (GWG03_09820) - 1987808..1988164 (-) 357 WP_000374054.1 WYL domain-containing protein -
  GWG03_RS09795 (GWG03_09830) - 1988188..1988760 (-) 573 WP_001079938.1 HTH domain-containing protein -
  GWG03_RS09800 (GWG03_09840) aph(3')-IIIa 1989036..1989830 (-) 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
  GWG03_RS09805 (GWG03_09845) sat4 1989923..1990465 (-) 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
  GWG03_RS09810 (GWG03_09850) ant(6) 1990462..1991370 (-) 909 WP_212556254.1 aminoglycoside 6-adenylyltransferase -
  GWG03_RS09815 (GWG03_09855) - 1991403..1992137 (-) 735 WP_000662263.1 class I SAM-dependent methyltransferase -
  GWG03_RS09820 (GWG03_09860) - 1992118..1992987 (-) 870 WP_212556255.1 nucleotidyltransferase domain-containing protein -
  GWG03_RS09825 (GWG03_09865) - 1993090..1993335 (-) 246 WP_000567888.1 single-stranded DNA-binding protein -
  GWG03_RS09830 (GWG03_09870) - 1993462..1995066 (-) 1605 WP_103147904.1 IS1182-like element IS1182 family transposase -
  GWG03_RS09835 (GWG03_09875) - 1995303..1995884 (-) 582 WP_001124173.1 TetR family transcriptional regulator C-terminal domain-containing protein -
  GWG03_RS09840 (GWG03_09880) - 1996090..1996683 (+) 594 WP_000690628.1 recombinase family protein -
  GWG03_RS09845 (GWG03_09885) - 1996655..1998097 (+) 1443 WP_103147960.1 Mu transposase C-terminal domain-containing protein -
  GWG03_RS09850 (GWG03_09890) - 1998090..1998905 (+) 816 WP_000164963.1 AAA family ATPase -
  GWG03_RS09855 (GWG03_09895) - 1999322..1999672 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  GWG03_RS09860 (GWG03_09905) - 1999759..2000070 (+) 312 WP_000700851.1 DUF960 family protein -
  GWG03_RS09865 (GWG03_09910) - 2000085..2000591 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  GWG03_RS09870 (GWG03_09915) - 2000643..2002251 (+) 1609 Protein_1919 IS1182 family transposase -
  GWG03_RS09875 (GWG03_09920) - 2002242..2002502 (-) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  GWG03_RS09880 (GWG03_09925) - 2002474..2003460 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  GWG03_RS09885 (GWG03_09930) mecA 2003557..2005566 (+) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62653.89 Da        Isoelectric Point: 9.8715

>NTDB_id=418472 GWG03_RS09770 WP_103147903.1 1982129..1983757(+) (ccrB) [Staphylococcus aureus strain SR130]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVKNVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=418472 GWG03_RS09770 WP_103147903.1 1982129..1983757(+) (ccrB) [Staphylococcus aureus strain SR130]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCAAGAACGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTGCTATGCCAAAGGACATGGCTATCGTAAAATTGCCAATGCATTGAATCACAAAGGATATGTCACTAAAA
AGGGGAAACCTTTCAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAACACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Recombinase (Recombinase)

(180-280)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.708

100

0.987

Multiple sequence alignment