Detailed information NTG0418350

Bioinformatically predicted

Overview

• Name: sepM
• Type: Regulator
• Locus tag: GV831_RS10360
• Genome accession: NZ_CP047891
• Coordinates: 2092257..2093285 (+)
• Length: 342 a.a.
• NCBI ID: WP_003132657.1
• Uniprot ID: S6FVM6
• Organism: Lactococcus lactis subsp. lactis strain WHH2311
• Function: processing of CSP (predicted from homology) Competence regulation

Genomic Context

Location: 2087257..2098285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GV831_RS10335 (GV831_10295)	-	2087805..2088299 (-)	495	WP_012898649.1	RNA polymerase sigma factor	-
GV831_RS10340 (GV831_10300)	lmrP	2088487..2089711 (+)	1225	Protein_1999	multidrug efflux MFS transporter LmrP	-
GV831_RS10345 (GV831_10305)	-	2089795..2091015 (+)	1221	WP_003132654.1	MFS transporter	-
GV831_RS10350 (GV831_10310)	rsmD	2091186..2091725 (+)	540	WP_003132655.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
GV831_RS10355 (GV831_10315)	coaD	2091773..2092270 (+)	498	WP_010906348.1	pantetheine-phosphate adenylyltransferase	-
GV831_RS10360 (GV831_10320)	sepM	2092257..2093285 (+)	1029	WP_003132657.1	SepM family pheromone-processing serine protease	Regulator
GV831_RS10365 (GV831_10325)	-	2093406..2094263 (+)	858	WP_003132658.1	Rgg/GadR/MutR family transcriptional regulator	-
GV831_RS10370 (GV831_10330)	-	2094447..2096489 (+)	2043	WP_228763148.1	radical SAM protein	-
GV831_RS10375 (GV831_10335)	-	2096482..2097309 (+)	828	WP_014570829.1	radical SAM protein	-
GV831_RS10380 (GV831_10340)	-	2097306..2097515 (+)	210	WP_003132661.1	hypothetical protein	-
GV831_RS10385 (GV831_10345)	-	2097502..2097999 (+)	498	WP_003132662.1	nitroreductase family protein	-

Sequence

Protein

• Length: 342 a.a.
• Molecular weight: 37349.96 Da
• Isoelectric Point: 9.9183

>NTDB_id=418350 GV831_RS10360 WP_003132657.1 2092257..2093285(+) (sepM) [Lactococcus lactis subsp. lactis strain WHH2311]
MKKNKKINPKLKWGISIGLIVVALLALFYPTSYYVEMPGTTEPLGKMVKVEGKKDEHKGDFFLTTVQIARANLATMIYSH
FNSFTSIYSEQEMTGGLNDAQFNRVNQFYMETAQNTAIYQAFKLANKPYELKYEGVYVLDIAKNSTFKNKLELADTITAV
NGQQFTSSADMIAYVSKQKVGDSVTIEYTRIDGTKHKSTGKYIKIANGKTGIGISLVDHTEVVTTPKVTVNAGSIGGPSA
GMMFTLEIYSQLTGKDLRNGREIAGTGTIEHDGSIGQIGGVDKKVATASKEGAKVFLVPDSGTKKESSNNYLGAKAAAKK
LKTKMKIVPVKTIQDALDYLEK

Nucleotide

• Length: 1029 bp

>NTDB_id=418350 GV831_RS10360 WP_003132657.1 2092257..2093285(+) (sepM) [Lactococcus lactis subsp. lactis strain WHH2311]
ATGAAAAAAAATAAAAAAATTAATCCCAAATTAAAATGGGGAATCTCGATTGGTCTAATTGTTGTTGCCCTTTTAGCTTT
ATTTTATCCAACAAGTTACTATGTGGAAATGCCCGGAACAACCGAGCCTTTAGGAAAAATGGTCAAAGTTGAAGGCAAAA
AAGATGAACATAAAGGTGATTTTTTCCTGACGACTGTCCAAATTGCACGAGCAAATCTTGCTACAATGATTTACAGCCAT
TTTAATAGTTTTACAAGCATTTACAGTGAGCAAGAAATGACTGGTGGACTTAACGACGCACAGTTCAACCGTGTCAACCA
GTTTTACATGGAAACGGCACAGAATACAGCGATTTATCAAGCTTTTAAATTAGCTAATAAACCTTATGAACTTAAATATG
AAGGCGTTTATGTCCTTGATATTGCCAAAAATTCGACCTTTAAAAATAAGCTAGAACTTGCAGATACGATTACAGCAGTC
AATGGTCAACAGTTTACATCAAGTGCCGACATGATTGCTTATGTTTCCAAACAAAAAGTCGGTGATTCAGTAACGATTGA
ATACACGAGAATTGACGGCACAAAGCACAAGTCAACTGGCAAATACATCAAAATTGCTAATGGAAAAACAGGAATTGGGA
TCAGCCTAGTTGACCATACTGAAGTCGTGACGACTCCAAAAGTTACTGTCAACGCGGGTTCAATCGGTGGTCCCTCTGCG
GGGATGATGTTTACACTAGAAATTTACAGTCAGTTGACAGGAAAAGATTTGCGAAACGGTCGCGAAATTGCTGGAACTGG
GACGATTGAACATGACGGAAGCATTGGCCAAATCGGCGGTGTTGATAAAAAAGTAGCCACAGCGAGCAAAGAAGGGGCAA
AGGTCTTCTTGGTGCCTGACTCAGGCACCAAAAAAGAAAGTAGCAACAATTACCTTGGAGCCAAAGCCGCCGCTAAAAAA
CTAAAAACGAAGATGAAAATCGTTCCCGTCAAAACTATTCAGGACGCTTTAGATTATTTAGAAAAATAA

Domains

Predicted by InterproScan.

• PDZ domain (PDZ_2) (134-194)

• Lon protease (S16) C-terminal proteolytic domain (Lon_C) (227-338)

3D structure

• AlphaFold DB: S6FVM6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sepM	Streptococcus mutans UA159	48.696	100	0.491