Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   H9195_RS04215 Genome accession   NZ_CP060953
Coordinates   816870..818297 (+) Length   475 a.a.
NCBI ID   WP_001538915.1    Uniprot ID   -
Organism   Escherichia coli strain EC8     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 811870..823297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9195_RS04185 (H9195_04135) pduU 812249..812599 (+) 351 WP_000211397.1 propanediol utilization microcompartment protein PduU -
  H9195_RS04190 (H9195_04140) - 812604..813047 (+) 444 WP_001189443.1 EutP/PduV family microcompartment system protein -
  H9195_RS04195 (H9195_04145) tmaR 813147..813476 (-) 330 WP_000450409.1 DUF496 family protein -
  H9195_RS04200 (H9195_04150) yeeA 813647..814705 (-) 1059 WP_001200890.1 FUSC family protein -
  H9195_RS04205 (H9195_04155) sbmC 814903..815376 (-) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  H9195_RS04210 (H9195_04160) dacD 815495..816661 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  H9195_RS04215 (H9195_04165) sbcB 816870..818297 (+) 1428 WP_001538915.1 exodeoxyribonuclease I Machinery gene
  H9195_RS04220 (H9195_04170) plaP 818371..819729 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  H9195_RS04225 (H9195_04175) yoeI 819719..819781 (-) 63 WP_010723108.1 membrane protein YoeI -
  H9195_RS04230 (H9195_04180) yeeY 819996..820925 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  H9195_RS04235 (H9195_04185) yeeZ 820971..821795 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  H9195_RS04240 (H9195_04190) yoeB 821878..822132 (-) 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  H9195_RS04245 (H9195_04195) yefM 822129..822380 (-) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  H9195_RS04250 (H9195_04200) hisL 822663..822713 (+) 51 WP_001364200.1 his operon leader peptide -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54483.79 Da        Isoelectric Point: 5.4720

>NTDB_id=418317 H9195_RS04215 WP_001538915.1 816870..818297(+) (sbcB) [Escherichia coli strain EC8]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTTWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=418317 H9195_RS04215 WP_001538915.1 816870..818297(+) (sbcB) [Escherichia coli strain EC8]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCACCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGAGAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581

Multiple sequence alignment