Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   M594_RS01400 Genome accession   NZ_CP047883
Coordinates   270445..271269 (+) Length   274 a.a.
NCBI ID   WP_000359015.1    Uniprot ID   A0A6M9F3T7
Organism   Streptococcus mitis strain S022-V3-A4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 265445..276269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M594_RS01365 (M594_01390) asnA 265535..266527 (+) 993 WP_084947345.1 aspartate--ammonia ligase -
  M594_RS01370 (M594_01395) rsmD 266592..267131 (+) 540 WP_173875770.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  M594_RS01375 (M594_01400) coaD 267121..267609 (+) 489 WP_173875771.1 pantetheine-phosphate adenylyltransferase -
  M594_RS01380 (M594_01405) sepM 267593..268630 (+) 1038 WP_033687316.1 SepM family pheromone-processing serine protease Regulator
  M594_RS01385 (M594_01410) - 268688..268918 (+) 231 WP_000250399.1 DUF1146 family protein -
  M594_RS01390 (M594_01415) murA 268942..270225 (+) 1284 WP_173875772.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  M594_RS01395 (M594_01420) - 270218..270406 (+) 189 WP_004241151.1 DNA-directed RNA polymerase subunit beta -
  M594_RS01400 (M594_01425) endA 270445..271269 (+) 825 WP_000359015.1 DNA-entry nuclease EndA Machinery gene
  M594_RS01405 (M594_01430) - 271537..272868 (+) 1332 WP_173875773.1 hemolysin family protein -
  M594_RS01415 (M594_01440) amiA 273544..275523 (+) 1980 WP_173875774.1 peptide ABC transporter substrate-binding protein Regulator

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29950.55 Da        Isoelectric Point: 9.7812

>NTDB_id=418237 M594_RS01400 WP_000359015.1 270445..271269(+) (endA) [Streptococcus mitis strain S022-V3-A4]
MDKKTRQTLIGLLVLLLLSAGSYYIKQMQSTPNSPKTKLSQKKQASEAPSQELAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEDSTGQNYYESMVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=418237 M594_RS01400 WP_000359015.1 270445..271269(+) (endA) [Streptococcus mitis strain S022-V3-A4]
ATGGATAAAAAAACAAGACAGACACTGATCGGACTGCTAGTGTTATTGCTTTTATCTGCGGGAAGCTATTATATCAAGCA
GATGCAATCGACACCAAATAGTCCCAAAACCAAGCTTAGTCAGAAAAAACAAGCGTCTGAAGCCCCTAGTCAAGAATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAAGGGAGCCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCTTACGCTGATAATAAAACAAAGACAGTGGGCAAGGA
AACAGTGCCAACCGTAGCAAATGCCCTCTTGTCTAAGGCTACTCGTCAGTACAAGAATCGTGAAGAAACTGGGAATGGTT
CGACTTCATGGACTCCTCCAGGCTGGCATCAGGTCAAGAATCTAAAGGGTTCTTATACCCATGCGGTCGATAGAGGTCAC
TTGTTAGGCTATGCCTTAATCGGTGGCTTGGATGGTTTTGATGCCTCGACAAGCAATCCTAAAAACATTGCTGTTCAAAC
AGCTTGGGCAAATCAGGCACAGGCCGAGGATTCGACTGGTCAAAACTACTATGAAAGTATGGTACGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACTCTTTATTATGCTTCAAACGAGGATTTAGTTCCTTCAGCTTCACAGATTGAA
GCCAAGTCTTCAGACGGAGAATTGGAATTTAATGTTCTAGTTCCCAATGTTCAAAAAGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACGGTTACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M9F3T7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

96.715

100

0.967
  endA Streptococcus pneumoniae D39

96.715

100

0.967
  endA Streptococcus pneumoniae R6

96.715

100

0.967
  endA Streptococcus pneumoniae TIGR4

96.715

100

0.967

Multiple sequence alignment