Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   H9199_RS10595 Genome accession   NZ_CP060933
Coordinates   2161604..2163031 (-) Length   475 a.a.
NCBI ID   WP_001374015.1    Uniprot ID   -
Organism   Escherichia coli strain EC12     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2156604..2168031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9199_RS10560 (H9199_10465) hisL 2157188..2157238 (-) 51 WP_001364200.1 his operon leader peptide -
  H9199_RS10565 (H9199_10470) yefM 2157521..2157772 (+) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  H9199_RS10570 (H9199_10475) yoeB 2157769..2158023 (+) 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  H9199_RS10575 (H9199_10480) yeeZ 2158106..2158930 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  H9199_RS10580 (H9199_10485) yeeY 2158976..2159905 (+) 930 WP_001374016.1 LysR substrate-binding domain-containing protein -
  H9199_RS10585 (H9199_10490) yoeI 2160120..2160182 (+) 63 WP_010723108.1 membrane protein YoeI -
  H9199_RS10590 (H9199_10495) plaP 2160172..2161530 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  H9199_RS10595 (H9199_10500) sbcB 2161604..2163031 (-) 1428 WP_001374015.1 exodeoxyribonuclease I Machinery gene
  H9199_RS10600 (H9199_10505) dacD 2163240..2164406 (+) 1167 WP_001374019.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  H9199_RS10605 (H9199_10510) sbmC 2164525..2164998 (+) 474 WP_001374018.1 DNA gyrase inhibitor SbmC -
  H9199_RS10610 (H9199_10515) yeeA 2165196..2166254 (+) 1059 WP_001200890.1 FUSC family protein -
  H9199_RS10615 (H9199_10520) tmaR 2166426..2166755 (+) 330 WP_001374017.1 DUF496 family protein -
  H9199_RS10620 (H9199_10525) - 2166856..2167041 (-) 186 Protein_2070 EutP/PduV family microcompartment system protein -
  H9199_RS10625 (H9199_10530) - 2167092..2167238 (+) 147 Protein_2071 transposase domain-containing protein -
  H9199_RS10630 (H9199_10535) - 2167527..2167607 (-) 81 Protein_2072 hypothetical protein -
  H9199_RS10635 (H9199_10540) yeeW 2167653..2167847 (-) 195 WP_000988600.1 DUF5983 family protein -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54496.83 Da        Isoelectric Point: 5.6070

>NTDB_id=418024 H9199_RS10595 WP_001374015.1 2161604..2163031(-) (sbcB) [Escherichia coli strain EC12]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELNSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQVYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=418024 H9199_RS10595 WP_001374015.1 2161604..2163031(-) (sbcB) [Escherichia coli strain EC12]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGAATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGTCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.511

98.947

0.579