Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H9206_RS01965 Genome accession   NZ_CP060899
Coordinates   422126..422593 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain EC21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417126..427593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9206_RS01940 (H9206_01900) rpsG 417267..417737 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  H9206_RS01945 (H9206_01905) fusA 417834..419948 (+) 2115 WP_000124700.1 elongation factor G -
  H9206_RS01950 (H9206_01910) tuf 420019..421203 (+) 1185 WP_000031783.1 elongation factor Tu -
  H9206_RS01955 (H9206_01915) bfd 421386..421580 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  H9206_RS01960 (H9206_01920) bfr 421653..422129 (+) 477 WP_000675515.1 bacterioferritin -
  H9206_RS01965 (H9206_01925) pilD 422126..422593 (-) 468 WP_000340171.1 A24 family peptidase Machinery gene
  H9206_RS01970 (H9206_01930) rpsJ 422972..423283 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  H9206_RS01975 (H9206_01935) rplC 423316..423945 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  H9206_RS01980 (H9206_01940) rplD 423956..424561 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  H9206_RS01985 (H9206_01945) rplW 424558..424860 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  H9206_RS01990 (H9206_01950) rplB 424878..425699 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  H9206_RS01995 (H9206_01955) rpsS 425716..425994 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  H9206_RS02000 (H9206_01960) rplV 426009..426341 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  H9206_RS02005 (H9206_01965) rpsC 426359..427060 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  H9206_RS02010 (H9206_01970) rplP 427073..427483 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=417429 H9206_RS01965 WP_000340171.1 422126..422593(-) (pilD) [Escherichia coli strain EC21]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=417429 H9206_RS01965 WP_000340171.1 422126..422593(-) (pilD) [Escherichia coli strain EC21]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361

Multiple sequence alignment