Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   H9209_RS00335 Genome accession   NZ_CP060880
Coordinates   70085..70753 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EC26     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 65085..75753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9209_RS00305 (H9209_00295) yicC 65104..65967 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  H9209_RS00310 (H9209_00300) rph 66094..66810 (+) 717 WP_001247087.1 ribonuclease PH -
  H9209_RS00315 (H9209_00305) pyrE 66876..67517 (+) 642 WP_000806158.1 orotate phosphoribosyltransferase -
  H9209_RS00320 (H9209_00310) slmA 67554..68150 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  H9209_RS00325 (H9209_00315) dut 68257..68715 (-) 459 WP_000976064.1 dUTP diphosphatase -
  H9209_RS00330 (H9209_00320) coaBC 68693..69913 (-) 1221 WP_000050140.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  H9209_RS00335 (H9209_00325) radC 70085..70753 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  H9209_RS00340 (H9209_00330) rpmB 70970..71206 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  H9209_RS00345 (H9209_00335) rpmG 71227..71394 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  H9209_RS00350 (H9209_00340) mutM 71492..72301 (+) 810 WP_001114549.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  H9209_RS00355 (H9209_00345) coaD 72340..72819 (-) 480 WP_001171862.1 pantetheine-phosphate adenylyltransferase -
  H9209_RS00360 (H9209_00350) waaA 72827..74104 (-) 1278 WP_039268496.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  H9209_RS00365 (H9209_00355) rfaQ 74517..75575 (+) 1059 WP_000360405.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=417177 H9209_RS00335 WP_001297375.1 70085..70753(+) (radC) [Escherichia coli strain EC26]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=417177 H9209_RS00335 WP_001297375.1 70085..70753(+) (radC) [Escherichia coli strain EC26]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAGTTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
CACCGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment