Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   E3S70_RS08295 Genome accession   NZ_CP047801
Coordinates   1637183..1638547 (-) Length   454 a.a.
NCBI ID   WP_160176517.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UP_248     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1632183..1643547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S70_RS08280 (E3S70_08280) cysE 1633003..1633650 (-) 648 WP_001805237.1 serine O-acetyltransferase -
  E3S70_RS08285 (E3S70_08285) gltX 1634074..1635528 (-) 1455 WP_001283787.1 glutamate--tRNA ligase -
  E3S70_RS08290 (E3S70_08290) - 1636085..1637158 (-) 1074 WP_001028366.1 PIN/TRAM domain-containing protein -
  E3S70_RS08295 (E3S70_08295) radA 1637183..1638547 (-) 1365 WP_160176517.1 DNA repair protein RadA Machinery gene
  E3S70_RS08300 (E3S70_08300) - 1639031..1641487 (-) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  E3S70_RS08305 (E3S70_08305) - 1641501..1642508 (-) 1008 WP_000149503.1 protein arginine kinase -
  E3S70_RS08310 (E3S70_08310) - 1642498..1643064 (-) 567 WP_000882071.1 UvrB/UvrC motif-containing protein -
  E3S70_RS08315 (E3S70_08315) - 1643083..1643544 (-) 462 WP_000551762.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49879.45 Da        Isoelectric Point: 7.0049

>NTDB_id=416765 E3S70_RS08295 WP_160176517.1 1637183..1638547(-) (radA) [Staphylococcus aureus strain UP_248]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELVGKVQKLNSIKHETTLRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=416765 E3S70_RS08295 WP_160176517.1 1637183..1638547(-) (radA) [Staphylococcus aureus strain UP_248]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGTAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCTGAGAGTGTTAACAGATTCAGCAGAATTTAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAGGAACAACCTGATTTACTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTACTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACTAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCACAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACAGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCGTTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

AAA domain (AAA_25)

(75-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.674
  radA Streptococcus pneumoniae Rx1

62.054

98.678

0.612
  radA Streptococcus pneumoniae D39

62.054

98.678

0.612
  radA Streptococcus pneumoniae R6

62.054

98.678

0.612
  radA Streptococcus pneumoniae TIGR4

62.054

98.678

0.612
  radA Streptococcus mitis NCTC 12261

62.054

98.678

0.612
  radA Streptococcus mitis SK321

62.054

98.678

0.612

Multiple sequence alignment