Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   H9W85_RS26960 Genome accession   NZ_CP060703
Coordinates   5850299..5850895 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain NRD619     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5845299..5855895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9W85_RS26940 (H9W85_26940) aroK 5846430..5846948 (-) 519 WP_003095833.1 shikimate kinase AroK -
  H9W85_RS26945 (H9W85_26945) pilQ 5846960..5849104 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  H9W85_RS26950 (H9W85_26950) pilP 5849158..5849682 (-) 525 WP_033983631.1 type 4a pilus biogenesis lipoprotein PilP -
  H9W85_RS26955 (H9W85_26955) pilO 5849679..5850302 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  H9W85_RS26960 (H9W85_26960) pilN 5850299..5850895 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  H9W85_RS26965 (H9W85_26965) pilM 5850895..5851959 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  H9W85_RS26970 (H9W85_26970) - 5852144..5854615 (+) 2472 WP_043088268.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=416677 H9W85_RS26960 WP_003095839.1 5850299..5850895(-) (pilN) [Pseudomonas aeruginosa strain NRD619]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=416677 H9W85_RS26960 WP_003095839.1 5850299..5850895(-) (pilN) [Pseudomonas aeruginosa strain NRD619]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369