Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NIT79A3_RS04465 Genome accession   NC_015731
Coordinates   990038..991486 (+) Length   482 a.a.
NCBI ID   WP_013965064.1    Uniprot ID   F8GLM3
Organism   Nitrosomonas sp. Is79A3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 985038..996486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIT79A3_RS04435 (Nit79A3_0894) clsB 985361..986533 (-) 1173 WP_013965059.1 cardiolipin synthase ClsB -
  NIT79A3_RS04440 (Nit79A3_0895) nadA 986655..987752 (-) 1098 WP_013965060.1 quinolinate synthase NadA -
  NIT79A3_RS04450 (Nit79A3_0896) lptC 988123..988689 (+) 567 WP_013965061.1 LPS export ABC transporter periplasmic protein LptC -
  NIT79A3_RS04455 (Nit79A3_0897) lptA 988686..989216 (+) 531 WP_013965062.1 lipopolysaccharide transport periplasmic protein LptA -
  NIT79A3_RS04460 (Nit79A3_0898) lptB 989261..989983 (+) 723 WP_013965063.1 LPS export ABC transporter ATP-binding protein -
  NIT79A3_RS04465 (Nit79A3_0899) rpoN/rpoN1 990038..991486 (+) 1449 WP_013965064.1 RNA polymerase factor sigma-54 Machinery gene
  NIT79A3_RS04470 (Nit79A3_0900) raiA 991506..991835 (+) 330 WP_013965065.1 ribosome-associated translation inhibitor RaiA -
  NIT79A3_RS04475 (Nit79A3_0901) - 991959..992426 (+) 468 WP_013965066.1 PTS sugar transporter subunit IIA -
  NIT79A3_RS04480 (Nit79A3_0902) hprK 992413..993372 (+) 960 WP_013965067.1 HPr(Ser) kinase/phosphatase -
  NIT79A3_RS04485 (Nit79A3_0903) - 993444..994058 (+) 615 WP_013965068.1 flavin prenyltransferase UbiX -
  NIT79A3_RS04490 (Nit79A3_0904) - 994275..994892 (+) 618 WP_013965069.1 hypothetical protein -
  NIT79A3_RS04495 (Nit79A3_0905) - 995025..995627 (-) 603 WP_348225724.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 54883.22 Da        Isoelectric Point: 4.8379

>NTDB_id=41644 NIT79A3_RS04465 WP_013965064.1 990038..991486(+) (rpoN/rpoN1) [Nitrosomonas sp. Is79A3]
MKPTLQLKLSQQLRLTPQLQQSIRLLQLSTLELNQEIERIVQENPLLELREDWNAPVAENTFEHPEFSDDVADFDSVPPE
AAVSEVQAESEENFESDSEWPQENIFSHGTYEDDDHEAPQIPARPLSLREHLNFQICLSQISEREKNIISLLIDSLNEDG
YLLQDLDELMEMLPAELGISLQDLQDALKYLQRLDPSGVGARNLQECLVLQLQALPDDAYRDQAIKVVQEYLEILASHDF
VQIKRLLACDDQSLRAIQKLIVNLNPKPGAQFNSQNERYIVPDITVTKKNGTWGANLNMAAIPRLNINHLYANILKQEHH
SSRGLVSQLNEAKWLIKNIQQRSSTILKVASAIVERQQQFFEHGEVAMRPLVLRDIAETLNLHESTVSRVTTQKFMYTPR
GIFELKYFFGSHVSTDSGGACSATAIRALIKQLIQEENPRKPLSDNRISTILEQQGIVVARRTIAKYRESIQIPAANLRK
SF

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=41644 NIT79A3_RS04465 WP_013965064.1 990038..991486(+) (rpoN/rpoN1) [Nitrosomonas sp. Is79A3]
ATGAAGCCGACACTCCAACTAAAGTTATCACAGCAACTTAGGCTTACCCCTCAGTTGCAGCAATCTATCCGGTTGCTGCA
ATTATCAACGCTTGAACTAAATCAGGAGATTGAGCGGATAGTGCAGGAAAATCCATTGCTGGAGTTGCGTGAGGACTGGA
ATGCTCCTGTGGCCGAGAATACATTCGAACATCCAGAATTTTCTGATGATGTGGCAGATTTTGATAGTGTGCCACCAGAA
GCGGCGGTTTCTGAAGTTCAAGCAGAGTCCGAGGAAAATTTTGAATCAGATTCTGAATGGCCGCAGGAAAACATTTTTTC
TCATGGCACTTATGAAGATGATGATCATGAAGCGCCGCAAATACCGGCGAGACCATTAAGCCTGCGTGAACATTTAAATT
TTCAGATTTGCCTGAGTCAAATTTCCGAGCGTGAGAAAAACATTATTAGCTTGTTGATTGATAGTCTGAATGAGGATGGC
TATCTGCTGCAAGATTTGGATGAACTGATGGAAATGCTGCCTGCAGAACTGGGCATCAGTCTGCAGGACTTGCAGGATGC
GCTCAAGTATTTGCAGCGCCTTGATCCATCTGGTGTGGGGGCACGTAATCTACAAGAATGTCTGGTACTGCAGTTGCAAG
CGTTGCCTGATGATGCTTATCGTGACCAGGCAATAAAAGTTGTGCAAGAATATTTGGAAATTTTGGCATCGCACGATTTT
GTCCAGATTAAGAGATTATTGGCATGTGATGATCAAAGTTTACGAGCTATTCAGAAGCTGATTGTTAATCTGAACCCTAA
ACCAGGCGCTCAGTTTAACTCTCAAAACGAACGCTATATTGTGCCAGATATTACGGTGACTAAAAAGAATGGAACTTGGG
GGGCAAACCTTAATATGGCCGCAATCCCACGCCTTAATATTAATCATCTCTATGCTAACATCCTGAAACAGGAACATCAT
TCTTCGCGTGGGCTAGTAAGCCAGTTGAATGAAGCGAAGTGGCTGATTAAGAATATTCAGCAGCGCTCAAGTACAATTCT
GAAAGTAGCCAGTGCGATTGTGGAACGTCAGCAGCAATTTTTTGAACATGGAGAAGTTGCTATGCGTCCGCTTGTATTAC
GAGATATTGCTGAAACCTTGAATCTACATGAATCAACAGTATCACGGGTAACTACACAAAAATTTATGTATACTCCGCGA
GGTATTTTTGAACTTAAATATTTTTTTGGCAGTCATGTCTCCACGGATTCAGGAGGAGCTTGTTCAGCAACGGCTATCCG
TGCATTGATCAAGCAACTGATACAGGAAGAGAACCCAAGGAAACCACTCAGCGATAACAGAATTTCAACTATTCTTGAAC
AACAAGGAATTGTTGTTGCTCGTCGAACCATTGCGAAGTACCGGGAATCAATACAAATTCCTGCAGCAAATCTTCGTAAA
TCATTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-480)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(121-311)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8GLM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.495

100

0.529

Multiple sequence alignment