Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NIT79A3_RS03360 Genome accession   NC_015731
Coordinates   740089..741438 (+) Length   449 a.a.
NCBI ID   WP_013964856.1    Uniprot ID   F8GK73
Organism   Nitrosomonas sp. Is79A3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 735089..746438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NIT79A3_RS03345 (Nit79A3_0676) - 736716..738368 (-) 1653 WP_348225705.1 ABC transporter transmembrane domain-containing protein -
  NIT79A3_RS03350 (Nit79A3_0677) rfbA 738584..739516 (+) 933 WP_013964854.1 glucose-1-phosphate thymidylyltransferase RfbA -
  NIT79A3_RS03355 (Nit79A3_0678) rfbC 739530..740078 (+) 549 WP_013964855.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  NIT79A3_RS03360 (Nit79A3_0679) radA 740089..741438 (+) 1350 WP_013964856.1 DNA repair protein RadA Machinery gene
  NIT79A3_RS03365 (Nit79A3_0680) - 741511..742296 (+) 786 WP_013964857.1 hypothetical protein -
  NIT79A3_RS03370 (Nit79A3_0681) - 742328..742870 (-) 543 WP_013964858.1 hypothetical protein -
  NIT79A3_RS03375 (Nit79A3_0682) - 742969..744351 (-) 1383 WP_013964859.1 DEAD/DEAH box helicase -
  NIT79A3_RS03380 (Nit79A3_0683) tyrS 744690..745892 (-) 1203 WP_013964860.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 48586.44 Da        Isoelectric Point: 8.0757

>NTDB_id=41643 NIT79A3_RS03360 WP_013964856.1 740089..741438(+) (radA) [Nitrosomonas sp. Is79A3]
MAKQKTIYSCTECGGQTLKWQGQCPHCQSWNTLIETIAEKSVSRFSPVSEVGQVQNLSTIEAREEPRYPTGVDELDRVLG
GGLVQGGVVLLGGDPGIGKSTLLLQALSHLSSHRSVLYVSGEESAHQVALRAKRLALNVETVNLFAEIQLEKIQSVLIER
KPAVAVIDSIQTIYSEVLQSAPGSVAQVRECAAQLTRLAKASGICIILVGHVTKEGALAGPRVLEHMVDTVLYFEGDMHS
SFRMIRAFKNRFGAVNELGVFSMSEKGLREVKNPSALFLSHHDTQVPGSCIMVTQEGTRPLLVEIQALVDEARSPNPRRL
CVGLEQNRLAMLLAVLHRHAGIPCFDQDVFVNAVGGVKITEPGADLAILLAVVSSLKNKPLAEKMIVIGEVGLAGEIRPV
QRGQERLKEAAKLGFKQAIIPLANKPKQAIPGMDIIAVARIRDAVAHMH

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=41643 NIT79A3_RS03360 WP_013964856.1 740089..741438(+) (radA) [Nitrosomonas sp. Is79A3]
ATGGCCAAGCAGAAAACGATCTACTCGTGTACTGAGTGTGGCGGGCAAACCTTAAAATGGCAAGGGCAGTGTCCGCATTG
CCAGTCATGGAATACCTTAATAGAAACCATTGCAGAGAAATCTGTATCGCGGTTCAGTCCAGTATCAGAAGTCGGTCAGG
TGCAAAATTTAAGTACTATCGAAGCGCGTGAAGAACCGCGCTATCCGACAGGTGTGGATGAGCTTGACCGGGTTCTTGGC
GGTGGTCTGGTGCAAGGCGGTGTGGTGCTATTAGGCGGGGATCCTGGTATTGGTAAGTCGACATTATTACTGCAAGCACT
CTCCCATTTGTCGAGTCATCGCTCTGTATTGTATGTAAGCGGTGAAGAATCCGCGCACCAAGTGGCATTACGCGCCAAGC
GTTTAGCGCTGAATGTGGAAACAGTTAATTTATTTGCGGAAATTCAACTGGAGAAAATCCAGTCTGTTCTGATTGAACGG
AAACCGGCTGTAGCCGTGATTGATTCTATTCAGACGATCTATTCGGAGGTATTACAGTCCGCGCCGGGATCTGTTGCGCA
AGTGCGCGAGTGTGCAGCACAATTAACACGCTTGGCGAAGGCAAGCGGAATTTGCATTATTCTGGTTGGTCATGTGACCA
AGGAAGGTGCGCTGGCTGGTCCGCGTGTGCTGGAACATATGGTCGATACGGTGCTGTATTTTGAAGGCGATATGCATTCC
AGTTTTCGCATGATCCGCGCTTTCAAGAATCGTTTTGGCGCGGTCAATGAATTGGGTGTGTTTTCTATGAGCGAAAAGGG
ATTGCGTGAGGTCAAGAATCCTTCGGCACTGTTTCTTTCGCATCATGACACGCAAGTGCCGGGATCGTGCATCATGGTCA
CACAAGAAGGCACGCGCCCGCTGTTGGTTGAAATACAGGCATTGGTCGATGAAGCGCGCTCGCCCAATCCGCGCCGGTTA
TGCGTCGGTTTGGAACAAAACCGCTTAGCCATGCTGCTCGCGGTATTGCACCGCCACGCGGGGATCCCCTGTTTCGATCA
AGACGTGTTCGTCAATGCCGTCGGCGGCGTGAAAATTACCGAACCGGGTGCTGATCTTGCGATTTTACTGGCGGTAGTCT
CGTCGCTGAAGAATAAACCGCTGGCGGAAAAAATGATCGTCATCGGTGAGGTGGGCCTGGCTGGCGAAATACGCCCGGTG
CAACGCGGACAGGAAAGACTGAAAGAAGCGGCGAAACTGGGTTTTAAACAAGCCATCATTCCGCTAGCCAATAAGCCCAA
ACAAGCCATTCCAGGCATGGACATCATTGCCGTTGCGCGGATCAGGGATGCAGTTGCACATATGCATTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(76-216)

Subunit ChlI of Mg-chelatase (ChlI)

(357-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F8GK73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.439

100

0.512
  radA Streptococcus mitis SK321

46.993

100

0.47
  radA Streptococcus pneumoniae Rx1

46.429

99.777

0.463
  radA Streptococcus pneumoniae D39

46.429

99.777

0.463
  radA Streptococcus pneumoniae R6

46.429

99.777

0.463
  radA Streptococcus pneumoniae TIGR4

46.429

99.777

0.463
  radA Streptococcus mitis NCTC 12261

48.227

94.209

0.454

Multiple sequence alignment