Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CNE_RS09915 Genome accession   NC_015726
Coordinates   2110883..2112244 (-) Length   453 a.a.
NCBI ID   WP_013957004.1    Uniprot ID   G0EYJ0
Organism   Cupriavidus necator N-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2105883..2117244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNE_RS09895 (CNE_1c20350) - 2106417..2107379 (-) 963 WP_013957001.1 MurR/RpiR family transcriptional regulator -
  CNE_RS09900 (CNE_1c20360) - 2107665..2109599 (+) 1935 WP_013957002.1 ATP-binding cassette domain-containing protein -
  CNE_RS09905 (CNE_1c20370) - 2109687..2110172 (-) 486 WP_010810635.1 disulfide bond formation protein B -
  CNE_RS09910 (CNE_1c20380) - 2110317..2110880 (-) 564 WP_013957003.1 GNAT family N-acetyltransferase -
  CNE_RS09915 (CNE_1c20390) radA 2110883..2112244 (-) 1362 WP_013957004.1 DNA repair protein RadA Machinery gene
  CNE_RS09920 (CNE_1c20400) alr 2112340..2113461 (-) 1122 WP_013957005.1 alanine racemase -
  CNE_RS09925 (CNE_1c20410) lplT 2114032..2115351 (+) 1320 WP_041227955.1 lysophospholipid transporter LplT -
  CNE_RS09930 (CNE_1c20420) - 2115394..2116533 (-) 1140 WP_013957007.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48386.69 Da        Isoelectric Point: 7.2389

>NTDB_id=41618 CNE_RS09915 WP_013957004.1 2110883..2112244(-) (radA) [Cupriavidus necator N-1]
MAKTKTVYTCTECGGTTPRWAGQCPHCQQWNTLVETVAESAANKRFQPLAASATVRKLSEIDAADVPRFSSGIDEFDRVL
GGGLVSGGVVLIGGDPGIGKSTLLLQALANLAGQRRVLYVSGEESGAQIALRAQRLGVESPSLGLLAEIQLEKIQATLEV
EKPEVAVIDSIQTLYSEALNSAPGSVAQVRECAAQLTRIAKSSGITIILVGHVTKEGSLAGPRVLEHIVDTVLYFEGDTH
SSHRLIRAFKNRFGAVNELGVFAMTERGLRGISNPSALFLSQHEETVPGSCVLVTQEGTRPLLVEIQALVDTAHVPNPRR
LAVGLEQNRLALLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLSIHSSMRNKPLPRGLVVFGEVGLAGEIRP
SPRGQERLKEAAKLGFTIAVIPKANAPKQKIDGLEVIAVERIEQAIDRVRHLD

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=41618 CNE_RS09915 WP_013957004.1 2110883..2112244(-) (radA) [Cupriavidus necator N-1]
TTGGCCAAGACCAAGACTGTCTATACGTGTACTGAATGCGGCGGCACCACGCCGCGCTGGGCCGGCCAGTGCCCGCATTG
CCAGCAGTGGAACACGCTGGTTGAGACGGTGGCCGAGTCCGCCGCGAACAAGCGCTTCCAGCCGCTGGCCGCGTCGGCCA
CGGTGCGCAAGCTGTCGGAGATCGATGCCGCTGACGTGCCGCGCTTCTCCAGCGGCATCGATGAATTCGACCGCGTGCTG
GGCGGCGGCCTGGTGTCGGGCGGGGTGGTGCTGATCGGCGGCGATCCCGGCATCGGCAAGTCCACGCTGCTGCTGCAGGC
CCTGGCCAACCTTGCGGGCCAGCGCCGCGTGCTCTATGTCAGCGGCGAAGAATCCGGTGCGCAGATCGCGCTGCGCGCGC
AGCGGCTGGGCGTGGAAAGCCCGTCGCTGGGGTTGCTTGCCGAAATCCAGCTGGAGAAGATCCAGGCCACGCTGGAAGTG
GAAAAGCCCGAAGTCGCGGTGATCGACTCGATCCAGACGCTGTACTCCGAGGCGCTGAACTCCGCACCGGGCTCGGTCGC
GCAGGTACGCGAATGCGCGGCGCAGCTGACGCGCATTGCCAAGAGCAGCGGCATCACCATCATCCTGGTCGGCCATGTGA
CCAAGGAAGGCAGCCTGGCGGGTCCGCGCGTGCTGGAGCATATCGTCGATACGGTGCTGTATTTCGAGGGCGATACCCAC
TCGTCGCACCGGCTGATCCGCGCCTTCAAGAACCGCTTCGGCGCGGTCAATGAGCTGGGCGTGTTTGCCATGACCGAGCG
CGGCCTGCGCGGCATCAGCAATCCGTCGGCGCTGTTCCTGTCGCAGCACGAAGAGACCGTGCCGGGCTCGTGCGTGCTGG
TGACGCAGGAGGGCACGCGTCCGCTGCTGGTTGAGATCCAGGCGCTGGTCGATACCGCCCATGTACCCAACCCGCGCCGG
CTGGCGGTGGGCCTTGAGCAGAACCGGCTGGCGCTGCTGCTGGCGGTGCTGCACCGGCACGCGGGCATTGCCTGCTTTGA
CCAGGACGTGTTCCTGAACGCGGTGGGCGGGGTCAAGATCACCGAGCCCGCCGCCGACCTCGCGGTGCTGCTGTCGATCC
ATTCATCGATGCGCAACAAGCCGCTGCCGCGCGGCCTGGTGGTGTTCGGCGAAGTCGGCCTGGCCGGCGAGATCCGCCCC
AGCCCGCGCGGGCAGGAGCGCCTGAAGGAAGCCGCCAAGCTGGGCTTTACGATCGCCGTGATTCCCAAGGCGAACGCGCC
CAAACAGAAGATCGACGGGCTCGAAGTGATCGCGGTGGAACGCATCGAGCAGGCCATCGACCGCGTGCGCCATCTGGACT
GA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(63-217)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0EYJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.106

99.779

0.51
  radA Streptococcus pneumoniae Rx1

46.813

100

0.47
  radA Streptococcus pneumoniae D39

46.813

100

0.47
  radA Streptococcus pneumoniae R6

46.813

100

0.47
  radA Streptococcus pneumoniae TIGR4

46.813

100

0.47
  radA Streptococcus mitis SK321

46.916

100

0.47
  radA Streptococcus mitis NCTC 12261

46.593

100

0.468

Multiple sequence alignment