Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CNE_RS01980 Genome accession   NC_015726
Coordinates   396729..398210 (-) Length   493 a.a.
NCBI ID   WP_013955472.1    Uniprot ID   G0EUQ7
Organism   Cupriavidus necator N-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 391729..403210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNE_RS01950 (CNE_1c03970) - 392059..392718 (-) 660 WP_013955470.1 LON peptidase substrate-binding domain-containing protein -
  CNE_RS01955 (CNE_1c03980) rapZ 392810..393694 (-) 885 WP_013955471.1 RNase adapter RapZ -
  CNE_RS01960 (CNE_1c03990) - 393812..394204 (-) 393 WP_010814383.1 PsiF family protein -
  CNE_RS01965 (CNE_1c04000) hprK 394367..395338 (-) 972 WP_010814384.1 HPr(Ser) kinase/phosphatase -
  CNE_RS01970 (CNE_1c04010) ptsN 395419..395874 (-) 456 WP_010814385.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CNE_RS01975 (CNE_1c04020) hpf 396330..396680 (-) 351 WP_010814386.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CNE_RS01980 (CNE_1c04030) rpoN/rpoN1 396729..398210 (-) 1482 WP_013955472.1 RNA polymerase factor sigma-54 Machinery gene
  CNE_RS01985 (CNE_1c04040) lptB 398346..399146 (-) 801 WP_013955473.1 LPS export ABC transporter ATP-binding protein -
  CNE_RS01990 (CNE_1c04050) lptA 399204..399854 (-) 651 WP_013955474.1 lipopolysaccharide transport periplasmic protein LptA -
  CNE_RS01995 (CNE_1c04060) lptC 399923..400552 (-) 630 WP_013955475.1 LPS export ABC transporter periplasmic protein LptC -
  CNE_RS02000 (CNE_1c04070) - 400596..401579 (-) 984 WP_013955476.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54698.94 Da        Isoelectric Point: 4.8616

>NTDB_id=41611 CNE_RS01980 WP_013955472.1 396729..398210(-) (rpoN/rpoN1) [Cupriavidus necator N-1]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEARADGAGDDDSYGDSGNGDDYGSGDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISARDKGLAI
FLIESLDDDGYLSASLEEICTELPEELEFEIDEVHAILTLLQSFDPPGVGARNAAECLALQLRRLTHPQRELALNIVSNH
LELLAVRDYTRLKKALQVDEVALKSAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKGGGGWIAQLNPDVMPRLRINDMY
AQILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=41611 CNE_RS01980 WP_013955472.1 396729..398210(-) (rpoN/rpoN1) [Cupriavidus necator N-1]
ATGAAACCGTCGCTACAGCTCCGTCTTTCCCAGCATCTGGCCCTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTGGAACTGCAGCAAGAGGTCGAACAGGCGCTGACGGAAAACCCGCTGCTCGAGCGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCGGCCGACGGCTCGGTCAACCTGCAGAGCGCACCGGCGCCCGCCCCGGCTGAGCCCCAG
GGCAATGGCGAGGCTCGCGCCGACGGCGCGGGTGACGACGACAGCTATGGCGACAGCGGCAACGGCGACGACTACGGCAG
CGGCGACTGGAGCCTGGACGACTTTGCCCGCCGCCCCCAGGGCGACGAGGATGAAAAAACGCCGATGCAGCTGCGCGAGG
CCGAGCCCACGCTGCGCGAGTACCTGATGGAGCAGCTCACGCCGCTGAAGATCTCGGCGCGCGACAAAGGCCTGGCCATC
TTCCTGATCGAATCGCTCGACGACGATGGCTACCTGAGCGCATCGCTTGAGGAGATCTGCACGGAGTTGCCGGAAGAACT
CGAGTTCGAGATCGACGAAGTCCACGCCATCCTCACGCTGCTGCAGAGTTTCGACCCGCCCGGCGTGGGTGCGCGCAACG
CCGCCGAGTGCCTGGCCCTGCAGTTGCGACGCCTGACGCACCCGCAACGCGAACTGGCGCTGAACATCGTTAGCAATCAC
CTGGAGTTGCTCGCAGTACGCGACTACACGCGGCTGAAGAAGGCGCTGCAGGTAGATGAAGTGGCACTGAAGTCCGCACA
CGAACTGATCCGCTCGCTGGCGCCCTACCCCGGCCATGCGTACAGCCGCCCGGAAGCGGATTTCGTGGTGCCGGACGTGT
TCGTGCGCAAGGGTGGCGGCGGCTGGATCGCGCAGCTCAATCCGGATGTGATGCCGAGGCTGCGCATCAACGATATGTAT
GCCCAAATCCTGCGTGGCGCGAAGGGTGAGTCCGGCACGGCCGGACTGCAGCAAAAGCTGCAAGAGGCGCGCTGGCTGAT
CAAGAACATCCAGCAAAGGTTCGACACAATCCTGCGTGTCTCGCAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCAGTC
ACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCAACCATCTCCCGG
GTGACGACCAATAAATACATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGCAGCCACGTGTCCACCGA
AACCGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCC
TTTCCGACAGTCGCATTGCCGAACTGCTGGGTGAGCAAGGCTTTGTTGTTGCGCGCCGCACTGTTGCCAAGTATCGCGAA
GCCCTGAAGATCCCCGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0EUQ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.441

100

0.807

Multiple sequence alignment