Detailed information NTG0041610

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: CNE_RS01865
• Genome accession: NC_015726
• Coordinates: 372967..374127 (-)
• Length: 386 a.a.
• NCBI ID: WP_013955457.1
• Uniprot ID: G0EUN5
• Organism: Cupriavidus necator N-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 367967..379127

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CNE_RS01840 (CNE_1c03750)	ntrB	368005..368910 (-)	906	WP_013955452.1	nitrate ABC transporter permease	-
CNE_RS01845 (CNE_1c03760)	-	368962..370272 (-)	1311	WP_013955453.1	CmpA/NrtA family ABC transporter substrate-binding protein	-
CNE_RS01850 (CNE_1c03770)	-	370638..371285 (-)	648	WP_013955454.1	ANTAR domain-containing response regulator	-
CNE_RS01855 (CNE_1c03780)	-	371541..372242 (+)	702	WP_013955455.1	fumarylacetoacetate hydrolase family protein	-
CNE_RS01860 (CNE_1c03790)	maiA	372247..372894 (+)	648	WP_013955456.1	maleylacetoacetate isomerase	-
CNE_RS01865 (CNE_1c03800)	pilA	372967..374127 (-)	1161	WP_013955457.1	signal recognition particle-docking protein FtsY	Machinery gene
CNE_RS01870 (CNE_1c03810)	-	374345..375892 (+)	1548	WP_013955458.1	pitrilysin family protein	-
CNE_RS01875 (CNE_1c03820)	-	375902..377284 (+)	1383	WP_013955459.1	pitrilysin family protein	-
CNE_RS01880 (CNE_1c03830)	rsmD	377367..378071 (+)	705	WP_013955460.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
CNE_RS01885 (CNE_1c03840)	coaD	378312..378797 (+)	486	WP_010814369.1	pantetheine-phosphate adenylyltransferase	-
CNE_RS01890 (CNE_1c03850)	-	378866..379126 (+)	261	WP_010814370.1	YfhL family 4Fe-4S dicluster ferredoxin	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 40872.64 Da
• Isoelectric Point: 7.3624

>NTDB_id=41610 CNE_RS01865 WP_013955457.1 372967..374127(-) (pilA) [Cupriavidus necator N-1]
MFSFWKKRKAEPAPVEAPAPAPAPVEVPAPVPAPAPTPAPVPAPAPAPVPAQVPPPAPAPVAAPVPVPAPAPAEALELVP
PPAQTAEAKLGWMHRLRTGLSKTSRNIGTLFVGVKVDEALFEELETALLMADAGVEATEYLLGELRKRVKAERIETAEGV
KAALRELLTQLLRPLEKTMALGREQPMVMMIAGVNGAGKTTSIGKLCKHFQRYDQKVLLAAGDTFRAAAREQLTIWGERN
NVTVVAQESGDPAAVIFDAVNAAKARGIDIVMADTAGRLPTQLHLMEELKKVKRVISKAMPSAPHEVLLVIDANTGQNAL
QQTRAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFKADEFADALLG

Nucleotide

• Length: 1161 bp

>NTDB_id=41610 CNE_RS01865 WP_013955457.1 372967..374127(-) (pilA) [Cupriavidus necator N-1]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCCGAGCCCGCGCCCGTCGAGGCGCCAGCCCCGGCCCCCGCTCCGGTCGAGGT
CCCCGCCCCTGTGCCGGCACCGGCCCCCACGCCTGCGCCAGTCCCTGCGCCCGCACCGGCACCGGTCCCAGCGCAAGTGC
CACCCCCTGCCCCAGCGCCCGTGGCAGCCCCGGTACCCGTGCCGGCGCCCGCACCGGCCGAAGCGCTCGAGCTGGTCCCG
CCGCCCGCCCAGACCGCTGAAGCGAAGCTGGGCTGGATGCATCGGCTGCGTACCGGCCTGTCCAAGACCAGCCGCAACAT
CGGCACGCTGTTCGTCGGCGTCAAGGTCGACGAGGCCCTGTTCGAAGAACTGGAAACCGCGCTGCTGATGGCCGATGCCG
GCGTCGAAGCCACCGAATACCTGCTGGGCGAGCTGCGCAAGCGCGTCAAGGCCGAGCGCATCGAGACCGCTGAAGGCGTC
AAGGCGGCACTGCGCGAGCTGCTGACCCAGCTGCTGCGCCCGCTGGAAAAGACCATGGCGCTGGGCCGCGAGCAGCCAAT
GGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGCGCTATG
ACCAGAAGGTGCTGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCGCGCGAGCAGCTGACGATCTGGGGCGAGCGCAAC
AACGTCACCGTGGTGGCGCAGGAAAGCGGCGACCCGGCGGCGGTGATCTTCGACGCCGTCAATGCGGCCAAGGCGCGCGG
CATCGACATCGTCATGGCCGACACCGCCGGCCGCCTGCCGACGCAGCTGCACCTGATGGAAGAGCTCAAGAAGGTCAAGC
GCGTGATCAGCAAGGCCATGCCGAGCGCGCCGCACGAGGTGCTGCTGGTGATCGACGCCAATACCGGCCAGAACGCCCTG
CAGCAAACCCGGGCCTTCGACGACGCGCTGGGCCTGACCGGGCTGATCGTGACCAAACTCGACGGCACCGCCAAGGGCGG
CATCCTGGCGGCGATCGCGCGCCAGCGCCCGGTGCCGGTCTACTTCATCGGCGTGGGCGAGAAAGTGGAAGACCTGCAAC
CGTTCAAGGCGGACGAGTTCGCCGACGCGCTGCTGGGATAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-386)

• SRP54-type protein, helical bundle domain (SRP54_N) (111-166)

3D structure

• AlphaFold DB: G0EUN5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.442	100	0.554