Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   THETH_RS01290 Genome accession   NC_015707
Coordinates   264485..265369 (+) Length   294 a.a.
NCBI ID   WP_013931580.1    Uniprot ID   F7YV58
Organism   Pseudothermotoga thermarum DSM 5069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259485..270369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THETH_RS01270 (Theth_0254) - 259676..260959 (+) 1284 WP_407635678.1 dicarboxylate/amino acid:cation symporter -
  THETH_RS01275 (Theth_0255) folP 260989..262164 (-) 1176 WP_013931577.1 dihydropteroate synthase -
  THETH_RS01280 (Theth_0256) - 262161..262667 (-) 507 WP_041446340.1 CoA pyrophosphatase -
  THETH_RS01285 (Theth_0257) - 262756..264480 (+) 1725 WP_013931579.1 proline--tRNA ligase -
  THETH_RS01290 (Theth_0258) pilA 264485..265369 (+) 885 WP_013931580.1 signal recognition particle-docking protein FtsY Machinery gene
  THETH_RS10430 (Theth_0259) - 265601..265789 (-) 189 WP_013931581.1 hypothetical protein -
  THETH_RS01295 (Theth_0260) - 265764..266051 (-) 288 WP_013931582.1 hypothetical protein -
  THETH_RS01300 (Theth_0262) - 266316..266978 (+) 663 WP_013931584.1 DUF2225 domain-containing protein -
  THETH_RS01305 (Theth_0263) - 266975..268687 (+) 1713 WP_013931585.1 3D domain-containing protein -
  THETH_RS01310 (Theth_0264) - 268709..269125 (+) 417 WP_013931586.1 Rrf2 family transcriptional regulator -
  THETH_RS01315 (Theth_0265) - 269130..270080 (+) 951 WP_013931587.1 GAF domain-containing protein -

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32023.42 Da        Isoelectric Point: 6.9884

>NTDB_id=41559 THETH_RS01290 WP_013931580.1 264485..265369(+) (pilA) [Pseudothermotoga thermarum DSM 5069]
MGFFEKLKASLEKTRKSIFEGLGKLLKGKKIDQELLEQLEEKLIMADVGYETTQEILQKLEKENTEDALETLKKVLVEIL
SNGNQLVVPNAKPFVIMVVGVNGTGKTTSVAKLGAYFQNLGKTVVLAAADTFRAAAIEQLQEWGKRINCTVIAHSEGADP
AAVAFDAVNHAKAKEKDVVIIDTAGRLHTKKNLMEELRKIHRVVGKVVENAPHEVLLVIDATTGQNGLVQAKVFKEMVNI
TGIILTKLDGTAKGGIALAIKKELGVPIKFIGVGESLEDFKPFDPTLFVEALLE

Nucleotide


Download         Length: 885 bp        

>NTDB_id=41559 THETH_RS01290 WP_013931580.1 264485..265369(+) (pilA) [Pseudothermotoga thermarum DSM 5069]
ATGGGATTTTTCGAAAAATTGAAAGCAAGCCTTGAAAAAACTCGAAAAAGTATTTTTGAGGGACTTGGGAAACTTTTAAA
AGGCAAAAAAATAGATCAAGAATTGCTCGAGCAACTTGAAGAGAAACTGATAATGGCAGACGTGGGCTATGAAACAACCC
AAGAGATTCTTCAAAAGCTTGAAAAAGAAAACACAGAAGATGCGCTTGAAACGTTGAAAAAAGTGCTTGTGGAAATTCTT
TCAAATGGAAATCAACTTGTTGTTCCGAATGCAAAACCTTTTGTGATAATGGTTGTGGGTGTCAACGGGACAGGAAAAAC
AACCAGCGTGGCAAAACTTGGCGCCTATTTTCAAAATCTTGGCAAAACAGTGGTTCTTGCCGCCGCGGACACCTTCAGAG
CAGCTGCCATCGAACAACTTCAAGAATGGGGTAAAAGAATAAATTGTACAGTAATAGCACACAGTGAAGGCGCAGACCCG
GCTGCCGTAGCCTTCGATGCGGTAAATCACGCAAAGGCTAAAGAAAAGGATGTGGTGATAATAGATACAGCCGGAAGACT
TCATACAAAAAAGAACCTTATGGAAGAACTTCGAAAAATACACAGAGTTGTTGGAAAGGTTGTTGAAAATGCCCCCCACG
AGGTATTGCTTGTGATAGACGCAACGACAGGGCAAAACGGTCTTGTTCAAGCAAAAGTTTTCAAGGAAATGGTGAACATA
ACCGGTATAATTCTGACAAAACTCGATGGTACGGCAAAAGGAGGAATAGCCCTTGCGATAAAGAAAGAGCTCGGCGTTCC
AATAAAGTTCATAGGTGTTGGAGAAAGTTTGGAGGATTTCAAACCTTTCGATCCAACACTGTTTGTGGAAGCTTTACTTG
AATAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(93-293)

SRP54-type protein, helical bundle domain (SRP54_N)

(20-76)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F7YV58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

46.865

100

0.483

Multiple sequence alignment